An improved light field microscopy imaging device and construction method

Abstract

The invention discloses an improved light field microscopic imaging device and a construction method, wherein the light field microscopic imaging device includes a microscope objective lens, a microscope tube lens, a microlens array and a camera sensor, and the camera sensor collects images from the microscope objective lens on the microscope The image on the tube lens; it is characterized in that: the microlens array is located between the microscope image plane and the camera sensor, the microscope tube lens is imaged on the microscope image plane, and then imaged on the camera sensor again after the action of the microlens array . The invention obtains the distance between the microlens array and the microscope image plane according to the defocus distance definition; obtains the distance between the camera sensor and the microlens array according to the F number matching principle. The improved light field microscopic imaging device provided by the invention can obtain high reconstruction resolution near the focal plane of the objective lens, and can be used as an imaging device of a light field microscopic particle image velocimetry system.

Description

technical field [0001] The invention relates to an improved light-field microscopic imaging device, which can be applied to a light-field Micro-PIV (Light-field Micro-PIV) system and belongs to the technical field of microscale fluid measurement. Background technique [0002] The light-field microscopic imaging device can realize the recording of the three-dimensional light field information of the tracer particles in one exposure, and the light-field micro-particle image velocimetry (Light-field Micro-PIV) system based on the light-field microscopic imaging device can effectively improve the time resolution The rate is beneficial to the instantaneous measurement and characteristic research of micro-scale flow. The light field microscopy imaging device consists of a laser light source, an inverted fluorescence microscope and a cage light field camera. In an inverted fluorescence microscope, the volume light source formed by the laser is refracted by the objective lens befor...

AI Technical Summary

Problems solved by technology

[0005] The technical problem to be solved by the present invention is to provide an improved light field microscopic imaging device and its construction method, aiming at the fact that the traditional light field microscopic imaging device cannot meet the requirements of high image quality and high reconstruction resolution at the same time. The best reconstruction resolution is obtained near the plane

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