Bio-enzyme curing material and cured silty soil roadbed combination part and construction process for reinforcing roadbed by sand compaction piles
A solidification material and bio-enzyme technology, which is applied in infrastructure engineering, roads, roads, etc., can solve the problems of difficulty in widening old and new roadbeds in silty soil, instability of roadbeds at the widening area, etc., to ensure stability and vehicle driving safety performance, ease the pressure on traffic services, and reduce the effect of liquefaction
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Embodiment 1
[0075] A biological enzyme material acting on the silty roadbed, that is, the biological enzyme in the A-type biological enzyme material consists of 20 parts of iron bacteria and 20 parts of carbonate mineralizing bacteria.
[0076] The above-described A-type biological enzyme material preparation process comprises the following steps:
[0077] Step 1) select iron bacteria as the target strain, and prepare for activation in the medium for the next step.
[0078] Step 2) Prepare 300mL iron bacteria culture solution and place it in a spherical bottle. After sterilization, use an inoculation loop to pick some strains into the culture solution. Finally, put it into a constant temperature shaking incubator with a temperature of 35° C. and a rotation speed of 180 rpm.
[0079] The nutrient solution is characterized in that the following ingredients are prepared in parts by weight:
[0080] Ferric ammonium citrate solid, 40 parts of ferric ammonium citrate liquid with a concentrati...
Embodiment 2
[0093] A biological enzyme material acting on silt roadbed, that is, type A biological enzyme material is composed of 30 parts of iron bacteria and 20 parts of carbonate mineralizing bacteria, and is used for shear strength tests of new and old silty soils, unconfined compression Stiffness test. After testing, the OD600 value of type A biological enzyme material is 1.0, and the enzyme activity value of type A biological enzyme material is 10ms / cm / min.
[0094] The biological enzyme immobilized material, that is, the sample preparation method of type A biological enzyme material and the method of applying type A biological enzyme immobilized material to sample preparation and new and old silt tests are the same as in Example 1.
Embodiment 3
[0096]A biological enzyme material acting on silt roadbed, that is, A-type biological enzyme material is composed of 40 parts of iron bacteria and 20 parts of carbonate mineralizing bacteria, which is used for shear strength tests of new and old silty soils, unconfined compression Stiffness test. After testing, the OD600 value of type A biological enzyme material is 1.2, and the enzyme activity value of type A biological enzyme material is 14ms / cm / min.
[0097] The biological enzyme immobilized material, that is, the sample preparation method of type A biological enzyme material and the method of applying type A biological enzyme immobilized material to sample preparation and new and old silt tests are the same as in Example 1.
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