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Method for preparing zeaxanthin dipalmitate high internal phase emulsion from protein amyloid fiber-polysaccharide complex

A technology of high internal phase emulsion and zeaxanthin, which is applied in the field of preparation of high internal phase emulsion of zeaxanthin dipalmitate, can solve the problems of limited application, low bioavailability, and difficulty in cell absorption, and achieve the effect of improving stability

Pending Publication Date: 2020-07-31
WOLFBERRY ENG RES INST NINGXIA ACADEMY OF AGRI & FORESTRY SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, until now, because the molecular structure of zeaxanthin dipalmitate contains unsaturated double bonds, it is easily oxidized and decomposed by oxygen, light, and heat in the air, and it is a water-insoluble substance, which is difficult to absorb by cells in the body and has low bioavailability. low, which greatly limits its application
[0004] There is no technology and method for preparing zeaxanthin dipalmitate high internal phase emulsion by using amyloid fiber-polysaccharide complex

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Embodiment one: a kind of method that utilizes protein amyloid fiber-polysaccharide complex to prepare zeaxanthin dipalmitate high internal phase emulsion, the method is specifically as follows:

[0017] (1) Heat egg white lysozyme (HEWL) at pH 2, 90°C for 8 hours to prepare HEWL amyloid fiber solution;

[0018] (2) Mix seaweed polysaccharides with a pH of 2 to 6 and a concentration of 0.002 to 0.01 wt% and 0.025 to 0.5 wt% HEWL amyloid fiber solution of the corresponding pH at a ratio of 1:1 to produce an electrostatic bond to form a HEWL amyloid fiber-polysaccharide composite High Internal Phase Emulsions (HIPEs);

[0019] (3) dissolving zeaxanthin dipalmitate with a concentration of 0.01 to 0.05% in dodecane;

[0020] (4) Dodecane dissolved with zeaxanthin dipalmitate is mixed with HEWL amyloid fiber-polysaccharide complex at a ratio of 75 to 85%, and after high-speed dispersion, the loaded zeaxanthin dipalmitate with an oil phase higher than 74% is obtained Esters...

Embodiment 2

[0021] Embodiment two: a kind of method that utilizes protein amyloid fiber-polysaccharide complex to prepare zeaxanthin dipalmitate high internal phase emulsion, the method is specifically as follows:

[0022] (1) Heat egg white lysozyme (HEWL) at pH 2, 90°C for 8 hours to make HEWL amyloid fibers;

[0023] (2) Mix 0.002-0.01wt% low-methylated pectin with a pH of 2-6 and a concentration of 0.002-0.01wt% with a corresponding pH of 0.025-0.5wt% HEWL amyloid fiber solution to produce electrostatic binding to produce HEWL amyloid Fiber-polysaccharide complex high internal phase emulsions (HIPEs);

[0024] (3) dissolving zeaxanthin dipalmitate with a concentration of 0.01 to 0.05% in dodecane;

[0025] (4) Dodecane dissolved with zeaxanthin dipalmitate is mixed with HEWL amyloid fiber-polysaccharide complex at a ratio of 75 to 85%, and after high-speed dispersion, the loaded zeaxanthin dipalmitate with an oil phase higher than 74% is obtained Esters of HIPEs.

Embodiment 3

[0026] Embodiment three: a kind of method that utilizes protein amyloid fiber-polysaccharide complex to prepare zeaxanthin dipalmitate high internal phase emulsion, the method is specifically as follows:

[0027] (1) Heat egg white lysozyme (HEWL) at pH 2, 90°C for 8 hours to make HEWL amyloid fibers;

[0028] (2) Mix 0.002-0.01wt% hypermethylated pectin with a pH of 2-6 and a concentration of 0.002-0.01wt% with a corresponding pH of 0.025-0.5wt% HEWL amyloid fiber solution to generate electrostatic binding to produce HEWL amyloid Fiber-polysaccharide complex high internal phase emulsions (HIPEs);

[0029] (3) dissolving zeaxanthin dipalmitate with a concentration of 0.01 to 0.05% in dodecane;

[0030] (4) Dodecane dissolved with zeaxanthin dipalmitate is mixed with HEWL amyloid fiber-polysaccharide complex at a ratio of 75 to 85%, and after high-speed dispersion, the loaded zeaxanthin dipalmitate with an oil phase higher than 74% is obtained Esters of HIPEs.

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Abstract

The invention relates to a preparation method of a zeaxanthin dipalmitate high internal phase emulsion, in particular to a method for preparing the zeaxanthin dipalmitate high internal phase emulsionfrom a protein amyloid fiber-polysaccharide complex. The method comprises the following steps: firstly, preparing an amyloid fiber solution, electrostatically binding the amyloid fiber solution with polysaccharide to prepare an amyloid fiber-polysaccharide complex high internal phase emulsion, then, dissolving zeaxanthin dipalmitate with a concentration of 0.01-0.05wt% in dodecane, mixing the dodecane with the zeaxanthin dipalmitate dissolved with the amyloid fiber-polysaccharide complex in a volume ratio being 75-85%, and performing high-speed dispersion to prepare zeaxanthin dipalmitate-loaded high internal phase emulsion with an oil phase higher than 74%. The method is unique, can improve the stability and bioavailability of the zeaxanthin dipalmitate, and lays a foundation for furtherresearch and development of effect functions and mechanisms.

Description

technical field [0001] The invention relates to a preparation method of a zeaxanthin dipalmitate high internal phase emulsion, in particular to a method for preparing a zeaxanthin dipalmitate high internal phase emulsion by utilizing a protein amyloid fiber-polysaccharide compound. Background technique [0002] Lycium barbarum is a precious Chinese herbal medicine and tonic, rich in functional substances such as carotenoids. Zeaxanthin dipalmitate is a characteristic carotenoid in wolfberry, and its content can reach 50% to 75% of the total carotenoids in wolfberry. [0003] Zeaxanthin dipalmitate (also known as Physalis red pigment, (3R,3'R)-3,3'-Dihexadecanoyloxy-β,β-carotene, CAS number: 144-67-2, molecular formula C 72 h 116 o 4 ) is a fat-soluble polyisoprene compound, and it has been confirmed that zeaxanthin dipalmitic acid can alleviate the inflammatory response and oxidative stress injury of liver Kupffer cells caused by carbon tetrachloride in animal models, as ...

Claims

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Application Information

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IPC IPC(8): A61K31/23A61K9/107A61K47/36A61P27/02A61P27/12A61P1/16
CPCA61K31/23A61K9/107A61K47/36A61P27/02A61P27/12A61P1/16
Inventor 闫亚美米佳曹有龙罗青禄璐李晓莺李越鲲冉林武张曦燕安巍
Owner WOLFBERRY ENG RES INST NINGXIA ACADEMY OF AGRI & FORESTRY SCI