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Application of radix astragali and radix ophiopogonis blood circulation promoting drink in preparation of microRNA-155 inhibitor

An inhibitor, Ophiopogon japonicus technology, applied in the development of new uses of old drugs, the application of Qidong Huoxue Decoction in the preparation of microRNA-155 inhibitors, can solve problems such as abnormal expression, and achieve the effect of reducing inflammatory response

Inactive Publication Date: 2020-08-04
ZHEJIANG CHINESE MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no literature report that Qidong Huoxue Decoction is associated with the abnormal expression of miRNA-155 in the treatment of ALI.

Method used

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  • Application of radix astragali and radix ophiopogonis blood circulation promoting drink in preparation of microRNA-155 inhibitor
  • Application of radix astragali and radix ophiopogonis blood circulation promoting drink in preparation of microRNA-155 inhibitor
  • Application of radix astragali and radix ophiopogonis blood circulation promoting drink in preparation of microRNA-155 inhibitor

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] The prescription and preparation method of embodiment 1 Qidong Huoxue Decoction

[0032] The traditional Chinese medicine composition Qidong Huoxue Yin involved in the present invention has the following prescription composition: astragalus 20g, Radix Ophiopogon japonicus 12g, Polygonum cuspidatum 20g, Angelica sinensis 12g.

[0033] The preparation method of the decoction of Qidong Huoxue Decoction is as follows: take the prescribed amount of raw materials (the raw materials are all purchased from Huadong Medicine Co., Ltd.) The knotweed and angelica are fried together. After boiling with strong fire, fry with slow fire for 30 minutes. After the second fry, add 3-5 times of pure water to the remaining medicinal residue and fry again for 25 minutes. , 4g / ml water decoction (that is, each milliliter contains crude drug 1g, 4g), bottled and capped after subpackaging, and stored at 4°C for future use.

[0034] The concentration of Qidong Huoxue Decoction in mice is determ...

Embodiment 2

[0035] Example 2 Effect of Qidong Huoxue Decoction on expression of miR-155 in alveolar macrophages with acute lung injury

[0036] Mouse-derived RAW264.7 macrophages were purchased from the Shanghai Cell Bank of the Chinese Academy of Sciences. Mouse macrophages RAW264.7 were inoculated in DMEM containing 10% FBS, 1000U / ml penicillin, 100U / ml streptomycin, and 2mM glutamine In the culture medium, it is routinely cultured in an incubator at 37°C, 5% CO2, and saturated humidity. The culture medium is replaced every 1-2 days, and subculture is carried out at a cell density of about 80%. Divided into blank group (normal culture group), model group (LPS induction group), low dose Qidong Huoxue Decoction treatment group (5% drug serum culture), high dose Qidong Huoxue Decoction treatment group (10% drug serum culture ), the dexamethasone group.

[0037] Before the mouse RAW264.7 cell model was established, the culture medium containing 10% FBS was replaced with the DMEM medium con...

Embodiment 3

[0051] Example 3 investigates the effects of miR-155 overexpression and inhibited expression on inflammatory response

[0052] In the same way as in Example 2, an LPS-induced macrophage model was established, and RAW264.7 cells were 6 The concentration of each / ml was inoculated in a 6-well plate and divided into the following groups: blank group, model group (i.e. figure 2 LPS in the treatment group), Qidong Huoxue decoction high dose 4g / ml treatment group (i.e. figure 2 QDHUX in), miR-155mimic transfection group (ie figure 2 miR-155m in), miR-155 mimic NC group (ie figure 2 miR-155m-NC in the model group+miR-155 inhibitor group (ie figure 2 miR-155 in i), model group + miR-155inhibitor NC group (ie figure 2 miR-155 in i-NC). Wherein, the transfection was carried out according to the instructions of the reagents; miR-155 inhibitor is a commercially available known miR-155 inhibitor, which was used as a positive control drug in this example to evaluate whether Qidong...

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Abstract

The invention discloses application of radix astragali and radix ophiopogonis blood circulation promoting drink in preparation of a microRNA-155 inhibitor. The radix astragali and radix ophiopogonis blood circulation promoting drink is prepared from radix astragali, radix ophiopogonis, rhizoma polygoni cuspidati and radix angelicae sinensis. Through the research on the relationship between expression inhibition of miR-155 and inflammatory factors and SOCS1 protein, it is originally found that the radix astragali and radix ophiopogonis blood circulation promoting drink can reduce inflammatory response in acute lung injury macrophages and up-regulate expression of SOCS1 by inhibiting expression of microRNA-155, and therefore the purpose of treating or preventing diseases such as acute lung injury and autoimmune diseases is achieved.

Description

technical field [0001] The technical field of traditional Chinese medicine preparations of the present invention specifically relates to the application of Qidong Huoxue Decoction in the preparation of microRNA-155 inhibitors, which is the development of new uses of old medicines. Background technique [0002] Acute lung injury (ALI) is a syndrome characterized by acute non-cardiogenic pulmonary edema and refractory hypoxemia with uncontrolled inflammatory response as the underlying cause. The main pathological features are: lung tissue A large number of neutrophils accumulate, alveolar epithelial cells and capillary endothelial cells are damaged, and alveolar-capillary membrane permeability increases to form extensive pulmonary edema [1] . [0003] The pathogenesis of ALI in traditional Chinese medicine belongs to the categories of "lung heat syndrome", "chest knot", and "asthma syndrome". Air stagnation, upper reversal leads to panting, middle resistance leads to fullnes...

Claims

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Application Information

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IPC IPC(8): A61K36/8968A61P11/00A61P35/00A61P37/02
CPCA61K36/232A61K36/481A61K36/704A61K36/8968A61P11/00A61P35/00A61P37/02A61K2300/00
Inventor 蔡宛如
Owner ZHEJIANG CHINESE MEDICAL UNIVERSITY
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