Biological enzyme preparation method

Abstract

The invention discloses a biological enzyme preparation method. The method specifically comprises the following steps of S1, raw material culture, wherein 8.5 g of fresh bran, 3.5 g of rice bran, 2.0g of bean cake powder, 0.5 g of (NH4)2SO4, 0.08 g of K2HPO4, 0.05 g of CaCl2 and 9.5 mL of water are mixed in a 500 mL beaker. The invention relates to the technical field of biological enzymes. The biological enzyme preparation method comprises the following steps of mixing 8.5 g of the fresh bran, 3.5 g of the rice bran, 2.0 g of the bean cake powder, 0.5 g of the (NH4)2SO4, 0.08 g of the K2HPO4, 0.05 g of the CaCl2 and 9.5 mL of the water in the 500 mL beaker, and mixing and stirring a mixed solvent in the beaker by using a magnetic stirrer. A biological enzyme is prepared with the method,so that the acquisition rate of the biological enzyme can be effectively improved; in addition, drying box treatment is performed firstly before airflow drying, so that the activity of the biologicalenzyme can be improved to the relatively great extent and the waste of the biological enzyme preparation raw materials is reduced; the appearance quality of a biological enzyme product is effectivelyimproved by adopting a liquid fermentation method; and the impurity content of the prepared biological enzyme is greatly reduced by adopting ion exchange chromatography and gel filtration chromatography.

Description

technical field [0001] The invention relates to the technical field of biological enzymes, in particular to a production method of biological enzymes. Background technique [0002] Biological enzyme is a catalytic organic substance produced by living cells. Most of it is protein, and a small part is RNA. Biological enzyme is a non-toxic and environmentally friendly biocatalyst. Its chemical essence is protein. The production of enzyme It has a history of more than 80 years at home and abroad. In the 1980s, bioengineering, as a new high-tech technology, has developed rapidly in our country. The manufacturing and application fields of enzymes have gradually expanded, and the application of enzymes in the textile industry has also increased. It is becoming more and more mature. It has been mainly used in the desizing of cotton fabrics and degumming of silk in the past, and now it is widely used in various fields of textile dyeing and finishing, which reflects the superiority of...

AI Technical Summary

Problems solved by technology

[0005] Aiming at the deficiencies of the prior art, the present invention provides a method for producing biological enzymes, which solves the problem that the acquisition rate of the biological enzymes produced by the existing methods is low, and the activity of the biological enzymes is relatively poor, resulting in a large amount of waste of raw materials. In addition, the current Manufacturers can only obtain solid enzyme preparations by direct drying and crushing or liquid enzyme preparations after soaking in water and pressure filtration. The appearance of the product is rough, the quality of the finished product is unstable, and the content of impurities is high.

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