Novel coronavirus nucleic acid testing primer pair with anti-mutation performance, kit and application of primer pair

A coronavirus and primer pair technology, applied in the detection/testing of microorganisms, microorganisms, and methods based on microorganisms, etc., can solve the problems of virus carriers spreading around, large-scale infection, poor specificity of N genes, etc., and achieve simple detection methods Fast, avoid detection sensitivity reduction or even false negative phenomenon, strong specificity effect

Active Publication Date: 2020-06-23
山东仕达思生物产业有限公司 +2
View PDF2 Cites 7 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The second is that it is not easy to be detected by existing detection technologies. Both of these results will cause virus carriers to spread around, resulting in widespread infection
In clinical use, it was found that the specificity of the N gene is relatively poor, and many samples are only positive for the N gene, while the other gene is negative

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Novel coronavirus nucleic acid testing primer pair with anti-mutation performance, kit and application of primer pair
  • Novel coronavirus nucleic acid testing primer pair with anti-mutation performance, kit and application of primer pair
  • Novel coronavirus nucleic acid testing primer pair with anti-mutation performance, kit and application of primer pair

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0155] The preparation method of the above kit also belongs to the protection scope of the present invention.

[0156] Therefore, in another specific embodiment of the present invention, the preparation method of the above kit is as follows (I) or (II):

[0157] (1) Each primer and probe of each of the above-mentioned primer pairs are individually packaged;

[0158] (II) The primers and probes of each of the above primer pairs are mixed together in proportion.

[0159] In yet another specific embodiment of the present invention, a method for identifying or assisting in identifying the new coronavirus SARS-CoV-2 is provided, comprising the following steps: using the cDNA of the virus to be tested as a template, using the above primer pair and / or the above reagent box for fluorescent quantitative PCR;

[0160] If a positive amplification curve is shown, the virus to be tested is a candidate new coronavirus SARS-CoV-2; if the above conditions are not met, the virus to be tested...

Embodiment 1

[0169] 1. Experimental materials

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention provides a novel coronavirus nucleic acid testing primer pair with anti-mutation performance, a kit and application of the primer pair, and belongs to the technical field of bioengineering and molecular testing. Through preferred design, the primer pair provided by the invention has the excellent anti-mutation performance, and thereby, the situations that testing sensitivity is reduced and even the false negative phenomenon occurs due to gene mutation of a novel coronavirus can be effectively avoided, and rapid high-sensitivity and high-accuracy real-time fluorescence quantification polymerase chain reaction (PCR) testing of the novel coronavirus SARS-CoV-2 is realized; and the primer pair is convenient for a primary level to operate and apply, and accordingly, the primer pair has significant application value in novel coronavirus SARS-CoV-2 epidemic diagnosis and epidemiological investigation.

Description

technical field [0001] The invention belongs to the technical field of bioengineering and molecular detection, and in particular relates to a novel coronavirus nucleic acid detection primer pair with anti-mutation performance, a kit and an application thereof. Background technique [0002] The information disclosed in this background section is only intended to increase the understanding of the general background of the present invention, and is not necessarily taken as an acknowledgment or any form of suggestion that the information constitutes the prior art already known to those skilled in the art. [0003] The new respiratory disease that broke out since December 2019 has more than 2 million confirmed cases worldwide as of mid-April 2020. The main clinical symptoms are fever, fatigue, and dry cough. After isolation and identification, the pathogen was confirmed to be a new type of coronavirus, which was named "SARS-CoV-2" by the International Committee on Taxonomy of Vir...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/70C12Q1/6851C12N15/11C12R1/93
CPCC12Q1/6851C12Q1/701C12Q2531/113C12Q2563/107
Inventor 谷立川胡玮谢晓鸿谢时灵董宏杰张俊梅王宏伟张坤迪张冯瑜何青王峰
Owner 山东仕达思生物产业有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap