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Liver cancer circulating tumor cell phenotype analysis method

A technology for tumor cell and phenotype analysis, which is applied in the direction of analysis materials, measuring devices, instruments, etc., can solve the problems of limited clinical application, low capture efficiency, and single capture phenotype, and achieve improved separation efficiency, efficient capture, and improved purity Effect

Inactive Publication Date: 2020-07-24
WISDOM HEALTHY BIOTECH XIAMEN CO LTD
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Problems solved by technology

Many studies have shown that the overexpression of vimentin, a mesenchymal marker, is strongly correlated with advanced hepatocellular carcinoma and poorer prognosis. Therefore, the capture of circulating tumor cells in liver cancer based only on EpCAM recognition will result in low capture efficiency and poor capture performance. The problem of single type and limited clinical application

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  • Liver cancer circulating tumor cell phenotype analysis method
  • Liver cancer circulating tumor cell phenotype analysis method
  • Liver cancer circulating tumor cell phenotype analysis method

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Embodiment Construction

[0040] Please check Figure 1 to Figure 5, a liver cancer circulating tumor cell capture chip, which includes four layers from bottom to top, respectively: glass slide 1, PDMS lower layer 2, PDMS channel layer 3, PDMS upper layer 4.

[0041] Wherein, four sample inlets and four sample outlets are provided on the PDMS upper layer 4, respectively: the first sample inlet 41, the second sample inlet 42, the third sample inlet 43, the fourth sample inlet 44 , the first sample outlet 45 , the second sample outlet 46 , the third sample outlet 47 and the fourth sample outlet 48 .

[0042] On the PDMS channel layer 3, there are two parallel identical processing units, that is, a first processing unit 3A and a second processing unit 3B. in,

[0043] The first processing unit 3A includes: a microarray formed by a plurality of triangular microcolumns 35 in cross section, and a first injection port 41 on the left side of the triangular microarray, a second injection port 42 and a microar...

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Abstract

The invention discloses a liver cancer circulating tumor cell phenotype analysis method. The method comprises the following steps of: 1) constructing a micro-fluidic chip comprising two processing units, wherein each processing unit comprises a micro-array consisting of a plurality of micro-columns with triangular cross sections, two sample inlets positioned on one side of an inlet of the triangular micro-array and two sample outlets positioned on one side of an outlet of the triangular micro-array; the two processing units correspondingly modify epithelial cell adhesion molecule EpCAM antibody and asialoglycoprotein receptor ASGPR antibody 2) samples enter from the inlets of the two processing units correspondingly and flow out from the outlets; and 3) identifying epithelial phenotype andnon-epithelial phenotype of the circulating tumor cells captured by the two processing units through an immunostaining method. According to the method, the capture efficiency, purity and detection rate of the liver cancer circulating tumor cells can be improved, and phenotype analysis can be carried out on the liver cancer circulating tumor cells.

Description

technical field [0001] The invention relates to a method for analyzing the phenotype of circulating tumor cells of liver cancer. Background technique [0002] Hepatocellular carcinoma is the fifth most common cancer in the world and the third leading cause of cancer-related death. The gold standard for the diagnosis of hepatocellular carcinoma is tissue biopsy, but this method has risks such as difficult sampling, incomplete representation, and complications. Early screening of HCC mainly relies on imaging methods and serum alpha fetoprotein (AFP) detection. Imaging methods rely more on the subjective judgment of the operator, and the sensitivity is not enough. The sensitivity and specificity of AFP detection are only 39-64% and 76-91%, respectively, and the false positive and false negative rates are high. Therefore, it is urgent to find a method for the diagnosis of HCC with high accuracy, high sensitivity and non-invasiveness. [0003] Circulating tumor cells (Circula...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/569
CPCG01N33/56966
Inventor 杨朝勇朱琳林慧彬万霜陈小锋宋彦龄
Owner WISDOM HEALTHY BIOTECH XIAMEN CO LTD
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