A method for enhancing the contrast of micro-CT plant samples

Abstract

The invention discloses a method for enhancing the contrast of micro-CT plant samples. The method comprises the following steps: 1) dissolving phosphotungstic acid in FAA fixative solution to obtain a phosphotungstic acid solution; 2) using the phosphotungstic acid solution to soak the plant parts to be tested for fixation and dyeing; 3) through the steps 2) The fixed and dyed plant parts are sequentially dehydrated and dried at the critical point of carbon dioxide, which can enhance the contrast of the micro-CT of the plant sample. Micro-CT scanning (Skyscan1172, Bruker Company) was performed on the sample dried at the critical point of carbon dioxide. The scanning voltage can be 41V-59V, and the resolution can be 0.56-1.76μm. , the contrast of plant samples in different parts has been greatly improved, and the contrast of the cytoplasm-rich and protein-rich parts such as magnolia flower buds has been improved more significantly.

Description

technical field [0001] The invention relates to a method for enhancing the contrast of micro-CT plant samples, which belongs to the field of biotechnology. Background technique [0002] Micro-CT technology has been born for more than 40 years, and it has played a huge role in medicine, and has many applications in geology and materials science. In the late 1990s, CT began to be used in plant research to study root structure, morphological development, and plant fossils, but it is rarely found in softer tissues such as young organs, tissues with low or uniform density. Research. [0003] Micro-CT imaging requires X-rays, while X-ray contrast imaging relies on the density, thickness, and atomic number of the sample (Lusic and Grinstaff, 2013). Since plant tissues are mainly composed of light elements, the X-ray absorption rate is low (about 20-40%), and the imaging effect of samples with an absorption rate of about 65-90% is the best. In addition, young plant tissues have n...

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Problems solved by technology

In the late 1990s, CT began to be used in plant research to study root structure, morphological development, and plant fossils, but it is rarely found in softer tissues such as young organs, tissues with low or uniform density. Research

These lead to no significant difference in ray transmittance, and it is very difficult to distinguish the edges of sample structures with similar densities. The reconstructed image has very little contrast and high noise (Rousseau et al., 2015)

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