Synthesis method and application of a patterned silicon dioxide nanostructure
A silicon dioxide and nanostructure technology, applied in the direction of silicon dioxide, silicon oxide, nanotechnology, etc., can solve the problems of limiting the accuracy of silicon dioxide nanostructures, limiting the complexity of silicon dioxide nanostructures, etc., and achieve wide application foreground effect
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Embodiment 1
[0067] (1) Assembly of triangular DNA origami structures with extended strands
[0068] Mix DNA template strand, folding helper strand, and folding helper strand with extension strand at a molar ratio of 1:10:10 in 1×TAE / Mg 2+ Anneal in buffer (pH=8.0), the annealing condition is from 95°C to 25°C, every 5°C is a gradient, and each gradient stays for 5min;
[0069] After the annealing is completed, add the DNA origami structure to a 100kDa spin column and add 1×TAE / Mg 2+ Buffer (pH=8.0), centrifuged to remove excess short-chain DNA;
[0070] (2) Hybridization of the complementary strand with the extended strand of the triangular DNA origami structure
[0071] The purified DNA origami structure was mixed with the complementary strand at a molar ratio of 1:360, and mixed in 1×TAE / Mg 2+ Annealing was carried out under the condition of buffer solution (pH=8.0), the annealing condition was from 45°C to 25°C, every 5°C was a gradient, each gradient was kept for 5min, and 6 cycles...
Embodiment 2
[0077] (1) Assembly of triangular DNA origami structures with extended strands
[0078]Mix DNA template strand, folding helper strand, and folding helper strand with extension strand at a molar ratio of 1:10:10 in 1×TAE / Mg 2+ Anneal in buffer (pH=8.0), the annealing condition is from 95°C to 25°C, every 5°C is a gradient, and each gradient stays for 5min;
[0079] After annealing is complete, add the DNA origami structure to a 100kDa spin column and add 1×TAE / Mg 2+ Buffer (pH=8.0), centrifuged to remove excess short-chain DNA;
[0080] (2) Hybridization of the complementary strand with the extended strand of the triangular DNA origami structure
[0081] The purified DNA origami structure was mixed with the complementary strand at a molar ratio of 1:540, and mixed in 1×TAE / Mg 2+ Annealing was carried out under the condition of buffer solution (pH=8.0), the annealing condition was from 45°C to 25°C, every 5°C was a gradient, each gradient was kept for 5min, and 6 cycles were ...
Embodiment 3
[0087] (1) Assembly of triangular DNA origami structures with extended strands
[0088] Mix DNA template strand, folding helper strand, and folding helper strand with extension strand at a molar ratio of 1:10:10 in 1×TAE / Mg 2+ Anneal in buffer (pH=8.0), the annealing condition is from 95°C to 25°C, every 5°C is a gradient, and each gradient stays for 5min;
[0089] After annealing is complete, add the DNA origami structure to a 100kDa spin column and add 1×TAE / Mg 2+ Buffer (pH=8.0), centrifuged to remove excess short-chain DNA;
[0090] (2) Hybridization of the complementary strand with the extended strand of the triangular DNA origami structure
[0091] The purified DNA origami structure was mixed with the complementary strand at a molar ratio of 1:720, and mixed in 1×TAE / Mg 2+ Annealing was carried out under the condition of buffer solution (pH=8.0), the annealing condition was from 45°C to 25°C, every 5°C was a gradient, each gradient was kept for 5min, and 6 cycles were...
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