Construction method of carotid atherosclerosis plaque animal model

Abstract

The invention provides a construction method of a carotid atherosclerosis plaque animal model, and belongs to the field of animal model construction. According to the construction method, the common carotid artery intima of the central auricular artery injury is combined with high-fat feed for feeding and modeling. According to the invention, the carotid atherosclerosis plaque animal model is successfully constructed. The construction method comprises the step of modeling through a rabbit ear central artery approach; the animal model is simple in structure, small in wound, simple and convenient to operate, high in animal survival rate, high in modeling success rate and small in influence of other factors, solves the problems existing in construction of the carotid atherosclerosis plaque animal model in the prior art, can be used for screening drugs for treating atherosclerosis plaque and the like, and has important significance in research of carotid atherosclerosis.

Description

technical field [0001] The invention belongs to the field of animal model construction, and in particular relates to a method for constructing a carotid atherosclerotic plaque animal model. Background technique [0002] Atherosclerosis is a lesion in which a layer of wax-like lipid is deposited on the arterial wall, which reduces the arterial elasticity and narrows the lumen. When these waxy deposits form in one piece, it is called an atherosclerotic plaque. These plaques are flat on the intima of the blood vessel in the early stage, usually distributed longitudinally along the blood vessel, and then if they continue to progress, the plaque will slowly protrude inward on the vessel wall, causing varying degrees of stenosis of the inner diameter of the artery; if there are predisposing factors, When part of the plaque in an artery ruptures, a blood clot forms to block the blood vessel. Carotid atherosclerosis is associated with many cardiovascular and cerebrovascular diseas...

AI Technical Summary

Problems solved by technology

Among them, the method of implanting silicone rubber rings has a large surgical trauma and low animal survival rate; the electrical stimulation method has many procedures, complicated operations, time-consuming, and feasibility is questionable; the air modeling method does not have a definite gas flow rate, and the degree of damage to the intima The difference is large, and the complete occlusion of the carotid artery will inevitably lead to the histological changes of acute ischemia in the brain tissue and the blood vessel itself, artificially changing the cerebral hemodynamics

The balloon injury method is currently the most used method, but most of them are constructed in the thoracic aorta, abdominal aorta, femoral artery or carotid artery of animals, and there are also problems such as large trauma, difficult operation, and low survival rate of animals

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