Integrated self-service nucleic acid detection device and application method thereof

Abstract

The invention relates to an integrated self-service nucleic acid detection device and an application method thereof. The device comprises a cassette, a reaction chip and an elastic membrane, wherein piston chambers, a sample receiving chamber, a reagent chamber and a recovery chamber are formed on the cassette; two reaction cells are formed on the upper surface of the reaction chip; the elastic membrane is used for bonding the reaction chip at the bottom of the cassette; the sample receiving chamber and the reagent chamber communicate with one reaction cell through a first one-way valve structure; and the reagent chamber and the recovery chamber communicate with the other reaction cell through a second one-way valve structure. According to the integrated self-service nucleic acid detectiondevice, all processes of nucleic acid detection can be completed only through simple four-step push and pull actions of push rods, professional training and special laboratory conditions are not needed, devices, such as a pipette and a temperature cycling instrument are not needed, the operation steps of nucleic acid detection are greatly simplified, the requirements for operating personnel and operation places are reduced, the integrated self-service nucleic acid detection device can be popularized and applied in families and community clinics, and real-time screening detection during epidemic prevention and control and daily personal health monitoring are achieved.

Description

technical field [0001] The present invention relates to a nucleic acid detection device and its use method, in particular to an integrated self-service nucleic acid detection device and its use method that can simply and conveniently realize nucleic acid amplification detection at home or in any other place. Background technique [0002] Nucleic acid detection is the most direct, reliable and sensitive method in pathogen detection, and has important applications in disease diagnosis, epidemic prevention and control, health monitoring and other fields. Nucleic acid detection detects the genetic material (DNA or RNA) of the pathogen, and can determine the type, concentration and other information of the pathogen within 1 hour, avoiding the interference of factors such as the window period of disease infection. [0003] The nucleic acid detection process generally includes the following steps: sample pretreatment, nucleic acid extraction, nucleic acid amplification, and detecti...

AI Technical Summary

Problems solved by technology

Therefore, the high threshold of traditional nucleic acid detection

Images