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A kind of enzyme-linked immunoassay detection kit for novel coronavirus IgG antibody

A technology of enzyme-linked immunoassay and detection kit, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., and can solve the problems of long detection time, difficulty in large-scale development, and high requirements for sample collection

Active Publication Date: 2020-09-08
BIOSCIENCE (TIANJIN) DIAGNOSTIC TECH CO LTD +1
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] At present, the detection methods of clinical laboratories mainly rely on nucleic acid detection, but nucleic acid detection should be carried out in qualified and qualified laboratories. There are shortcomings such as long detection time, high requirements for sample collection, many steps, high requirements for venues and equipment, and it is difficult to carry out large-scale

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  • A kind of enzyme-linked immunoassay detection kit for novel coronavirus IgG antibody
  • A kind of enzyme-linked immunoassay detection kit for novel coronavirus IgG antibody
  • A kind of enzyme-linked immunoassay detection kit for novel coronavirus IgG antibody

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Embodiment Construction

[0052] Unless otherwise defined, the technical terms used in the following embodiments have the same meaning as commonly understood by those skilled in the art to which the present invention belongs. The test reagents used in the following examples, unless otherwise specified, are conventional biochemical reagents; the experimental methods, unless otherwise specified, are conventional methods.

[0053] The present invention will be described in detail below in conjunction with examples.

[0054] An ELISA detection kit for novel coronavirus IgG antibody, including anti-FITC antibody ELISA plate, FITC-labeled novel coronavirus antigen, horseradish peroxidase-labeled mouse anti-human IgG monoclonal antibody, reference material, negative Control, Positive Control, Substrate Solution, Concentrated Wash Solution, Normal Saline, Reaction Tube.

[0055] The preparation method of the anti-FITC antibody microtiter plate is as follows: dilute the anti-FITC antibody to 1-5 μg / mL with 0.0...

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Abstract

The invention provides an ELISA detection kit for IgG antibodies against novel coronavirus, comprising an anti-FITC antibody microtiter plate, FITC-labeled novel coronavirus antigen, and horseradish peroxidase-labeled mouse anti-human IgG monoclonal antibody. Compared with the existing nucleic acid detection kits, the kit of the present invention has the advantages of simple operation, high throughput, high sensitivity, high speed, low cost, and low requirements for laboratories.

Description

technical field [0001] The invention belongs to the technical field of immunoassay and detection, and in particular relates to an enzyme-linked immunoassay detection kit for novel coronavirus IgG antibody. Background technique [0002] Coronaviruses belong to single-stranded positive-strand RNA viruses. There are six kinds of coronaviruses known to infect humans in the past, namely HCoV-229E, HCoV-OC43, SARSr-CoV, HCoV-NL63, HCoV-HKU1 and MERSr-CoV. The new coronavirus (2019-nCoV) belongs to the seventh category. [0003] Novel coronavirus pneumonia is an acute infectious pneumonia whose pathogen is a new type of coronavirus not previously found in humans, namely 2019-nCoV. Transmission through respiratory droplets and close contact is the main route of transmission, and there is a possibility of aerosol transmission in relatively closed environments exposed to high concentrations of aerosols for a long time. The initial symptoms of patients are mostly fever, fatigue and d...

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/569G01N33/577
CPCG01N33/56983G01N33/577
Inventor 刘萍黄爱龙栾大伟张振斌王程飞刘汉昆杨桂霞张佳丽汪德强陈娟
Owner BIOSCIENCE (TIANJIN) DIAGNOSTIC TECH CO LTD