Application of crude extract of radix tetrastigme in aspect of inhibiting pancreatic cancer
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A technology of crude extract and clover leaf, applied in biochemical equipment and methods, medical preparations containing active ingredients, drug combinations, etc., can solve the problems of low response rate, adverse reactions of chemotherapy drugs, etc., and achieve good separation Effect
Inactive Publication Date: 2020-09-04
吴善聪
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With the development and application of chemotherapy drugs such as gemcitabine, the adjuvant chemotherapy regimen of gemcitabine combined with capecitabine is the standard treatment after pancreatic cancer resection in Europe and the United States, but the adverse reactions and low response rates of chemotherapy drugs and their treatment in Asian populations The effect is still under further study, new evidence-based medical evidence is needed
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Embodiment 1
[0029] A method for obtaining the crude extract of Clover, specifically comprising the following steps:
[0030] S1. Take 155 g of the medicinal material powder of A. clover root, add 70% ethanol to carry out reflux extraction for 3 times, the reflux temperature is 80 degrees Celsius, and extract for 1.5 hours each time;
[0031] S2. Filter the solution after each extraction in step S1 with a 5 μm filter membrane to separate the extract and filter residue, combine the extracts, concentrate and dry the filtrate at 50° C., and obtain a solid crude extract. The weight of the solid crude extract is 14.17 g ;
[0032] S3, column chromatography points
[0033] S3-1. Macroporous adsorption resin: Take 600ml of HP-20 resin and soak it overnight with 95% ethanol; after suspension, install it into a glass chromatography column; first elute with 1.8L of 95% ethanol, and then equilibrate with water;
[0034] S3-2, take 13.5g of the solid crude extract of step S2, dissolve it with about ...
Embodiment 2
[0053] The death of pancreatic ductal adenocarcinoma (PDAC) cells was induced by the crude extract of Cloverleaf obtained in Example 1, which specifically included the following steps: (1) four kinds of PDAC cells were planted in 96-well plates, 5 × 10 cells per well. 3 cells; (2) after 24 hours, the cells adhered to the wall, and four components of the crude extract of Clover were added respectively: component-1 (10% (1-2) EtOH extract), component-2 (10 %(-3)-30%(1-2) EtOH extract), fraction-3 (30%(-1)-50%(-1)EtOH extract), fraction-4 (50%(- 2) -95% (-2) EtOH extract), the concentration gradient is set to be 0, 100, 250mg / L; (3) After 48 hours, add 10ul of PI dye of 0.05mg / ml to each well, and the medium in each well is 200ul system, the cell survival rate after treatment at a specified concentration was measured with a cell counting kit-8 (CCK-8).
[0054] After enrichment and separation, it was divided into four fractions: fraction-1 (10% (1-2) EtOH extract), fraction-2 (1...
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Abstract
The invention discloses application of crude extract of radix tetrastigme to preparation of a chemotherapeutic medicament for inhibiting the pancreatic cancer. The invention further discloses a medicament for inhibiting the pancreatic cancer. The medicament comprises one or more of four components as follows: a component-1 (10% (1-2) EtOH extract), a component-2 (10% (-3) to 30%(1-2) EtOH extract), a component-3(30%(-1) to 50%(-1) EtOH extract) and a component-4 (50%(-2) to 95%(-2) EtOH extract). The invention further discloses application of the crude extract of the radix tetrastigme in the aspect of inhibiting the pancreatic cancer. The application comprises the following process of: respectively implanting four types of pancreatic ductal adenocarcinoma (PDAC) cells into a 96-hole plate;carrying out cell adherence, and respectively adding four components of the crude extract of the radix tetrastigme; carrying out shooting under a fluorescence microscope; and measuring a cell survival rate by CCK-8. The invention additionally further provides a method for acquiring the crude extract of the radix tetrastigme. Application of the radix tetrastigme, which is disclosed by the invention, provides a new treatment target for the chemotherapeutic medicament and a treatment medicament for the pancreatic cancer.
Description
technical field [0001] The invention belongs to the field of medicine preparation, and in particular relates to the application of a crude extract of Clover in the preparation of chemotherapy drugs for inhibiting pancreatic cancer, a drug for inhibiting pancreatic cancer, and the use of a crude extract of Clover in the preparation of chemotherapy drugs for inhibiting pancreatic cancer. The application in the aspect of inhibiting pancreatic cancer and a method for obtaining the crude extract of A. trifoliate. Background technique [0002] Pancreatic cancer is one of the most malignant solid tumors. In recent years, the incidence of pancreatic cancer has been increasing year by year all over the world. 95% of pancreatic cancer is pancreatic ductal cell carcinoma (pancreatic ductaladenocarcinoma, PDAC). PDAC has the characteristics of high invasion and metastasis, and lacks obvious clinical symptoms and signs in the early stage. About 60% of the patients have developed distan...
Claims
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