Sampling liquid and sampling method of DNA viruses in aerosol
A DNA virus and aerosol technology, applied in the field of microorganisms, can solve problems such as inconvenience in research and detection, and achieve the effect of reducing operational risks
Pending Publication Date: 2020-07-28
WUHAN POLYTECHNIC UNIVERSITY
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Problems solved by technology
[0004] The main purpose of the present invention is to propose a sampling method for sampling liquid and DNA virus in aerosol, aiming to solve the problem that the pathogens collected in the sampling liquid in the existing sampling method are still active and inconvenient for subsequent research and detection
Method used
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Embodiment 1
[0059] Sampling solution: 9 mL of the first storage solution, 9 mL of the second storage solution, 600 μL of the third storage solution, 5.5 mL of the fourth storage solution, 0.9 mL of the fifth storage solution and 2 mL of the sixth storage solution.
Embodiment 2
[0061] Sampling solution: 8 mL of the first storage solution, 8 mL of the second storage solution, 400 μL of the third storage solution, 5 mL of the fourth storage solution, 0.8 mL of the fifth storage solution and 1 mL of the sixth storage solution.
Embodiment 3
[0063] Sampling solution: 10 mL of the first storage solution, 10 mL of the second storage solution, 800 μL of the third storage solution, 6 mL of the fourth storage solution, 1 mL of the fifth storage solution and 3 mL of the sixth storage solution.
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Abstract
The invention discloses sampling liquid and a sampling method of DNA viruses in aerosol. The sampling liquid comprises first storing liquid, second storing liquid, third storing liquid, fourth storingliquid, fifth storing liquid, and sixth storing liquid, wherein the first storing liquid is Tris saturated phenol of which the pH is 8.0-8.5, the second storing liquid is a mixed solution of chloroform and isoamyl alcohol in the volume ratio being 24 to 1, the third storing liquid is a beta-mercaptoethanol aqueous solution of which the mass concentration is 0.2-1%, the fourth storing liquid is apolyvinylpyrrolidone aqueous solution of which the mass concentration is 10%, the fifth storing liquid is an ethylenediaminetetraacetic acid aqueous solution of which the molar concentration is 1M, and the sixth storing liquid is a low-melting-point agarose aqueous solution of which the concentration is 19g / L. The sampling liquid provided by the invention can enable the DNA viruses in the aerosolto be inactivated, nucleic acid of the viruses is released to the sampling liquid, and operation risks of researchers in a sampling and detection process can be reduced.
Description
technical field [0001] The invention relates to the technical field of microbes, in particular to a sampling method for sampling liquid and DNA virus in aerosol. Background technique [0002] Aerosol is a dispersed system composed of solid and liquid particles and gaseous medium, while bioaerosol is a solid or liquid particle containing living active substances such as microorganisms or biological macromolecules suspended in the atmosphere. DNA viruses in the atmosphere are usually adsorbed on the surface of particulate matter in the atmosphere, forming bioaerosols. Their types and pathogenicity are closely related to human health, and can cause respiratory infections and other diseases. [0003] The detection of microbial aerosols in the environment includes two important links, one is the sampling link, and the other is the later detection link. At present, the sampling methods of aerosol pathogens include natural sedimentation method, impact method, impact method, centri...
Claims
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IPC IPC(8): C12N15/10
CPCC12N15/1003
Inventor 梅运军董文华张磊杨奕
Owner WUHAN POLYTECHNIC UNIVERSITY