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A kind of nano drug system targeting tumor and preparation method thereof

A nano-drug and tumor technology, applied in the field of tumor-targeting nano-drug system and its preparation, can solve the problems of affecting the overall stability of the drug-carrying system, instability of black scale nano-materials, and difficulty in storage, and achieve good tumor targeting. Sex, prolong blood circulation time, improve the effect of stability

Active Publication Date: 2021-01-08
高漓
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the instability of the black scale nanomaterial itself, it affects the overall stability of its drug-carrying system, it is difficult to preserve, and its practical use is greatly limited.

Method used

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  • A kind of nano drug system targeting tumor and preparation method thereof
  • A kind of nano drug system targeting tumor and preparation method thereof
  • A kind of nano drug system targeting tumor and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] The nano drug system is characterized by structural formula: Zn-BP-P-Apt / D NSs, and its preparation method comprises the following steps:

[0043] A. Disperse 3mg of black phosphorus crystal powder in 16ml of 1-methyl-2-pyrrolidone, and then use the probe to sonicate the mixture for 6 hours under ice bath conditions, ultrasonic power 600W, on / off cycle, The on / off cycle time is: 3 s / 3 s, the obtained brown suspension is centrifuged at 2000 rpm for 10 minutes, the supernatant is collected, and centrifuged at 10000 rpm for more than 10 minutes to collect the precipitate , to prepare BP NSs for use.

[0044] B. Dissolving 700ug of Apt-SH with an optical density of 20OD in 0.8ml tris buffer; wherein the tris content in the tris buffer is 10 mM / ml, pH = 7.4; then add 1.6mg of NH 2- PEG-MAL and 20ug of TCEP, stirred for 2 hours in the dark to obtain NH 2 - PEG-Apt solution;

[0045] C. Add 1.6mg of BP NSs to the NH obtained in the previous step 2 -PEG-Apt solution, s...

Embodiment 2

[0049] The nano drug system is characterized by structural formula: Zn-BP-P-Apt / D NSs, and its preparation method comprises the following steps:

[0050] A. Disperse 8mg of black phosphorus crystal powder in 24ml of 1-methyl-2-pyrrolidone, then put the mixture under ice bath conditions, use the probe to sonicate for 12 hours, ultrasonic power 800 W, on / off cycle , the on / off cycle time is: 4s / 6 s, the obtained brown suspension is centrifuged at 4000 rpm for 20 minutes, the supernatant is collected, and centrifuged at 10000 rpm or more for more than 10 minutes, the precipitate is collected, BP NSs were prepared and used for later use.

[0051] B. Dissolving 1300ug of Apt-SH with an optical density of 20OD in 1.4ml tris buffer; wherein the tris content in the tris buffer is 10 mM / ml, pH = 7.4; then add 2.5 mg of NH 2 - PEG-MAL and 60ug of TCEP, stirred for 5 hours in the dark to obtain NH 2 - PEG-Apt solution;

[0052] C. Add 2.4mg of BP NSs to the NH obtained in the prev...

Embodiment 3

[0056] The nano drug system is characterized by structural formula: Zn-BP-P-Apt / D NSs, and its preparation method comprises the following steps:

[0057] A. Disperse 5mg of black phosphorus crystal powder in 20ml of 1-methyl-2-pyrrolidone, and then put the mixture in an ice bath, use a probe to sonicate for 8 hours, ultrasonic power 700 W, on / off cycle , the on / off cycle time is: 2 s / 4 s, the obtained brown suspension is centrifuged at 2000-4000 rpm for 10-20 minutes, the supernatant is collected, and centrifuged again at 10000 rpm for 10 minutes Above, the precipitate was collected to prepare BP NSs for future use.

[0058] B. Dissolving 1000ug of Apt-SH with an optical density of 20OD in 1ml tris buffer; wherein the tris content in the tris buffer is 10 mM / ml, pH = 7.4; then add 2mg parts by weight of NH 2 - PEG-MAL and 40ug of TCEP, stirred for 2-5 hours in the dark to obtain NH 2 - PEG-Apt solution;

[0059] C. Add 160-240 parts by weight of BP NSs to the NH obtaine...

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PUM

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Abstract

The invention discloses a tumor targeting nano-drug system, a carrier of the tumor-targeting nano-drug system is Zn-BP-P-Apt NSs, and a characterization structural formula of the tumor-targeting nano-drug system is Zn-BP-P-Apt / D NSs. The nano-drug system disclosed by the invention is combined with chemical photothermal therapy to treat prostatic cancer, has high targeting property on cancer cells,can enhance the tumor treatment effect, and can reduce the toxic and side effects caused by chemotherapeutic drugs.

Description

technical field [0001] The invention belongs to the technical field of medicine, in particular to a tumor-targeting nano drug system and a preparation method thereof. Background technique [0002] One of the biggest killers that threaten human life and health is tumor. Chemical drugs are an indispensable means of treating prostate cancer, but chemical drugs are non-specific to cancerous cells and normal human cells, so that they not only kill cancer Kill normal cells, causing serious toxic side effects throughout the body. In recent years, searching for safe and effective targeted drugs has become a hotspot in the treatment of tumors. [0003] Nano-targeted drugs use nanoparticles as drug carriers, mainly by changing the distribution and pharmacokinetic properties of drugs in the body to ensure the targeting of drugs to tumors and other lesions. The nano drug delivery system can realize the control of drug delivery and release at the molecular level, and the passive or act...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K41/00A61K31/704A61K33/30A61K47/60A61K47/52A61K47/54A61P35/00
CPCA61K31/704A61K33/30A61K41/0052A61K41/0057A61K47/52A61K47/549A61K47/60A61P35/00A61K2300/00
Inventor 高漓
Owner 高漓
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