Antioxidant with function of inhibiting melanin production as well as preparation method and use of antioxidant

A technology for inhibiting melanin and anti-oxidants, applied in skin care preparations, functions and applications of food ingredients, etc., to achieve broad application prospects, maintain activity, and simple steps

Inactive Publication Date: 2018-07-24
ANHUI UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no relevant report on the isolation of a homogeneous polysaccharide component that has both antioxidant and melanin production inhibitory effects from the fruiting body of Morchella.

Method used

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  • Antioxidant with function of inhibiting melanin production as well as preparation method and use of antioxidant
  • Antioxidant with function of inhibiting melanin production as well as preparation method and use of antioxidant
  • Antioxidant with function of inhibiting melanin production as well as preparation method and use of antioxidant

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Experimental program
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Effect test

Embodiment 1

[0033]In the present embodiment, the preparation method of an antioxidant capable of inhibiting melanin production is as follows:

[0034] 1. Pulverize the Morchella fruiting bodies and pass through an 80-mesh sieve to obtain powder;

[0035] 2. Add 100 g of the obtained powder in sequence to petroleum ether and 95vt% ethanol and reflux at 60°C for 2 hours, repeat three times, and dry the obtained precipitate for later use;

[0036] 3. Take 100 g of the precipitate obtained in step 2, add it into distilled water at a liquid-to-solid ratio of 22 mL / g, and conduct ultrasonic-assisted extraction. The ultrasonic power is 300 W, and the ultrasonic time is 30 min. Repeat the extraction 3-4 times, and combine the extracts;

[0037] 4. Concentrate the extract obtained in step 3 to 200mL, add four times the volume of 95vt% ethanol, let stand at 4°C for 10h, centrifuge, and collect the precipitate;

[0038] 5. Dissolve the precipitate obtained in step 4 with distilled water, then add a...

Embodiment 2

[0041] Embodiment 2: In vitro free radical scavenging ability test

[0042] 1. Hydroxyl radical scavenging experiment

[0043] Accurately prepare morel polysaccharide FMP solution (25, 50, 100, 200, 300 and 400μg / mL), 6mM ferrous sulfate solution, 2.4mM hydrogen peroxide solution, 6mM ethanol-salicylic acid solution, each take 1mL sulfuric acid Ferrous solution, 1mL ethanol-salicylic acid solution, add 1mL polysaccharide sample solution, oscillate and mix well and let stand for 10min. Add 1 mL of hydrogen peroxide solution and react for 30 min. Ascorbic acid was used as a positive control, and the reaction was carried out in a constant temperature water bath at 37°C for 30 minutes and protected from light. Absorbance at 510nm with a UV-Vis spectrophotometer. Deionized water was used instead of polysaccharide solution as a blank group, and instead of salicylic acid as a control group. Three parallel groups were set up for each experiment. Calculation of hydroxyl radical sca...

Embodiment 3

[0048] Embodiment 3: In vitro inhibition of melanin activity test

[0049] 1. Determination of FMP polysaccharides on the proliferation of B16F10 cells

[0050] MTT method was used to measure the effect of samples on the proliferation of B16F10 cells, and the concentration of passaged cells was adjusted to 5×10 3 After each well, add 96-well culture plate, 100 μL per well; culture for 24 hours, absorb the culture medium after the cells adhere to the wall, replace the medium once with fresh culture medium treated with different drug concentrations, set up 8 replicate wells in each group, and place At 37°C, 5% CO 2 Cultivate under conditions for 48 hours; add MTT solution (5 mg / mL, 20 μL / well) 4 hours before the end; discard the supernatant after 48 hours, add 100 μL dimethyl sulfoxide to each well, shake gently for 5 minutes to fully dissolve the crystals; then The 96-well plate was placed in a microplate reader, and the absorbance was measured at a wavelength of 570 nm. The...

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Abstract

The invention discloses an antioxidant with a function of inhibiting melanin production as well as a preparation method and use of the antioxidant. The antioxidant is a polysaccharide component FMP separated out from morchella esculenta, and has a molecular weight of 4.5kDa; the monosaccharide composition mainly is prepared from mannose, glucose and galactose. The polysaccharide component FMP obtained by the method has good in-vitro free radical scavenging ability; in vitro and in vivo experiments prove that the polysaccharide component FMP can inhibit the melanin production and tyrosinase activity well, and does not have obvious toxic and side effects; the tyrosinase inhibition activity of the polysaccharide component FMP is comparable to that of kojic acid; therefore, the polysaccharidecomponent FMP can be used as a natural high-efficiency antioxidant and a melanin production inhibitor, and has a great application prospect in the fields such as food and cosmetics.

Description

technical field [0001] The invention relates to an antioxidant capable of inhibiting melanin production, a preparation method and application thereof, and belongs to the field of food processing. Background technique [0002] Melanin is the end product of L-tyrosine undergoing a complex transformation, mainly present in human skin, hair and eyes, and plays an important role in protecting against the effects of solar ultraviolet radiation. However, excessive or abnormal synthesis of melanin can lead to skin pigmentation, causing symptoms such as freckles, chloasma, and actinic keratosis. Among the current whitening agents, kojic acid and arbutin are the most commonly used, but they have their own shortcomings. It has been reported that kojic acid may cause cancer and is potentially dangerous. The effect of arbutin on melanin content is bidirectional, that is, it can inhibit the synthesis of melanin at a certain concentration, and increase the content of melanin at another c...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K8/73A61Q19/02A61Q19/00A23L31/00A23L33/00
CPCA23L31/00A23L33/00A23V2002/00A61K8/73A61K2800/522A61K2800/782A61Q19/00A61Q19/02A23V2200/318A23V2250/208A23V2300/14
Inventor 陆永明蔡正楠李婉王亚陈彦
Owner ANHUI UNIVERSITY
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