Method for rapidly detecting sperm concentration by near infrared spectroscopy

A near-infrared spectroscopy, fast technology, applied in the direction of measuring devices, material analysis through optical means, instruments, etc., can solve the problems of difficult control of experimental errors, difficulty in large-scale promotion, complicated operation process, etc., to eliminate the difference in results, The effect of improving repeatability and reliability

Pending Publication Date: 2020-07-24
刘晓强
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  • Summary
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  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Neubauer's improved hemocytometer method is complicated to operate, and the experimental error is difficult to control, and the results are obtained by manual counting, which must be performed by experienced examiners, and it is difficult to be widely used in clinical practice.

Method used

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  • Method for rapidly detecting sperm concentration by near infrared spectroscopy
  • Method for rapidly detecting sperm concentration by near infrared spectroscopy
  • Method for rapidly detecting sperm concentration by near infrared spectroscopy

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0048] (2) Preparation of semen samples

[0049]Use a pipette gun to stir the liquefied semen evenly, extract about 1uL of semen, and then drop it onto the special slide port that has been treated with static electricity (supporting the measurement of six different samples at the same time), the semen will be sucked into the slide due to the principle of siphon Inside, a homogeneous monolayer of sperm cells will eventually form in the semen sample.

[0050] (3) Light source preheating and connection

[0051] Put the light source (Ocean Optics HL-2000-LL) on the test bench and fix it, turn on the power and preheat it for about 20 minutes, connect the coupling end of the one-point-three optical fiber to the light source, connect the measurement end 1 to the NIRQ512 model spectrometer, and connect the measurement end 2 to the USB2000+ model For the spectrometer, the light-emitting end is connected to the detection device and fixed, and the spectrometer is connected to the PC to ...

Embodiment

[0069] Adopt the inventive method to measure the sperm concentration of 6 patients, concrete measurement method step is as follows:

[0070] (1) Semen liquefaction

[0071] Collect the semen sample in a destaticized and sterile container, and then let it stand at room temperature for about 30 minutes to liquefy the semen naturally.

[0072] (2) Preparation of semen samples

[0073] Use a 10ul pipette gun to stir the liquefied semen evenly, extract about 1uL of semen, and then drop it onto the special glass slide port that has been treated with static electricity (supporting the measurement of six different samples at the same time), the semen will be sucked into the glass due to the principle of siphon Inside the slice, a homogeneous monolayer of sperm cells will eventually form in the semen sample.

[0074] (3) Light source preheating treatment and connection

[0075] Put the light source on the test bench and fix it, turn on the power and preheat it for about 20 minutes, ...

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PUM

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Abstract

The invention discloses a method for quickly detecting sperm concentration by near infrared spectroscopy. The method comprises the following steps of collecting a seminal fluid sample by a test tube,and naturally liquefying seminal fluid at room temperature; sucking the liquefied seminal fluid to a slide subjected to destaticization and sterile treatment, and then putting the slide into a detection device; and connecting a preheated light source with a spectrograph through a one-to-three optical fiber, then fixedly placing the optical fiber at an angle in the detection device to irradiate theslide under different conditions, setting the spectral integration time and the spectral band, and obtaining spectral data of sperms. According to the method, the sperm absorbance is calculated by utilizing the spectral data under the different irradiation conditions, so that the sperm concentration condition is evaluated, the fertility of different male individuals can be effectively evaluated,the individual sperm quality change condition is clear, healthy men are guided to select the optimal same-room opportunity, and the pregnancy possibility is improved.

Description

technical field [0001] The invention belongs to the field of sperm concentration detection methods, and in particular relates to a method for rapidly detecting sperm concentration by near-infrared spectroscopy. Background technique [0002] In recent years, infertility has become a common disease in andrology clinics. At present, infertility is not only a personal problem, but has gradually become a global public social problem, and it is one of the important contents of reproductive health services in many countries. About 20% of infertility in infertile couples is entirely caused by the man, 30%-40% is caused by both men and women, and the man's factor accounts for almost half of the causes of infertility. Clinically, the preferred routine examination for male fertility evaluation is semen routine examination, which mainly uses computer-aided assessment and analysis of sperm concentration, motility, and morphology and other parameters in semen. [0003] Among them, the to...

Claims

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Application Information

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IPC IPC(8): G01N21/359G01N21/01
CPCG01N21/01G01N21/359G01N2021/0112
Inventor 刘晓强欧宁静韩嘉文王慧泉刘莉张威宋宇轩
Owner 刘晓强
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