Biochemical index detection integrated chip as well as detection method and application thereof

A technology of integrated chips and biochemical indicators, applied in the field of analysis and detection, can solve the problems of reducing the scope and efficiency of diagnosis and treatment, simultaneous detection of biochemical items, and easy failure of reference electricity, etc., so as to reduce the use of detection samples and patients' medical treatment cost, avoid duplication of testing, effect of low blood sample volume

Active Publication Date: 2020-08-07
SUZHOU MNCHIP TECH CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] For this reason, the problem of the technology that the present invention solves is: the blood gas analysis system that mostly adopts clinically at present mainly has the following disadvantages: (1) the blood gas analysis volume used clinically is large, can only be detected in a fixed detection place, and has poor mobility , greatly reducing the scope and efficiency of diagnosis and treatment; (2) professional inspectors are required to operate and strictly carry out daily maintenance work; (3) the results are easily affected by the protein in the blood sample, and protein removal and membrane replacement are required; (4) The electrodes are easy to age, and the reference electrode is prone to failure, which needs to be replaced regularly, and the cost is high; (5) The blood gas analysis system is an equipment system independent of the biochemical analyzer, which cannot be tested simultaneously with most other biochemical items. Increased use of blood samples for testing

Method used

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  • Biochemical index detection integrated chip as well as detection method and application thereof
  • Biochemical index detection integrated chip as well as detection method and application thereof
  • Biochemical index detection integrated chip as well as detection method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0073] Embodiment 1: Detection of blood pH and blood ammonia

[0074] Such as Figure 1a , shown in 2, the chip includes an upper layer 1, a lower layer 2, the upper layer is connected to the lower layer watertightly, the upper layer 1 is provided with a sample through hole 11, and the lower layer 2 includes a sample tank 21, a liquid storage tank 23, and a sample waste liquid tank 25, The sample quantification tank 28 and the micro-reflection surface 217 are connected by a flow channel between each tank; the sample waste liquid tank 25 includes a first waste liquid tank 251 and a second waste liquid tank 252, and the first waste liquid tank 251 is The spherical freeze-dried reagent of the phenol red freeze-dried solution used for measuring pH is installed, and the freeze-dried reaction reagent used for measuring blood ammonia is pre-installed in the second waste liquid tank 252 .

[0075] In the detection operation using the chip, take a human blood serum sample, and then inj...

Embodiment 2

[0076] Example 2: Indicating Whole Blood Samples

[0077] Such as Figure 1a , shown in 2, the chip includes an upper layer 1, a lower layer 2, the upper layer is connected to the lower layer watertightly, the upper layer 1 is provided with a sample through hole 11, and the lower layer 2 includes a sample tank 21, a liquid storage tank 23, and a sample waste liquid tank 25, The sample quantification tank 28 and the micro-reflection surface 217 are connected by a flow channel between each tank; the sample waste liquid tank 25 includes a first waste liquid tank 251 and a second waste liquid tank 252, and the first waste liquid tank 251 is Spherical lyophilized reagent filled with a lyophilized solution of phenol red for pH measurement.

[0078] Wherein, the depth of the first waste liquid tank is 0.5mm, the length of the straight-through channel is 10mm, and the depth is 0.5mm.

[0079]In the detection operation using the chip, the whole blood sample of human blood is taken, an...

Embodiment 3

[0080] Embodiment 3: blood biochemical detection

[0081] As shown in Figures 1 and 2, the chip of the present invention uses transparent plastic as a raw material and is made by injection molding.

[0082] The chip includes an upper layer 1 and a lower layer 2. The upper layer 1 is provided with a sample through hole 11 and a diluent through hole 12. The lower layer 2 is provided with a sample tank 21, a diluent tank 22, a liquid storage tank 23, a reaction detection tank 24, and a sample waste liquid. Groove 25, overflow tank 26, diluent quantitative tank 27, sample quantitative tank 28, mixing tank 29, transition tank 31 and micro-reflection surface 217, are connected by flow channel between described each tank; Sample size indicator structure 219 .

[0083] The sample waste liquid tank 25 includes a first waste liquid tank 251 and a second waste liquid tank 252, the first waste liquid tank and the second waste liquid tank can be used for the detection of sample indicators...

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Abstract

The invention relates to a biochemical index detection integrated chip comprising an upper layer and a lower layer; the upper layer and the lower layer are connected in a water tightness manner, the upper layer is provided with a sample through hole, the lower layer comprises a sample tank, a liquid storage tank, a sample waste liquid tank, a sample quantification tank and a micro-reflection surface, and the tanks are connected through flow channels; the sample waste liquid tank is characterized in that the sample waste liquid tank comprises a first waste liquid tank and / or a second waste liquid tank or two or more waste liquid tanks, and freeze-drying reaction reagents are preloaded in the first waste liquid tank and / or the second waste liquid tank or two or more waste liquid tanks. Whenthe chip is used for detecting the pH value, the cost and the consumption of biological samples and reagents can be greatly reduced, and meanwhile, the defect that the pH value cannot be detected through micro-fluidic in-vitro rapid diagnosis is overcome.

Description

technical field [0001] The invention is used in the field of analysis and detection, and specifically relates to a multifunctional multi-indicator detection integrated chip integrating sample pretreatment, quantitative dilution, reaction and detection, and a detection method and application thereof. Background technique [0002] Facing challenges in the fields of biomedical analysis, disease diagnosis, environmental monitoring, food and drug safety in the 21st century, the requirements for on-site sampling analysis, rapid detection, and patient self-testing have put forward higher requirements for inspection and quarantine analysis methods and equipment. requirements. To meet these new demands, it is necessary to develop miniaturized, integrated and portable inspection and quarantine equipment. [0003] At present, the automated analysis equipment used in inspection and quarantine has been integrated and automated after development. For example, in the automatic biochemica...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01L3/00G01N21/80
CPCB01L3/5027G01N21/80
Inventor 王战会王树相张天乐侯旭彤
Owner SUZHOU MNCHIP TECH CO LTD
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