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Populus hopeiensis genetic transformation method

A genetic transformation method and culture medium technology, applied in biochemical equipment and methods, botany equipment and methods, using vectors to introduce foreign genetic material, etc., can solve the problems of shortening the culture time, complicated culture procedures, and long culture cycles, etc. Achieve the effect of omitting the cultivation procedure and shortening the cultivation cycle

Inactive Publication Date: 2020-08-07
INNER MONGOLIA AGRICULTURAL UNIVERSITY
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Problems solved by technology

[0004] The purpose of the present invention is to provide a method for the genetic transformation of Hebei poplar in view of the above-mentioned technical deficiencies in the existing Hebei poplar genetic transformation such as complex training procedures and long training periods. By using aseptic seedling leaves as explants and Bacillus co-culture method for genetic transformation, simplifies the culture procedure, shortens the culture time, and provides a new way for the genetic transformation, quality improvement and gene function verification of Populus

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  • Populus hopeiensis genetic transformation method
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Embodiment Construction

[0020] The technical solutions of the present invention will be described in further detail below with reference to the accompanying drawings and embodiments.

[0021] The invention provides a Hebei poplar genetic transformation method, such as Figure 1 ~ Figure 4 As shown, a preferred embodiment of the present invention is shown therein. Specifically, the Hebei poplar genetic transformation method includes the following steps:

[0022] (1) Take 2-6 leaves of Populus hebei aseptic seedlings as explants, after scratching evenly several times along the main vein, inoculate the abaxial side upwards on the D medium for pre-cultivation in the dark for 1 day; wherein, the D The medium is MS+TDZ0.30 mg / L+IBA0.40 mg / L+sucrose 30g / L+agar 6g / L, and the pH value is 5.95-5.98.

[0023] (2) Take out the preserved Agrobacterium strains from the -80°C refrigerator, use a sterile pipette tip to dip into the strain preservation solution, draw a plate on the solid medium supplemented with 50...

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Abstract

The invention relates to a populus hopeiensis genetic transformation method. Aseptic seedling leaves are used as explants for genetic transformation; adventitious buds are directly induced without a callus induction stage; according to the method, a complex culture procedure is omitted, the culture period is shortened, an efficient genetic transformation system of populus hopeiensis is establishedby utilizing an agrobacterium-mediated method, a new populus hopeiensis variety is cultured, function research can be performed on related genes, and the method has important significance for furtherestablishing a quick gene function verification system.

Description

technical field [0001] The invention belongs to the technical field of transgenic engineering, and in particular relates to a genetic transformation method of Populus hebei mediated by Agrobacterium using leaves as receptors. Background technique [0002] Hebei poplar (Populus hopeiensis Hu et Chow) is a tree of the genus Populus in the family Salicaceae, with a height of 30m. The bark is yellow-green to off-white, smooth, and the crown is round. Leaves are ovate or nearly round, 3-8cm long and 2-7cm wide. The male inflorescence is about 5cm long, the rachis of the inflorescence is densely hairy, and the female inflorescence is 3-5cm long. Capsule long ovate, with short handle. The flowering period is April, and the fruiting period is May-June. It is mainly distributed in the provinces and regions of North China and Northwest China. It is one of the common poplar trees in the mountainous areas of Hebei Province. It is cultivated in various places and mostly grows on both...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/82A01H5/00
CPCC12N15/8205
Inventor 白玉娥代金玲李佳琪乌日罕
Owner INNER MONGOLIA AGRICULTURAL UNIVERSITY
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