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Method for detecting content of formyl peptide or formyl peptide receptor-1 ligand, and application thereof

A formyl peptide and ligand technology, applied in the field of biomedicine

Active Publication Date: 2020-07-31
GENERAL HOSPITAL OF TIANJIN MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] However, at present, there is a lack of methods to assess the progression of cerebral edema after intracerebral hemorrhage by examining the content of mitochondrial formyl peptide in the blood.

Method used

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  • Method for detecting content of formyl peptide or formyl peptide receptor-1 ligand, and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] Example 1: CT image and volume assessment of PHE and hematoma

[0050] Patients with cerebral hemorrhage were enrolled, and the fourth-generation CT scanner (Somatom 64 or Somatom AS+, Siemens Healthcare, Erlangen) was used for cranial CT tomography. Each scan contained a multi-slice spiral CT data set (1-24 slices, slice thickness 5mm ). Draw the injury area and hematoma outline on each slice of the head CT image, use MRIcron software (Chris Rorden's MRIcron, version 4AUG2014) to calculate the volume of the injury area and hematoma, and use the formula to calculate the volume of perihematoma edema (PHE) : PHE volume = lesion area volume - hematoma volume. In order to evaluate the PHE volume more accurately, we counted the 24-hour CT scan images of cerebral hemorrhage, and recorded the PHE volume and hematoma volume of patients with cerebral hemorrhage. We collected blood samples 24 hours after cerebral hemorrhage from these cerebral hemorrhage patients (see Example...

Embodiment 2

[0051] Example 2: Quantitative detection of plasma mitochondrial N-formylphthalein ELISA

[0052] Human methionine (fMET) ELISA kit was used to detect the content of plasma mitochondrial N-formylphthalein according to the finished product instructions. Plasma samples were diluted 1:5 with the sample diluent in the kit before adding to the ELISA plate.

[0053] 2.1 Sample collection and storage

[0054] Plasma—Samples are collected in collection tubes containing anticoagulant EDTA or heparin, and centrifuged at 1000g for 15 minutes at 2-8°C within 30 minutes after sample collection. Collect the supernatant and assay immediately, or store supernatant samples in batches at -20°C or -80°C for later use. Avoid repeated freezing and thawing of samples. Note: Samples should be centrifuged sufficiently to avoid hemolysis or the presence of particles.

[0055] 2.2 Preparations

[0056] Bring all reagents to room temperature before use. If crystals form in the concentrated buffe...

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PUM

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Abstract

The invention relates to a method for detecting the content of a formyl peptide or a formyl peptide receptor-1 ligand, and an application thereof, and concretely relates to an application of a reagentfor detecting the FPR1 ligand in the preparation of a reagent or a kit for detecting acute brain injury or encephaledema change, and a method for evaluating and predicting acute brain injury such asencephaledema change after cerebral hemorrhage. By means of the application and the method, it can be determined that the level of the N-formylphthalide or FPR1 ligand in peripheral blood is positively related to the progress of hematoma surrounding edema after cerebral hemorrhage, the N-formyl phthalide or FPR1 ligand is used for evaluating and predicting acute brain injuries such as hematoma surrounding encephaledema changes after cerebral hemorrhage, and a biomarker basis is provided for judging brain injury evolution.

Description

technical field [0001] The present disclosure relates to the field of biomedicine. Specifically, it involves the detection of FPR1 ligands, such as related reagents and technologies for detecting the content of N-formylphthalein or FPR1 ligands in blood, for evaluating and predicting changes in cerebral edema around hematoma after acute brain injury such as cerebral hemorrhage, Provide a biomarker basis for assessing the evolution of brain injury. Background technique [0002] Danger signal molecules (Damage-associated molecular patterns, DAMPs) are a type of endogenous molecules released by dead cells after tissue damage, which activate the immune response after binding to the corresponding ligands expressed by the cells, and activate tissue innate immune cells and mobilize after tissue damage. Key signal for migration of peripheral immune cells to sites of injury. N-formylpeptides (NFPs) are mitochondria-derived DAMPs. Dead cells release NFPs into the extracellular matri...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68G01N33/577
CPCG01N33/577G01N33/6893G01N2333/47G01N2800/28
Inventor 李治国施福东李裕琳韩金蕊么阳
Owner GENERAL HOSPITAL OF TIANJIN MEDICAL UNIV
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