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A kind of chitosan-based cell target adhesion controlled release hydrogel and its preparation method and application

A chitosan and cell-based technology is applied in the field of chitosan-based cell-targeted adhesion and controlled-release hydrogel and its preparation, which can solve the problems of complex modification process and avoid the use and process of toxic chemical reagents. Simple, broad-spectrum effects

Active Publication Date: 2021-05-28
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] However, the above prior art uses chemical methods to introduce chemical groups to achieve the adhesion properties of hydrogel carrier materials that have adhesion to cells or mucous membranes; and the modification reagents used in the chemical method operation process have biological Toxicity, and the modification process is complex; due to the delivery of functional factors in the gastrointestinal tract, considering the biological safety of the hydrogel carrier material, the chemical modification of the carrier material also requires strict separation operations

Method used

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  • A kind of chitosan-based cell target adhesion controlled release hydrogel and its preparation method and application
  • A kind of chitosan-based cell target adhesion controlled release hydrogel and its preparation method and application
  • A kind of chitosan-based cell target adhesion controlled release hydrogel and its preparation method and application

Examples

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Effect test

Embodiment 1

[0049] Example 1 Preparation of chitosan-based cell-targeted adhesion controlled release hydrogel

[0050] A preparation method of chitosan-based cell targeted adhesion controlled release hydrogel, comprising the following steps:

[0051] S1. Preparation of chitosan-based hydrogel film

[0052] Weigh chitosan powder with a molecular weight of 150000g / mol, dissolve it in acetic acid solution, mix it into a chitosan acetate solution with a concentration of 4%, and dry it at 45° C. for 12 hours to obtain a chitosan-based hydrogel film;

[0053] S2. Plasma modified chitosan-based hydrogel film

[0054] The chitosan-based hydrogel film obtained in step S1 is placed in a reaction chamber filled with nitrogen, and connected to high-voltage direct current (voltage 10kV, current 0.25mA) plasma modification treatment for 90min;

[0055] S3. Preparation of chitosan-based cell-targeted adhesion controlled release hydrogel

[0056] After dropping the PBS solution (pH 7.4) containing 18u...

Embodiment 2

[0057] Example 2 Preparation of Chitosan-Based Cell Targeted Adhesion Controlled Release Hydrogel

[0058] A preparation method of chitosan-based cell targeted adhesion controlled release hydrogel, comprising the following steps:

[0059] S1. Preparation of chitosan-based hydrogel film

[0060] Weigh chitosan powder with a molecular weight of 10000g / mol, dissolve it in acetic acid solution, mix it into a chitosan acetate solution with a concentration of 1%, and dry it at 40°C for 11 hours to obtain a chitosan-based hydrogel film;

[0061] S2. Plasma modified chitosan-based hydrogel film

[0062] The chitosan-based hydrogel film obtained in step S1 is placed in a reaction chamber filled with nitrogen, and connected to high-voltage direct current (voltage 10kV, current 0.15mA) plasma modification treatment for 10min;

[0063] S3. Preparation of chitosan-based cell-targeted adhesion controlled release hydrogel

[0064] After dropping the PBS solution (pH 7.4) containing 32ug / m...

Embodiment 3

[0065] Example 3 Preparation of chitosan-based cell-targeted adhesion controlled release hydrogel

[0066] A preparation method of chitosan-based cell targeted adhesion controlled release hydrogel, comprising the following steps:

[0067] S1. Preparation of chitosan-based hydrogel film

[0068] Weigh chitosan powder with a molecular weight of 600,000 g / mol, dissolve it in acetic acid solution, mix it into a chitosan acetate solution with a concentration of 4%, and dry it at 50° C. for 13 hours to obtain a chitosan-based hydrogel film;

[0069] S2. Plasma modified chitosan-based hydrogel film

[0070] The chitosan-based hydrogel film obtained in step S1 is placed in a reaction chamber filled with nitrogen, and connected to high-voltage direct current (voltage 15kV, current 0.5mA) plasma modification treatment for 70min;

[0071] S3. Preparation of chitosan-based cell-targeted adhesion controlled release hydrogel

[0072] After dropping the PBS solution (pH 7.4) containing 8u...

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Abstract

The invention discloses a chitosan-based cell targeted adhesion controlled release hydrogel, a preparation method and application thereof. The invention provides a preparation method of chitosan-based cell targeted adhesion and controlled release hydrogel. After drying the chitosan acetate solution to form a film, perform plasma modification treatment, and then add concanavalin-containing The PBS solution is shaken, passivated, and dried to obtain the chitosan-based cell-targeted adhesion controlled-release hydrogel. The chitosan-based cell-targeted adhesion controlled-release hydrogel has cell-targeted adhesion properties and controlled release properties, which is beneficial to the adhesion and growth of cells; the invention also constructs a non-toxic, safe and controllable hydrogel The controlled release system of active substances significantly improves the absorption and bioavailability of active functional factors in the colon; in addition, the preparation method of the present invention is simple and environmentally friendly. It has broad application prospects in the carrier material of controlled release.

Description

technical field [0001] The invention belongs to the field of biotechnology. More specifically, it relates to a chitosan-based cell targeted adhesion controlled release hydrogel and its preparation method and application. Background technique [0002] Functional foods rich in active functional factors have attracted much attention due to their special physiological regulation. The bioavailability of active functional factors is a key issue for functional foods. In order to solve this problem, the active functional factors are generally embedded with carrier materials (especially natural polysaccharide materials with wide sources, non-toxic and safe), and then the microcapsules are delivered to the site of action to reduce the presence of active functional factors in the human digestive tract. Unnecessary inactivation. [0003] With the development of material science, pharmacy and food science, the transmission of active functional factors has developed from passive transm...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08J3/075C08J7/12C08J5/18C08L5/08C08L89/00A61K9/06A61K9/28A61K47/36A61K47/42
CPCA61K9/06A61K9/2886A61K47/36A61K47/42C08J3/075C08J5/18C08J7/123C08J2305/08C08J2389/00C08J2405/08C08J2489/00
Inventor 司徒文贝黎耀元杨婧雯宋贤良罗树灿张钦发魏韬
Owner SOUTH CHINA AGRI UNIV