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Preparation method and application of a new phenolic acid compound with antioxidant activity

A technology of antioxidant activity and compounds, applied in the field of traditional Chinese medicine, can solve problems such as carcinogenicity and toxicity

Active Publication Date: 2020-10-30
JIANGXI UNIVERSITY OF TRADITIONAL CHINESE MEDICINE +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the potential risks of carcinogenicity and toxicity of synthetic antioxidants, it is an inevitable trend in the development of antioxidants in today's food science to find safe, effective, and non-toxic natural antioxidants.

Method used

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  • Preparation method and application of a new phenolic acid compound with antioxidant activity
  • Preparation method and application of a new phenolic acid compound with antioxidant activity
  • Preparation method and application of a new phenolic acid compound with antioxidant activity

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] The method for preparing this phenolic acid compound comprises the steps:

[0026] (1) Heat and reflux the stems and leaves of Xiaohuaqingfengteng with 12 times the amount of 70% ethanol solution to extract 3 times, each time for 2 hours, filter, and concentrate the filtrate under reduced pressure to obtain a crude extract;

[0027] (2) Dissolve the crude extract obtained in step (1) with 30% ethanol, and filter to obtain the supernatant;

[0028] (3) Take the supernatant obtained in step (2) and load it on HP-20 macroporous resin column (sample:resin is filled according to the volume ratio of 1:3), followed by water, 30% ethanol, 50% ethanol, 75 % ethanol, 95% ethanol, ethanol elution, 3 column volumes for each gradient elution. Take the eluted part of 30% ethanol and pass it through ODS medium and low pressure reversed phase liquid chromatography column (50*500mm), MeOH-H 2 O (20%, 30%, 40%, 50%, 60%, 70%, 80%) was eluted to obtain 7 fractions A-G. D was subjected ...

Embodiment 2

[0030] The method for preparing the compound of formula 1 structure, comprises the steps:

[0031] (1) Heat and reflux the stems and leaves of Xiaohuaqingfengteng with 20 times the amount of 95% ethanol solution to extract twice, each time for 3 hours, filter, and concentrate the filtrate under reduced pressure to obtain a crude extract;

[0032] (2) Dissolve the crude extract obtained in step (1) with 30% ethanol, and filter to obtain the supernatant;

[0033] (3) Take the supernatant obtained in step (2) and load it on HP-20 macroporous resin column (sample:resin is filled according to the volume ratio of 1:3), followed by water, 30% ethanol, 50% ethanol, 75 % ethanol, 95% ethanol, ethanol elution, 3 column volumes for each gradient elution. Take the eluted part of 30% ethanol and pass it through ODS medium and low pressure reversed phase liquid chromatography column (50*500mm), MeOH-H 2 O (20%, 30%, 40%, 50%, 60%, 70%, 80%) was eluted to obtain 7 fractions A-G. D was sub...

Embodiment 3

[0035] The method for preparing the compound of formula 1 structure, comprises the steps:

[0036] (1) Extract the stems and leaves of Xiaohuaqingfengteng with 30 times the amount of 75% ethanol solution cold soaking method for 3 times, each time for 24 hours, filter, and concentrate the filtrate under reduced pressure to obtain a crude extract;

[0037] (2) Dissolve the crude extract obtained in step (1) with 30% ethanol, and filter to obtain the supernatant;

[0038] (3) Take the supernatant obtained in step (2) and load it on HP-20 macroporous resin column (sample:resin is filled according to the volume ratio of 1:3), followed by water, 30% ethanol, 50% ethanol, 75 % ethanol, 95% ethanol, ethanol elution, 3 column volumes for each gradient elution. Take the eluted part of 30% ethanol and pass it through ODS medium and low pressure reversed phase liquid chromatography column (50*500mm), MeOH-H 2 O (20%, 30%, 40%, 50%, 60%, 70%, 80%) was eluted to obtain 7 fractions A-G. D...

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Abstract

The invention relates to an application of a novel phenolic acid compound in preparation of an antioxidant drug, and also relates to a preparation method for extracting, separating and purifying the compound from a Chinese herbal medicine, namely sabia parviflora. The preparation method is simple and feasible, and the obtained compound is high in purity. Pharmacological studies show that the novelcompound has a good capability of scavenging free radicals in vitro, and can reduce the generation of reactive oxygen species (ROS) in H2O2-induced macrophages RAW264.7. In addition, the activity ofsuperoxide dismutase (SOD) in cells can be improved. It is indicated that the compound has obvious antioxidant activity and can be used for preparing the antioxidant drug. The compound has good economic benefits and application prospects.

Description

technical field [0001] The invention belongs to the technical field of traditional Chinese medicine, and specifically relates to a preparation method for extracting and separating phenolic acid compounds from the stems and leaves of Qingfeng vine, a traditional Chinese medicine extract or mixture containing the compounds, and an application in preparing antioxidant drugs. Background technique [0002] Oxidative stress has a very important impact on human aging and health. Excessive free radicals caused by various factors can trigger and aggravate many diseases, such as Alzheimer's disease, cardiovascular disease, malignant tumors, etc. Therefore, the application of antioxidants To maintain human health has a wide range of needs. Due to the potential risks of carcinogenicity and toxicity of synthetic antioxidants, it is an inevitable trend in the development of antioxidants in today's food science to find safe, effective, and non-toxic natural antioxidants. h 2 o 2 It is a...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07C65/40C07C51/42C07C51/47A61K31/194A61P39/06
CPCA61P39/06C07C51/42C07C51/47C07C65/40
Inventor 李志峰冯育林王琦杨世林杜伟东黄文平游梦
Owner JIANGXI UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
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