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Virus removal and rapid propagation method for stem tips of dracaena sanderiana

A technique for detoxifying the tip of the stem, fortune-telling bamboo, which is applied in horticultural methods, botanical equipment and methods, horticulture and other directions to achieve the effects of accelerating the growth of clump buds, with low cost and simple operation.

Active Publication Date: 2020-08-14
湛江市兰盈农业科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the existing reports, there is no related technology related to the virus-free and rapid propagation of bamboo shoot tips

Method used

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  • Virus removal and rapid propagation method for stem tips of dracaena sanderiana
  • Virus removal and rapid propagation method for stem tips of dracaena sanderiana
  • Virus removal and rapid propagation method for stem tips of dracaena sanderiana

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Embodiment 1 Adopt the following steps to realize the present invention:

[0034] (1) Preparation of attenuated seedlings: Retrieve healthy Lucky Bamboo from the planting field, cut the stems of Lucky Bamboo into 10-15 cm stems, treat them in a 52°C incubator for 30 minutes, and place them under the conditions of a 38°C constant temperature and light incubator Cultivate for 5-6 weeks to form lateral buds; figure 1 shown;

[0035] (2) Material disinfection: Cut off the lateral buds, remove the leaves and leaf sheaths, rinse them with sterile water, then soak them in 75% alcohol for 30 s, then put them in 2% sodium hypochlorite solution for 20 minutes, and finally wash them with sterile water. Rinse with water for 3~5 times until no foam is produced;

[0036] (3) Dark treatment of materials: Cut off the part that is in contact with the disinfectant solution, cut it to a distance of 1 cm from the growth point, gently peel off the old leaves outside, cut in half longitudi...

Embodiment 2

[0043] Embodiment 2 Adopt the following steps to realize the present invention:

[0044] (1) Preparation of attenuated seedlings: Take healthy bamboo stems, cut 15-20cm, first treat them in an incubator at a temperature of 50±2°C for 35 minutes, and then cultivate them at a constant temperature for 6 weeks to form lateral buds; ±1°C, light intensity 3000lux;

[0045] (2) Material disinfection: cut off the lateral buds cultivated in step (1), remove the leaves and leaf sheaths, rinse them with sterile water, then soak them in 75% alcohol for 30 s, then put them in 2% sodium hypochlorite solution and shake for 20 min, and finally rinse with sterile water for 3 to 5 times until no foam is produced;

[0046] (3) Dark treatment of materials: Excise the part of the material washed in step (2) that has been exposed to alcohol and sodium hypochlorite solution, remove the old leaves, cut in half longitudinally, and inoculate the cross-section in the dark treatment medium MS+2, 4-D 0....

Embodiment 3

[0053] Embodiment 3 Adopt the following steps to realize the present invention:

[0054] (1) Preparation of attenuated seedlings: Take healthy bamboo stems, cut 15-20cm, first treat in an incubator at a temperature of 55°C for 30±5min, and then cultivate at a constant temperature for 5 weeks to form side buds; incubator at a temperature of 38 ±1°C, light intensity 3000lux;

[0055] (2) Material disinfection: cut off the lateral buds cultivated in step (1), remove the leaves and leaf sheaths, rinse them with sterile water, then soak them in 75% alcohol for 30 s, then put them in 2% sodium hypochlorite solution and shake for 20 min, and finally rinse with sterile water for 3 to 5 times until no foam is produced;

[0056] (3) Dark treatment of materials: Excise the part of the material washed in step (2) that has been exposed to alcohol and sodium hypochlorite solution, remove the old leaves, cut in half longitudinally, and inoculate the cross-section in the dark treatment mediu...

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Abstract

The invention discloses a virus removal and rapid propagation method for stem tips of dracaena sanderiana. The method specifically comprises the following steps: (1) preparation of attenuated seedlings; (2) disinfection of the material; (3) dark treatment of the material; (4) induction of cluster buds; (5) detection of badnavirus of virus-free seedlings; (6) subculture proliferation of the clusterbuds; (7) rooting culture; (8) seedling exercising; and (9) material transplantation. The virus removal and rapid propagation method for the stem tips of the dracaena sanderiana is simple to operateand low in cost, a large quantity of high-quality virus-free seedlings are obtained efficiently in a short time, variety degeneration caused by vegetative cutting propagation is avoided, and an effective way is provided for large-scale industrial seeding culture.

Description

technical field [0001] The invention relates to the field of rapid propagation and breeding of ornamental flowers, in particular to a method for detoxification and rapid propagation of the stem tip of lucky bamboo. Background technique [0002] Lucky Bamboo is an evergreen shrub-like plant belonging to the Agaveaceae Dracaena, also known as "Wannian Bamboo", "Transfer Bamboo", "Lucky Bamboo", "Lucky Bamboo" and so on. It is native to the African Congo. Because of its bamboo-like shape, unique shape, various forms, full of bamboo charm, noble and elegant, high ornamental value, and implying auspiciousness, peace, wealth and hard work, it is deeply loved by people. [0003] The existing lucky bamboo species have been planted for more than 20 years, with poor genetic diversity, no purification and rejuvenation, serious degradation of species, single species; Fungal diseases such as fungal diseases have made the development of the lucky bamboo industry a severe situation. Once...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/008Y02P60/40
Inventor 殷学贵陆建农黄邦盈张力江宇健
Owner 湛江市兰盈农业科技有限公司
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