Preparation method of fermented type Evernia extract for cigarettes

An extract and fermented technology, which is applied in the field of preparation of fermented tree flower extracts for tobacco, to achieve the effects of good coordination of tobacco aroma, outstanding aroma, and reduction of woody odor

Pending Publication Date: 2020-06-26
YUNNAN REASCEND TOBACCO TECH GRP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

But up to now, do not see the report of preparing tobacco spices with tree flower all the time

Method used

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  • Preparation method of fermented type Evernia extract for cigarettes

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] A preparation method of fermented tree flower extract for tobacco, the method is as follows:

[0026] (1) Inoculate the Aspergillus niger strain RSZZ-6-1 taken from tobacco leaves used in the laboratory in PDA solid medium, and cultivate it with shaking at 35°C and 160r / min for 24 hours to obtain the activated Aspergillus niger strain;

[0027] (2) Grinding dried tree flowers and tobacco stems respectively, passing through a 20-mesh sieve, mixing, weighing 50 g, adding 150 ml of distilled water, and autoclaving to obtain a mixture for subsequent use;

[0028] (3) Inoculate the Aspergillus niger activated bacterial classification prepared by step (1) in the mixture prepared by step (2), the inoculum amount is 0.5% of the mixture quality; fermentation and cultivation after inoculation, the culture condition is 35 ℃, 160 / min shaking culture for 72 hours;

[0029] (4) Extract the fermented product obtained by the fermentation culture with ethanol with a mass concentration...

Embodiment 2

[0033] A preparation method of fermented tree flower extract for tobacco, the method is as follows:

[0034] (1) The Aspergillus niger strain RSZZ-6-1 used in the experiment was inoculated in PDA solid medium, and cultured with shaking at 36°C and 170r / min for 48 hours to obtain the activated Aspergillus niger strain;

[0035] (2) After the tree flower and the tobacco stem are fully dried, they are respectively pulverized, passed through a 20-mesh sieve, mixed, weighed 50 g, added 150 ml of distilled water, and sterilized by high pressure to obtain a mixture, which is set aside;

[0036] (3) Inoculate the prepared Aspergillus niger activated strain into the prepared mixture, the inoculum amount is 1.0% of the mixture quality, ferment and cultivate after inoculation, and the culture conditions are 35° C., 160 / min shaking culture for 24 hours;

[0037] (4) Extract the fermented product obtained by the fermentation culture with ethanol with a mass concentration of 40%, the extrac...

Embodiment 3

[0040] (1) The Aspergillus niger strain RSZZ-6-1 used in the experiment was inoculated in PDA solid medium, and cultured with shaking at 37°C and 160r / min for 24 hours to obtain the activated Aspergillus niger strain;

[0041] (2) After the tree flower and the tobacco stem are fully dried, they are respectively pulverized, passed through a 20-mesh sieve, mixed, weighed 50g, added 200ml of distilled water, and sterilized by autoclaving to obtain the mixture, which is set aside;

[0042] (3) Inoculate the prepared Aspergillus niger activated bacterial classification in the prepared mixture, the inoculum amount is 1.5% of the mixture quality, ferment and cultivate after inoculation, and the culture condition is 35 ℃, 160 / min shaking culture for 48 hours;

[0043] (4) extracting the fermented product obtained by the fermentation culture with ethanol with a mass concentration of 50%, the extraction temperature is 95° C., and the extraction time is 4 hours;

[0044] (5) Centrifuge t...

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Abstract

The invention discloses a preparation method of a fermented type Evernia extract for cigarettes. The preparation method comprises the following steps of: firstly, carrying out activated culture on anAspergillus niger strain RSZZ-6-1 to obtain an Aspergillus niger activated strain; then, respectively crushing dried Evernia and tobacco stems, then adding water with the volume being 3-5 times that of the crushed Evernia and tobacco stems, and carrying out sterilizing to obtain a mixture; inoculating the Aspergillus niger activated strain into the mixture for fermentation culture; performing extracting treatment on an obtained fermented product by using ethanol; carrying out centrifugal treatment on an extracting solution, and concentrating a supernatant into an extractum; and adding propylene glycol with the mass ratio of 2-20% of the concentrated extractum into the concentrated extractum to obtain the required Evernia extract. The Evernia extract prepared by the preparation method disclosed by the invention can retain the unique fragrance of the Evernia, has good coordination with the fragrance of the cigarettes, and can improve the taste of the cigarettes when being added into thecigarettes.

Description

technical field [0001] The invention belongs to the technical field of preparation of flavorings for tobacco, and in particular relates to a method for preparing a fermented tree flower extract for tobacco prepared by using aroma-producing bacteria on the surface of tobacco leaves. Background technique [0002] Tobacco flavoring determines the unique style of cigarettes, and the addition of tobacco flavors and fragrances plays an important role in the taste of cigarettes. There are many types of flavors and fragrances for tobacco, each with its own characteristics, and most of them are spices extracted from natural plants, which can give cigarettes the original aroma of natural plants. However, these tobacco flavors and fragrances extracted from natural plants are basically directly extracted from a single raw material. Not only the fragrance is single, but the coordination between some flavors and fragrances and shredded tobacco needs to be improved. [0003] With the cont...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12P1/02A24B15/26A24B3/12C12R1/685
CPCC12N1/14C12P1/02A24B15/26A24B3/12C12R2001/685C12N1/145
Inventor 曹环崔洪亮张世东吴平艳刘维涓卫青
Owner YUNNAN REASCEND TOBACCO TECH GRP
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