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African swine fever virus MGF-505R gene fluorescent PCR detection reagent and reagent kit, and application of reagent kit

A technology of MGF-505R and African swine fever virus, which is applied in the direction of resistance to vector-borne diseases, measurement/testing of microorganisms, DNA/RNA fragments, etc., can solve the problem that the differential diagnosis of African swine fever immunized pigs is still blank

Active Publication Date: 2020-06-23
CHINESE ACAD OF INSPECTION & QUARANTINE
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, there is currently no rapid and effective detection kit for the African swine fever virus MGF-505R gene
In addition, the differential diagnosis for African swine fever vaccinated pigs with MGF-505R gene deletion is still blank

Method used

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  • African swine fever virus MGF-505R gene fluorescent PCR detection reagent and reagent kit, and application of reagent kit
  • African swine fever virus MGF-505R gene fluorescent PCR detection reagent and reagent kit, and application of reagent kit
  • African swine fever virus MGF-505R gene fluorescent PCR detection reagent and reagent kit, and application of reagent kit

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Embodiment

[0040] The following examples are used herein to demonstrate preferred embodiments of the invention. It should be appreciated by those of skill in the art that the techniques disclosed in the examples which follow represent techniques discovered by the inventors to be employed in the practice of the invention, and thus can be considered preferred modes for its practice. However, those skilled in the art should understand from this specification that many modifications can be made to the specific embodiments disclosed herein, and the same or similar results can still be obtained without departing from the spirit or scope of the present invention.

[0041] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by those skilled in the art to which this invention belongs, and the disclosures cited herein and their cited materials are all incorporated by reference .

[0042] Those skilled in the art will recognize, or ...

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Abstract

The invention discloses an African swine fever virus (ASFV) MGF-505R gene fluorescent PCR detection reagent and reagent kit, and an application of the reagent kit, and belongs to the field of virus detection. An MGF-505R gene is used as a reference, a pair of special PCR primers and a TaqMan fluorescent probe are designed and optimized, plasmids containing an MGF-505R gene sequence are used as positive control, and a standard curve is made. The analysis sensibility of the method can achieve 1.36 copies. When being used for detecting African swine fever viruses and identifying MGF-505R gene deletion vaccine strains and natural infection strains, the African swine fever virus MGF-505R gene fluorescent PCR detection reagent and reagent kit are high in susceptibility and high in specificity and suitable for fast detection.

Description

technical field [0001] The invention relates to the field of virus detection, in particular to an African swine fever virus MGF-505R gene fluorescent PCR detection reagent, kit and application thereof. Background technique [0002] African swine fever (ASF) is a severe, hemorrhagic infectious disease of pigs caused by African swine fever virus (ASFV). Montgomery was first discovered in Kenya, Africa in 1921. ASFV virus has an envelope with a diameter of 175-215nm, and the nucleocapsid is icosahedral. The genome is composed of single-molecule linear double-stranded DNA, with a size of 170-190Kb, containing 151-167 open reading frames (ORFs), of which about 30% of ORFs have multiple copies, that is, multi-gene families, including MGF100, MGF110, For MGF300, MGF360, MGF505 / 530, and p22, the copy numbers of ORFs in different isolated strains are also different, and multiple copies of MGFs family gene coding may provide the virus with an advantage in selective evolution. As im...

Claims

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Application Information

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IPC IPC(8): C12Q1/70C12Q1/686C12N15/11
CPCC12Q1/701C12Q1/686C12Q2600/166C12Q2563/107C12Q2545/113Y02A50/30
Inventor 邓俊花吴绍强王彩霞冯春燕林祥梅
Owner CHINESE ACAD OF INSPECTION & QUARANTINE
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