Method for preparing peanut oil based on fatty acid demulsification
A peanut oil and fatty acid technology, which is applied in the production of fat oil/fat, refining of fat oil/fat, and fat production, can solve the problems of solvent residue in solvent method, destruction of nutrient bioactive substances, etc., and achieves a wide range of application fields, low cost, high The effect of nutritional value
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Embodiment 1
[0044] This embodiment is the preparation embodiment of peanut oil, and its technological process is as follows figure 1 shown, including:
[0045] (A) Peeled peanuts are used for crushing: in this step, the peeled peanuts may be optionally roasted, and the average particle size of the crushed peanuts is 30 μm-50 μm.
[0046] (B) Add water (or water and enzyme) to crushed peanuts and stir to obtain a mixed oil and protein extract: in this step, the weight ratio of crushed peanuts to water is 1:2~5; the enzyme activity is 100-5000U / g (mL), the enzyme dosage is 20-100U / g peanut; the stirring operation can be homogeneous operation, and the solution after adding water and enzyme needs to be fully enzymatically hydrolyzed and inactivated.
[0047] (C) Centrifuge and stratify the mixed extract, carry out corresponding follow-up treatment on the lower layer of precipitation, obtain protein after the middle layer of water phase is dried, and add acid (fatty acid) to the upper layer o...
Embodiment 2
[0050] In this embodiment, hexanoic acid is used to demulsify the oil body emulsion, which specifically includes the following steps:
[0051] (1) Grinding: Grinding 20.00±1.00g of peeled peanuts with a grinder until the average particle size is 40 μm;
[0052] (2) After crushing, mix peanuts and deionized water at a mass ratio of 1:3.5, homogenize at 9000r / min for 2s;
[0053] (3) Transfer the homogenized sample solution to a 400ml centrifuge cup with stopper and centrifuge at 4000r / min for 20min, collect the upper oil body, and store it in a 4°C refrigerator for later use;
[0054] (4) Take 5g of oil body, mix the oil body and deionized water at a mass ratio of 1:4, add a certain volume fraction of hexanoic acid (C6), and stir on a magnetic stirrer at room temperature for 10 minutes;
[0055] (5) Take the stirred emulsion, put it in a 50ml centrifuge tube and centrifuge at 4000r / min for 15min, and the suspended matter in the upper layer is peanut oil. The relationship betw...
Embodiment 3
[0057] In this embodiment, octanoic acid is used to demulsify the oil body emulsion, which specifically includes the following steps:
[0058] (1) Grinding: Grinding 20.00±1.00g of peeled peanuts with a grinder until the average particle size is 40 μm;
[0059] (2) After crushing, mix peanuts and deionized water at a mass ratio of 1:3.5, 75U / g peanut compound plant hydrolase enzyme preparation, homogenize at 9000r / min for 2s;
[0060] (3) Shake the mixed solution obtained in step (2) in a 50°C water bath constant temperature oscillator for 90 minutes to fully enzymatically hydrolyze;
[0061] (4) After the enzymatic hydrolysis is completed, inactivate the enzyme in a boiling water bath for 5 minutes, take it out and cool it in cold water. Transfer the cooled sample solution to a 400ml plugged centrifuge cup and centrifuge at 4000r / min for 20min, collect the upper oil body, and store it in a 4°C refrigerator for later use;
[0062] (5) Take 5g of oil body, mix the oil body an...
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