Novel coronavirus nucleic acid testing primer pair with anti-mutation performance, kit and application of primer pair

Abstract

The invention provides a novel coronavirus nucleic acid testing primer pair with anti-mutation performance, a kit and application of the primer pair, and belongs to the technical field of bioengineering and molecular testing. Through preferred design, the primer pair provided by the invention has the excellent anti-mutation performance, and thereby, the situations that testing sensitivity is reduced and even the false negative phenomenon occurs due to gene mutation of a novel coronavirus can be effectively avoided, and rapid high-sensitivity and high-accuracy real-time fluorescence quantification polymerase chain reaction (PCR) testing of the novel coronavirus SARS-CoV-2 is realized; and the primer pair is convenient for a primary level to operate and apply, and accordingly, the primer pair has significant application value in novel coronavirus SARS-CoV-2 epidemic diagnosis and epidemiological investigation.

Description

technical field [0001] The invention belongs to the technical field of bioengineering and molecular detection, and in particular relates to a novel coronavirus nucleic acid detection primer pair with anti-mutation performance, a kit and an application thereof. Background technique [0002] The information disclosed in this background section is only intended to increase the understanding of the general background of the present invention, and is not necessarily taken as an acknowledgment or any form of suggestion that the information constitutes the prior art already known to those skilled in the art. [0003] The new respiratory disease that broke out since December 2019 has more than 2 million confirmed cases worldwide as of mid-April 2020. The main clinical symptoms are fever, fatigue, and dry cough. After isolation and identification, the pathogen was confirmed to be a new type of coronavirus, which was named "SARS-CoV-2" by the International Committee on Taxonomy of Vir...

AI Technical Summary

Problems solved by technology

The second is that it is not easy to be detected by existing detection technologies. Both of these results will cause virus carriers to spread around, resulting in widespread infection

In clinical use, it was found that the specificity of the N gene is relatively poor, and many samples are only positive for the N gene, while the other gene is negative

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