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Anti-cancer pharmaceutical composition

A composition and drug technology, applied in the field of cancer treatment drugs, to achieve the effect of less toxic and side effects

Active Publication Date: 2020-08-18
INST OF ANIMAL SCI OF CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, its effects on other types of tumor cells, especially the selective inhibition of the survival and migration of liver cancer cells, have not been reported.

Method used

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  • Anti-cancer pharmaceutical composition
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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Example 1 Inhibiting the Survival Effect of Liver Cancer Cells

[0028] In order to detect the effect of phyllidine, lactoferrin, and phyllidine combined with lactoferrin on the survival of HepG2 cells, HepG2 cells were seeded in 96-well culture dishes (10 5 cells / well, 100 mL medium per well), after 24 hours of culture, 10 mg / L, 50 mg / L, 100 mg / L, 200 mg / L, 500 mg / L and 1 g / L Nantianine, 10 mg / L, 50 mg / L, 100 mg / L, 200 mg / L, 500 mg / L and 1 g / L of lactoferrin, and a combination of nantianine and lactoferrin (200 mg / L dose of nantiana combined with 100 mg / L dose of lactoferrin (mass ratio 2:1), 100 mg / L dose of nantiana combined with 100 mg / L dose of lactoferrin (mass ratio 1:1), 100 mg / L dose of nantianan combined with 200 mg / L dose of lactoferrin (mass ratio 1:2) (a total of 20 microliters for each group, 10 microliters for each drug in the combined administration group), the effect After 24 hours, discard the medium, add MTT (final concentration 5 g / L) for 4 hours, ...

Embodiment 2

[0031]Example 2 Effect of Bambooline and Lactoferrin in Inhibiting HepG2 Tumor Growth in Nude Mice

[0032] The following groups were set up: no treatment control group, 25 mg / kg dose of nantiana, 50 mg / kg dose of lactoferrin, 25 mg / kg dose of nantiana combined with 50 mg / kg lactoferrin, 12.5 mg / kg dose Nantianning bamboo base combined with 25 mg / kg lactoferrin was used to detect the growth of HepG2 tumor in nude mice.

[0033] The experimental method is as follows: HepG2 cells were cultured on a large scale in 20 10cm culture dishes, and when the cell density reached about 80%, the cells were collected and injected subcutaneously into the right armpit of 20 BALb / C nude mice. After about three weeks, the tumor volume reached 90-110mm 3 . Mice (body weight 20 ± 1 g) were divided into 5 groups of 4 mice each. With 25mg / kg dose of nantiana, 50 mg / kg dose of lactoferrin, 25 mg / kg dose of nantiana combined with 50 mg / kg lactoferrin, 12.5 mg / kg dose of nantiana combined with 25mg...

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Abstract

The invention belongs to the technical field of medicines for treating cancers, and particularly relates to an anti-cancer pharmaceutical composition. The pharmaceutical composition is prepared from nandinine and lactoferrin. The two compounds are natural compounds; the raw materials are respectively derived from plant and animal derived foods; the pharmaceutical composition containing the two components is simple in preparation method and small in toxic and side effects, can effectively inhibit the survival rate of cancers, especially liver cancer cells, in a multi-target and multi-mechanismmanner, can exert synergistic interaction activity compared with a single compound (nandinine or lactoferrin) acting group, and can be used for clinical treatment and adjuvant treatment of cancers, especially liver cancer.

Description

technical field [0001] The invention belongs to the technical field of drugs for treating cancer, and in particular relates to an anti-cancer drug composition. Background technique [0002] Liver cancer is the sixth most common cancer and the fourth leading cause of cancer-related death worldwide, posing a serious threat to human health (Dubé C, et al. Amer. J. Gastro., 2017.). Liver cancer can be divided into primary and secondary two categories. Primary liver malignant tumors originate from the epithelial or mesenchymal tissues of the liver. Primary liver cancer includes hepatocellular carcinoma, cholangiocellular carcinoma and their mixed types. Among them, hepatocellular carcinoma is the most common liver malignant tumor, accounting for primary 45% of malignant liver cancer (El-Serag, et al. Gastroenterology, 2017.; Johnson et al., J. Clinical Oncology, 2013.). Secondary or metastatic liver cancer refers to the invasion of the liver by malignant tumors originating fr...

Claims

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Application Information

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IPC IPC(8): A61K38/40A61K31/4375A61P35/00
CPCA61K31/4375A61K38/40A61P35/00A61K2300/00
Inventor 李慧颖郑楠王加启杨怀谷姚倩倩范琳琳张养东
Owner INST OF ANIMAL SCI OF CHINESE ACAD OF AGRI SCI
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