Rhodococcus ruber HDRR1 for purifying inorganic nitrogen and phosphorus in aquaculture tail water of seawater ponds and applications of rhodococcus ruber HDRR1

A technology of Rhodococcus rubrum and inorganic nitrogen, applied in Rhodococcus rubrum HDRR1 and its application fields, can solve the problem of difficult separation and purification of nitrifying bacteria and denitrifying bacteria, small-scale retention, and large-scale industrial application effect of directional cultivation of single microorganisms, etc. problems, achieve good environmental adaptability, reduce water body replacement, and achieve remarkable removal effects

Active Publication Date: 2020-07-31
SHENZHEN BASE OF SOUTH CHINA SEA FISHERIES RES INST CHINESE ACAD OF FISHERY SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Because nitrifying bacteria and denitrifying bacteria are relatively difficult to separate and purify, most researchers at home and abroad use activated sludge for enrichment culture, and seldom use pure bacteria to expand the culture method, and form pure bacteria that can be used in aquaculture industry on a large scale Preparation products are rarely reported (Yang Ni

Method used

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  • Rhodococcus ruber HDRR1 for purifying inorganic nitrogen and phosphorus in aquaculture tail water of seawater ponds and applications of rhodococcus ruber HDRR1
  • Rhodococcus ruber HDRR1 for purifying inorganic nitrogen and phosphorus in aquaculture tail water of seawater ponds and applications of rhodococcus ruber HDRR1
  • Rhodococcus ruber HDRR1 for purifying inorganic nitrogen and phosphorus in aquaculture tail water of seawater ponds and applications of rhodococcus ruber HDRR1

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Example 1 Screening and cultivation of Rhodococcus rhodococcus HDRR1 for purification of inorganic nitrogen and phosphorus in seawater pond culture tail water

[0030] 1. Material preparation

[0031] 1.1. Bacterial source

[0032] In the intensive prawn culture pond in Pinghai Town, Huidong County, Guangdong Province, the tail water samples from 60-90 days of culture were collected, and the selective medium plate was used for separation and culture.

[0033] 1.2. Medium

[0034] (1) Selective liquid medium: CH 3 COONa: 1g, yeast extract: 1g, MgSO 4 ·7H 2 O: 0.4g, NaCl: 0.1g, CaCl 2 2H 2 O: 0.05g, NaHCO 3 : 0.3g, KH 2 PO 4 : 1g, 1mL of trace element solution, the above drugs were dissolved in distilled water, and fixed to 1000mL, pH7.0.

[0035] Trace element solution: EDTA: 2.5g, ZnSO 4 ·7H 2 O: 10.95g, MnSO 4 ·H 2 O: 1.54g, CuS0 4 ·5H 2 O: 0.39g, CoCl 2 ·6H 2 O: 0.2g, FeSO 4 ·7H 2 O: 7g, glutamic acid: 0.02g, the above drugs were respectively disso...

Embodiment 2

[0041] Example 2 Identification of the screened Rhodococcus rubrum HDRR1

[0042] In the present invention, the 16S rDNA molecular identification of the screened Rhodococcus rubrum HDRR1 is carried out, and the species of the strain is determined from the molecular level, combined with the analysis of the morphological characteristics of the bacteria and the physiological and biochemical characteristics. 16S rDNA sequence analysis mainly follows the following steps:

[0043] 1. Extraction of bacterial genomic DNA:

[0044] (1) Use a sterile toothpick to pick a single colony and inoculate it in the expansion medium for cultivation;

[0045] (2) Take 1.5 mL of bacterial culture solution, centrifuge at 10,000 rpm (11,500×g) for 1 minute, and suck up the supernatant as much as possible;

[0046] (3) Add 200 μL of buffer GA to the cell pellet, shake until the cell is completely suspended, add 180 μL of lysozyme with a final concentration of 20 mg / mL, and treat at 37°C for more th...

Embodiment 3

[0068] Example 3 Removal effect of Rhodococcus erythrococcus HDRR1 on inorganic nitrogen and phosphorus in seawater pond culture tail water

[0069] 1. Growth of the strain

[0070] The bacterial strain Rhodococcus rubrum HDRR1 that embodiment 1 obtains presses initial concentration 10 4 ~10 6 CFU / mL is inoculated into the tail water of sterilized intensive culture ponds, and the bacterial count can increase to 10 in about 2 days. 8 CFU / mL, and the bacterial count remained stable at 10 within 3 to 10 days. 8 CFU / mL quantity level, the growth curve of Rhodococcus rhodococcus HDRR1 is as follows figure 1 shown in .

[0071] 2. Inorganic nitrogen and phosphorus removal effect of strains in different salinity aquaculture tail waters

[0072] The tail water of the sterilized shrimp intensive culture pond (water salinity 25) was used as the basic test water control, and Rhodococcus rubrum HDRR1 was not added during the test. The bacterium-added group adjusts the salinity of th...

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Abstract

The invention discloses rhodococcus ruber HDRR1 for purifying inorganic nitrogen and phosphorus in aquaculture tail water of seawater ponds. The rhodococcus ruber HDRR1 is assigned the accession number CCTCC M20191008, and deposited with the China Center for Type Culture Collection, Wuhan, China on December 4, 2019. The strain HDRR1 has strong purifying capacity on the inorganic nitrogen and phosphorus in aquaculture tail water, is good in environmental adaptability and has no adverse effects on farmed fish and shrimp. Applications of the rhodococcus ruber HDRR1 on purifying the inorganic nitrogen and phosphorus in the aquaculture tail water of seawater ponds are also disclosed.

Description

technical field [0001] The invention belongs to the technical field of microbial purification of seawater pond culture water, in particular to a Rhodococcus rubrum HDRR1 for purifying inorganic nitrogen and phosphorus in seawater pond culture tail water and an application thereof. Background technique [0002] In 2018, the total output of aquaculture in my country exceeded 50 million tons, accounting for more than 78% of the total supply of aquatic products. If the aquaculture process is not properly managed, residual feed, aquaculture metabolites and aquatic biological residues will accumulate in the water environment, forming self-sourced aquaculture tail water or pollutants. In terms of the form of chemical substances contained in the tail water, there are mainly ammonia nitrogen, total inorganic nitrogen (TIN), phosphorus, organic matter and fouling organisms (Xu Sheng, 2011; Shu Tingfei et al., 2002). For example, harmful nitrogen such as ammonia nitrogen and nitrite n...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/34C12R1/01C02F103/20C02F101/10C02F101/16
CPCC02F3/34C02F2103/20C02F2101/105C02F2101/16C12R2001/01C12N1/205
Inventor 曹煜成胡晓娟文国樑徐煜苏浩昌徐武杰杨铿许云娜虞为
Owner SHENZHEN BASE OF SOUTH CHINA SEA FISHERIES RES INST CHINESE ACAD OF FISHERY SCI
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