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Method for preparing graphene biosensor

A biosensor and graphene technology, applied in the field of electrochemistry, can solve the problems of graphene and enzymes falling off easily, low sensitivity, poor sensor reproducibility, etc., achieve precise control of size and distribution density, good repeatability, and overcome enzymes Or the effect that the antibody is easy to fall off

Active Publication Date: 2012-06-27
盐城福万家保温板有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide a new method for preparing graphene biosensors for the existing graphene biosensors, which have the shortcomings of graphene and enzymes that are easy to fall off, low sensitivity and poor reproducibility of sensor production.

Method used

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  • Method for preparing graphene biosensor
  • Method for preparing graphene biosensor

Examples

Experimental program
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Effect test

Embodiment 1

[0025] Immerse the treated gold electrode in 0.05mg / mL graphene oxide dispersion, control the potential at -1.2V for 50s at 20°C, take out the electrode, wash it with water, dry it at room temperature, and place it in 0.05mmol / L chlorine Electrodeposition was carried out at -0.25V for 50s at 20°C in gold acid solution, and the electrode was taken out, washed with water, and dried at room temperature. After the above electrodeposition operation was cycled 20 times, the modified electrode was placed into 3 mL containing 2,5-bis(2-thiophene)-1-pyrrole p-benzoate (1 mmol / L) and tetrabutylammonium perchlorate (0.1 mol / L). L) in dichloromethane solution, control the potential between 0 and 1V to carry out polymerization by cyclic voltammetry, scan six times at a rate of 100mV / s, take out the electrode, wash with water, and dry at room temperature. The modified electrode was activated in 20mmol / L EDC / NHS solution for 4h, and then soaked in 10mg / mL catalase for 24h, the detection limi...

Embodiment 2

[0027] Immerse the treated gold electrode in 0.02mg / mL graphene oxide dispersion, control the potential at -1.0V for 20s at 0°C, take out the electrode, wash it with water, dry it at room temperature, and place it in 0.01mmol / L chlorine Electrodeposition was carried out at -0.3V at 25°C for 40s in gold acid solution, and the electrode was taken out, washed with water, and dried at room temperature. After 30 cycles of the above electrodeposition operation, the modified electrode was placed in 3 mL containing 2,5-bis(2-thiophene)-1-pyrrole p-benzoate (1 mmol / L) and tetrabutylammonium chloride (0.1 mol / L ) in a dichloromethane solution, control the potential between 0 and 1V and carry out polymerization by cyclic voltammetry, scan six times at a rate of 200mV / s, take out the electrode, wash with water, and dry at room temperature. The modified electrode was activated in 20mmol / L EDC / NHS solution for 4h, and then soaked in 10mg / mL glucose oxidase for 24h. The detection limit of th...

Embodiment 3

[0029] Immerse the treated gold electrode in a solution containing 0.02mg / mL graphene oxide and 0.1mol / L potassium chloride, control the potential at -1.0V for 20s at 40°C, take out the electrode, wash it with water, dry it at room temperature, and put it away. In 0.01mmol / L chloroauric acid solution, electrodeposit at -0.4V at 0°C for 20s at a controlled potential, take out the electrode, wash with water, and dry at room temperature. After 50 cycles of the above electrodeposition operation, the modified electrode was placed in 3 mL of 2,5-di(2-thiophene)-1-pyrrole p-benzoate (1.0 mmol / L) and tetraoctylamine tetrafluoroborate. In dichloromethane solution (0.1mol / L), the polymerization was carried out by controlling the potential between 0 and 1V by cyclic voltammetry, scanning six times at a rate of 300mV / s, taking out the electrode, washing with water, and drying at room temperature. The modified electrode was activated in 20mmol / L EDC / NHS solution for 4h, and then in 20mg / mL...

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Abstract

The invention relates to a method for preparing a graphene biosensor, belonging to the technical field of electrochemistry. The method comprises the following steps of: immersing a processed gold electrode into graphene oxide and a sodium sulphate solution, electrically depositing the electrode through a control electric potential, taking out the electrode, washing the electrode by using water, after drying the electrode at room temperature, putting the electrode in a chloroauric acid solution, electrically depositing the electrode through the control electric potential, taking out the electrode, washing the electrode by using water, and drying the electrode at room temperature; putting a modified electrode in a conductive polymer monomer and a supporting electrolyte solution, polymerizing the electrode through the control electric potential by adopting a cyclic voltammetry, taking out the electrode, washing the electrode by using water, and drying the electrode at room temperature; and activating the modified electrode in an EDC / NHS (Dichloroethane / N-Hydroxysuccinimide) solution, and immersing the modified electrode in a vomiting toxin antibody. The method disclosed by the invention is used for fixing graphene, gold nanoparticles and conducting polymers through electro-deposition; therefore, the method is very green and environment-friendly; furthermore, the thickness of a coating and sizes and distribution densities of the gold nanoparticles can be precisely controlled, thus, the batch production repeatability of the modified electrode is good.

Description

technical field [0001] The invention relates to a preparation method of a graphene biosensor, which belongs to the technical field of electrochemistry. Background technique [0002] Graphene is a crystal with a hexagonal honeycomb lattice structure formed by the close packing of single-layer carbon atoms. Its unique two-dimensional structure makes it have excellent electrical, thermal, mechanical and chemical properties, but there are large van der Waals gaps between graphene sheets. It is prone to accumulation and aggregation, which limits its application in many aspects. In order to solve the above problems, metal nanoparticles such as gold are doped between graphene sheets, which not only effectively prevents graphene sheets from returning to graphite crystals due to aggregation, but also greatly improves the electronic conductivity of graphene materials ( Zhong, Z.Y., Wu, W., Wang, D., Wang, D., Shan, J.L., Qing, Y., Zhang, Z.M., Biosensors and Bioelectronics, 2010, 25:...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/327
Inventor 李在均夏前芳赵静杨雪李洋
Owner 盐城福万家保温板有限公司
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