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Application of crocin ii in the preparation of medicine for preventing and treating hair loss by acting on hair follicle tissue

A technology of crocin and hair follicles, applied in the field of medicine, can solve the problems such as the prevention and treatment of hair loss, etc., which are not seen with crocin II monomer, and achieve the effect of promoting cell proliferation.

Inactive Publication Date: 2021-06-01
上海沸米生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no report on the prevention and treatment of hair loss and the improvement of hair follicle tissue survival by crocin II monomer

Method used

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  • Application of crocin ii in the preparation of medicine for preventing and treating hair loss by acting on hair follicle tissue
  • Application of crocin ii in the preparation of medicine for preventing and treating hair loss by acting on hair follicle tissue
  • Application of crocin ii in the preparation of medicine for preventing and treating hair loss by acting on hair follicle tissue

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Embodiment 1: Effect of crocin II on growth of mouse hair follicles and proliferation of hair follicle bulb cells in vitro

[0033] 1. Preparation of saffron decoction

[0034] Take 40g of saffron (purchased from Beijing Tongrentang Pharmaceutical Co., Ltd.), soak in 400mL of distilled water for 1h, decoct in a ceramic decocting pot for 40min, pour out the medicinal liquid, add 200mL of distilled water to the medicinal residue and decoct for 20min, and combine the two decoctions , concentrated to 200mL over low heat, so that the concentration of the saffron peanut medicine in the liquid medicine was 0.20g / mL, adjusted to a pH value of about 7.4 after suction filtration, sterilized by high-pressure steam, and stored at 4°C for later use.

[0035] 2. Main experimental materials

[0036] C57BL / 6J mice (provided by the Institute of Animals, Chinese Academy of Medical Sciences), crocin II with a purity of 99% (Sigma Company), Williams culture medium, isolate enzyme and type I...

Embodiment 2

[0062] Example 2: Effect of crocin II on the Wnt / β-catenin signaling pathway of human scalp hair follicle tissue in vitro culture

[0063] 1. Main reagents and medicines

[0064] Crocin II purity 99% (Sigma Company); rabbit anti-human Wnt3a monoclonal antibody, rabbit anti-human β-catenin monoclonal antibody, β-actin (Sigma Company), RPM11640 medium (Sigma Company).

[0065] 2. Main experimental steps

[0066] (1) Take the discarded human scalp hair follicles after hair transplantation (the patient has signed the informed consent), put the isolated specimens into a sterile centrifuge tube filled with normal saline immediately after obtaining the hair follicles, and separate the hair follicles under a microscope. The posterior hair follicle tissue was moved to a new PBS culture dish and washed three times with fresh PBS. Carefully aspirate the PBS wash solution under the microscope with a 2 mL pipette.

[0067] (2) Crocin II is dissolved with serum-free RPM11640 culture flui...

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Abstract

The invention provides the use of crocin II in the preparation of a medicine for preventing and treating hair loss by acting on hair follicle tissue. The present invention also provides the use of crocin II in the preparation of a drug for improving the activity of hair follicle tissue. The drug is directly smeared or sprayed on the scalp to promote hair follicles in the telogen phase to enter a regeneration cycle and promote hair growth. The present invention also provides the use of crocin II in the preparation of a drug for improving the activity of hair follicle tissue cultured in vitro, making the crocin II into a tissue culture solution and acting on the hair follicle tissue in vitro to improve the microenvironment of the hair follicle tissue, Promote the transformation of hair follicle tissue in the telogen and catagen states into hair follicle tissue in the anagen phase, thereby increasing the activity of the cultured hair follicle tissue, and significantly improving the survival rate of transplanted hair follicles.

Description

technical field [0001] The invention belongs to the field of medicine and relates to a new application of crocin II, in particular to the application of crocin II in preparing a medicine for preventing and treating hair loss by acting on hair follicle tissue. Background technique [0002] Hair and hair are keratinized filamentous structures that grow on the skin surface of the whole body, and are appendages derived from the epidermis. Hair and hair are unique to mammals. Human hair has been quite degenerated, but hair follicles are rich in nerve endings and are sensitive touch receptors. At the same time, human hair affects people's appearance and has important social significance. [0003] Studies have shown that hair follicles are formed during embryonic development and undergo repeated cycles of growth (Anagen), regression (Catagen) and rest (Telogen) after birth. In the growth phase, the hair mother cells (Matrix) rapidly proliferate and differentiate into the hair sha...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/7024A61K45/06A61P17/14
CPCA61K31/7024A61K45/06A61P17/14
Inventor 王浩刘树山秦春晖
Owner 上海沸米生物科技有限公司