Newcastle disease attenuated sugar vitrified vaccine and preparation method thereof

A chicken Newcastle disease, vitrification technology, applied in the direction of biochemical equipment and methods, viruses, antiviral agents, etc., can solve problems such as loss and affecting the growth of livestock, and achieve the effect of ensuring performance

Pending Publication Date: 2020-08-28
JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] In the prior art, the method adopted for this virus is inoculation prevention. As the drug resistance of the virus increases, the drug properties of the adopted vaccine gradually decrease after inoculation, causing the virus to still affect the growth of livestock, and it is easy to cause losses in severe cases.

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  • Newcastle disease attenuated sugar vitrified vaccine and preparation method thereof

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Embodiment Construction

[0028] In order to make the above objects, features and advantages of the present invention more comprehensible, specific implementations of the present invention will be described in detail below in conjunction with the accompanying drawings.

[0029] In the following description, a lot of specific details are set forth in order to fully understand the present invention, but the present invention can also be implemented in other ways different from those described here, and those skilled in the art can do it without departing from the meaning of the present invention. Similarly generalized, the present invention is therefore not limited by the specific embodiments disclosed below.

[0030] Secondly, the present invention is described in detail in conjunction with schematic diagrams. When describing the implementation of the present invention in detail, for the convenience of explanation, the cross-sectional view showing the device structure will not be partially enlarged accor...

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Abstract

The invention belongs to the technical field of veterinary drugs, and particularly relates to a Newcastle disease attenuated sugar vitrified vaccine and a preparation method thereof. The preparation raw materials of the vaccine include hatching eggs, reagents and seed virus. The reagents comprise any one or more of PBS (phosphate buffer solution) or normal saline and any one or more of a penicillin solution or a streptomycin solution; and the seed virus comprises any one or more of an HB1 strain, an F strain, a LaSota strain, a LaSota-Clone30 strain or an N79 strain, and propiolactone. The vaccine also comprises a raw material reactant for preparing the vitrified vaccine, and the reactant is specifically one or more of trehalose or agarose. According to the present invention, an erythrocyte experiment is directly performed on the generated vaccine, and the optimal attenuated virus can be found according to the optimal erythrocyte agglutination point so as to effectively ensure the performance of the vaccine.

Description

technical field [0001] The invention relates to the technical field of veterinary drugs, in particular to a Newcastle disease attenuated sugar vitrified vaccine and a preparation method thereof. Background technique [0002] Newcastle disease is an acute and highly contagious poultry infectious disease caused by Newcastle disease virus (The morbidity and mortality are increasing year by year, and there is no specific drug treatment, which has caused serious economic losses to the poultry industry. The World Organization for Animal Health (OIE) lists it as a Class A infectious disease, and my country lists Newcastle disease as a major animal disease and a Class I infectious disease. [0003] The method adopted for this virus in the prior art is inoculation prevention. As the drug resistance of the virus increases, the drug properties of the adopted vaccine gradually decrease after inoculation, causing the virus to still affect the growth of livestock, and in severe cases, it i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/12A61K9/19A61P31/14
CPCA61K9/19A61K39/12A61K2039/5254A61P31/14C12N2760/18134
Inventor 王琳琳
Owner JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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