External common-optical-path off-axis digital holographic microimaging device

A technology of digital holographic microscopy and imaging devices, which is applied in measuring devices, material analysis through optical means, instruments, etc., can solve problems such as high coherent noise and difficult optical path adjustment, and achieve high coherent noise and difficult optical path adjustment , High dynamic real-time measurement effect

Inactive Publication Date: 2020-07-28
BEIJING INSTITUTE OF TECHNOLOGYGY
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Problems solved by technology

[0004] The present invention proposes an external common optical path off-axis digital holographic microscopic imaging device, which can be used for quantitative phase detection of tiny objects such as biological cells/tissues. The present invention solves the problem that existing optical microscopes can only obtain two-dime

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  • External common-optical-path off-axis digital holographic microimaging device
  • External common-optical-path off-axis digital holographic microimaging device
  • External common-optical-path off-axis digital holographic microimaging device

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specific Embodiment

[0031] Specific embodiment: carry out actual test with microlens array as measured object; Commercial optical microscope 14 adopts the optical microscope 37XF that Shanghai optical instrument factory produces; The focal length of the 4th lens 10 is 60mm; The line logarithm of one-dimensional periodic grating 9 is 100Line / mm. The filter 11 is processed by a metal sheet; the imaging detector 12 adopts a CCD camera with a pixel size of 2.2 microns. In actual measurement, the off-axis holograms of the microlens array and a single lens recorded by the imaging detector 12 are shown as Figure 4 (a) and Figure 5 (a) shown. Finally, the off-axis hologram is reconstructed using the well-known numerical reconstruction method in the field of digital holography, and the three-dimensional height information of the measured object can be obtained (such as Figure 4 (b) and Figure 5 (b)), two-dimensional height information (such as Figure 4 (c) and Figure 5 (c)), and the two-dimens...

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Abstract

The invention relates to an external common-optical-path off-axis digital holographic microimaging device. The device sequentially comprises a one-dimensional periodic grating, a fourth lens, a filterand an imaging detector in the optical path direction. Parallel light beams carrying object information are diffracted after passing through the one-dimensional periodic grating, and on the Fourier spectrum surface of the fourth lens, zero-level diffracted light and + 1-level diffracted light filtered by a filter are adopted to generate an off-axis hologram on the target surface of the imaging detector, and the off-axis hologram is recorded by the imaging detector. The off-axis hologram is reconstructed by adopting a well-known numerical reconstruction algorithm in the field of digital holography to obtain the phase information of the measured object. The device is simple and compact in structure and convenient to install and adjust, can be directly used as external equipment of a commercial optical microscope to carry out quantitative microscopic imaging on micro-nano structure objects such as biological cells, and has a very strong market application prospect.

Description

technical field [0001] The invention belongs to the field of digital holographic microscopic imaging, in particular to an external common optical path off-axis digital holographic microscopic imaging device. Background technique [0002] Biological cells / tissues have three-dimensional distribution characteristics, and three-dimensional measurement is a necessary condition for understanding all information about tissue structure and activities. However, most of the cells / tissues in the culture process are unstained original cells / tissues, showing transparent or translucent properties. After the light passes through, only the phase changes, and the wavelength and light intensity (amplitude) are almost unchanged. When observing cells / tissues with a traditional optical microscope, only light intensity (two-dimensional) information can be seen, and phase (three-dimensional) information of cells / tissues cannot be obtained. Therefore, it is the goal pursued by the biomedical field...

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Application Information

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IPC IPC(8): G03H1/00G03H1/04G03H1/08G01N21/84G01N21/01
CPCG01N21/01G01N21/84G01N2021/0112G03H1/0005G03H1/0443G03H1/0866G03H2001/005G03H2001/0445
Inventor 文永富陈欣程灏波
Owner BEIJING INSTITUTE OF TECHNOLOGYGY
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