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Application method of retention index in gas chromatography-tandem mass spectrometry analysis of tobacco flavor components

A technology of retention index and gas chromatography, applied in the field of acquisition time window, can solve problems such as insufficient sensitivity, complex tobacco matrix, sample matrix interference, etc., and achieve the effect of reducing workload

Pending Publication Date: 2020-07-10
ZHENGZHOU TOBACCO RES INST OF CNTC
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the differences in the properties of flavor components and the complexity of the tobacco matrix make the analysis and research of its key flavor components challenging.
At present, gas chromatography-mass spectrometry (GC-MS) combined with general-purpose commercial spectral libraries is mostly used to analyze tobacco aroma components. Due to the constraints of sample matrix interference and insufficient sensitivity of GC-MS, the number of compounds that can be accurately characterized is extremely limited. Previous studies covered less than one hundred flavor components of tobacco leaves
In previous studies, the retention index was mostly used in the GC-MS analysis method to assist in the qualitative analysis of the target components, but the use of the retention index to determine the acquisition time window of the multi-target GC-MS / MS method has not been reported yet.

Method used

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  • Application method of retention index in gas chromatography-tandem mass spectrometry analysis of tobacco flavor components
  • Application method of retention index in gas chromatography-tandem mass spectrometry analysis of tobacco flavor components
  • Application method of retention index in gas chromatography-tandem mass spectrometry analysis of tobacco flavor components

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example 1

[0037] Establishment of the retention index database of 241 kinds of tobacco aroma components.

[0038] The involved tobacco flavor components include aldehydes, ketones, alcohols, phenols, hydrocarbons, ethers, esters, lactones, pyridines, pyrazines and other types of volatile and semi-volatile flavor components. Under programmed temperature conditions, the calculation of the RI of the target compound uses the n-alkane series as a reference, and the calculation formula is:

[0039]

[0040] In the formula: RI is the retention index of the target compound,

[0041] z is the number of carbon atoms of adjacent normal alkanes before the target compound flows out,

[0042] t (x) is the measured retention time of the target compound,

[0043] t (z) is the measured retention time of adjacent n-alkanes before the target compound elutes,

[0044] t (z+1) is the measured retention time of adjacent n-alkanes after the target compound elutes.

[0045] GC-MS / MS analysis conditio...

example 2

[0059] Application of RI in time window correction of dynamic multiple reaction monitoring (dMRM) mode in GC-MS / MS analysis.

[0060] The dMRM mode obtains target information by scanning characteristic ion pairs at the retention time of the target, and its scanning range is a time window created around the exact retention time of each target. In the ultra-multi-target analysis method, a large number of time windows overlap each other and pile up, which will shorten the dwell time of each ion pair, reduce the number of collected chromatographic peak data points, and even lead to the failure of data collection. Therefore, in order to ensure that the chromatographic peaks have enough data points and each ion pair has enough dwell time, the time window is generally reduced as much as possible. For example, the time window of each target in this method is only 0.6min, that is, the instrument only The characteristic ion pairs were scanned within 0.3 min before and after the target r...

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Abstract

The invention relates to an application method of retention index in gas chromatography-tandem mass spectrometry analysis of tobacco flavor components. According to the method, the retention index isused for rapidly determining a collection time window in a gas chromatography-tandem mass spectrometry dynamic multi-reaction monitoring mode (dMRM) analysis method, and the method belongs to the technical field of tobacco component detection and is characterized in that a retention index database of a target compound is firstly established, and the retention time of the target compound can be predicted according to the actually measured retention time of n-alkane series on the basis of acquiring the database so that the time window of each target compound in the dMRM method is accurately andquickly created. The method has the advantages of rapidness, convenience and accuracy, and the workload of retention time correction is greatly reduced.

Description

technical field [0001] The invention belongs to the technical field of detection of tobacco aroma components, and in particular relates to a method for quickly determining the acquisition time window in the analysis method of gas chromatography tandem mass spectrometry (GC-MS / MS) dynamic multiple reaction monitoring mode (dMRM) by using retention index. Background technique [0002] Tobacco aroma components include aldehydes, ketones, alcohols, phenols, acids, ethers, esters, lactones, alkenes, pyridines, pyrroles, pyrazines and other types of volatile and semi-volatile components. The content level and mutual ratio of these components have a key impact on the style and quality of tobacco leaves and cigarettes, and are important chemical components that affect the aroma, quantity and flavor of tobacco. With the advancement of analytical techniques, the influence of aroma components on the sensory quality of tobacco leaves has received more and more attention. However, the d...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/86
CPCG01N30/02G01N30/8644G01N30/8668
Inventor 陈黎任宗灿史天彩刘惠民谢复炜崔华鹏秦亚琼陈满堂刘绍锋樊美娟王晓瑜
Owner ZHENGZHOU TOBACCO RES INST OF CNTC
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