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Method for studying function and mechanism of m6A reader YTHDF1 in ovarian cancer

An ovarian cancer and reader technology, applied in the field of RNA epigenetics and gynecological tumors, can solve the problems that the relationship between ovarian cancer has not been reported

Inactive Publication Date: 2020-08-14
CHONGQING UNIV CANCER HOSPITAL
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Problems solved by technology

[0006] The above studies show that m 6 A participates in the regulation of various physiological and pathological processes, but its relationship with ovarian cancer has not been reported

Method used

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  • Method for studying function and mechanism of m6A reader YTHDF1 in ovarian cancer

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Embodiment Construction

[0073] The present invention will be described in further detail below in conjunction with the accompanying drawings and specific embodiments.

[0074] A method to study the function and mechanism of m6A reader YTHDF1 in the occurrence of ovarian cancer. Firstly, it is determined that the expression of YTHDF1 is abnormal in ovarian cancer tissues; further studies on the involvement of YTHDF1 in the development of ovarian cancer in ovarian cancer cell lines and mouse models Specific functions; determine the downstream target genes regulated by YTHDF1 through MeRIP-seq combined with RIP-seq, RNA-seq, polysome-profiling and other methods, and clarify its mechanism of action; finally, the above research results are verified in clinical samples to provide clinical diagnosis and treatment direction. Technical lines such as figure 1 As shown, the method includes the following steps:

[0075] 1) Expression of YTHDF1 in ovarian cancer tissues and normal tissues

[0076] To study the...

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Abstract

The invention discloses a method for studying the function and mechanism of an m6A reader YTHDF1 in ovarian cancer. The method comprises the following steps of studying the genetic variation and expression change of an m6A modification related gene in ovarian cancer to determine that the m6A reader YTHDF1 is a main study object; carrying out further studying on the function of YTHDF1 in ovarian cancer cells and mouse models; screening and determining a target gene of the YTHDF1 by comprehensively applying a plurality of high-throughput analysis methods; and carrying out further exploration onthe clinical application feasibility of taking the YTHDF1 as the ovarian cancer molecular target. The invention provides a theoretical basis for clarifying the relationship between m6A and ovarian cancer and defining the carcinogenic function of YTHDF1, and possibly provides a new effective target for clinical diagnosis and treatment of ovarian cancer.

Description

technical field [0001] The invention relates to RNA epigenetics and gynecological tumors, in particular to a method for studying the function and mechanism of m6A reader YTHDF1 in ovarian cancer. Background technique [0002] Ovarian cancer (OC) is a type of reproductive system solid tumor derived from ovarian epithelial cells. Its clinical manifestation is occult, develops rapidly, and the prognosis is poor. It is the main cause of female death among gynecological malignancies. More than 70% of ovarian cancer patients are diagnosed at an advanced stage. According to global statistics, there are approximately 200,000 new cases of ovarian cancer and 115,000 deaths each year, with a mortality rate as high as 60%. At present, the main means of clinical treatment of ovarian cancer is surgical resection combined with platinum-based chemotherapy, but some advanced patients, especially relapsed patients, often develop resistance to chemotherapy drugs, which is also the main facto...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6886C12N15/867C12N15/90G01N33/574A61K49/00A01K67/027
CPCC12Q1/6886C12N15/86C12N15/907G01N33/57449G01N33/57484A61K49/0008A01K67/0271C12Q2600/154C12Q2600/158C12N2740/15043C12N2800/107G01N2333/47A01K2267/03A01K2207/12A01K2227/105
Inventor 邹冬玲周琦王海霞龙行涛袁犁
Owner CHONGQING UNIV CANCER HOSPITAL
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