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In-vitro amplification method of northern pig-tailed macaque T cells

An in vitro amplification and cell technology, applied in the biological field, can solve the problems of low amplification efficiency of kits, and achieve low production costs, improved amplification efficiency, and good stability

Inactive Publication Date: 2020-09-01
HENAN UNIVERSITY +1
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For northern flat top monkey T lymphocytes, the expansion efficiency of the kit is low

Method used

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  • In-vitro amplification method of northern pig-tailed macaque T cells
  • In-vitro amplification method of northern pig-tailed macaque T cells
  • In-vitro amplification method of northern pig-tailed macaque T cells

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Embodiment Construction

[0035] Various exemplary embodiments of the present invention will now be described in detail. The detailed description should not be considered as a limitation of the present invention, but rather as a more detailed description of certain aspects, features and embodiments of the present invention.

[0036]It should be understood that the terminology described in the present invention is only used to describe specific embodiments, and is not used to limit the present invention. In addition, regarding the numerical ranges in the present invention, it should be understood that each intermediate value between the upper limit and the lower limit of the range is also specifically disclosed. Each smaller range between any stated value or intervening value in a stated range and any other stated value or intervening value in a stated range is encompassed within the invention. The upper and lower limits of these smaller ranges may independently be included or excluded from the range. ...

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Abstract

The invention discloses an in-vitro amplification method of northern pig-tailed macaque T cells, and belongs to the field of biotechnology. A commercialized non-human primate T cell activation / amplification kit is used for in vitro amplification of northern pig-tailed macaque T cells; the kit comprises magnetic beads and a biotinylated antibody, can simulate the effect of in-vivo antigen presenting cells, provides a first signal and a second signal for T cell activation, and effectively activates and purifies resting T cells in peripheral blood mononuclear cells. CD3 molecules are important markers on the surfaces of T cells and are combined with TCR through non-covalent bonds to form a TCR-CD3 complex, activation signals are transmitted into the cells, and activation and proliferation ofT cells are induced. CD28-mediated co-stimulatory signals can induce differentiation and proliferation of T cells and secretion of cytokines such as IL-2 and the like.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to an in vitro expansion method of T cells of the northern flat top monkey. Background technique [0002] Acquired immunodeficiency syndrome (AIDS) is an infectious disease caused by human immunodeficiency virus type I (HIV-1). HIV-1 virus mainly damages the body's immune system and seriously threatens human life and health. For a long time, in order to conquer HIV-1 virus infection, researchers have made many efforts. The non-human primates (NHP) model is an ideal animal model for studying the pathogenesis, vaccines and drugs of AIDS. In previous studies, primate surrogate models infected with simian immunodeficiency virus (SIV) or simian / human immunodeficiency virus (SHIV) were the most widely used HIV / AIDS animal models. However, the gene homology between SIV and SHIV virus and HIV-1 virus is quite different, which cannot truly reflect the clinical immune response of the body infe...

Claims

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Application Information

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IPC IPC(8): C12N5/0783
CPCC12N5/0636
Inventor 况轶群郑永唐汤贝贝洪琛庞伟田仁荣
Owner HENAN UNIVERSITY
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