Microsatellite molecular marker for distinguishing genetic backgrounds of chromosomes 2 of sugarcane noble species and saccharum spontaneum and application of microsatellite molecular marker

Abstract

The present invention discloses a microsatellite molecular marker for distinguishing genetic backgrounds of chromosomes 2 of sugarcane noble species and saccharum spontaneum and an application of themicrosatellite molecular marker. The microsatellite molecular marker comprises four pairs of SSR molecular marker core primer pairs located on the chromosome 2 of the sugarcane noble species. Whole genome data based on the sugarcane noble species and saccharum spontaneum are studied, SSR marker primers are designed and synthesized, and polyacrylamide gel electrophoresis verification is performed on 5 noble species materials, 4 saccharum spontaneum materials, and 2 cultivated species materials. The selected 4 pairs of the primers have characteristics of stable amplification results and clear and identifiable electrophoretic bands, chromosomal positions of the sugarcane noble species and saccharum spontaneum where SSR is located can be distinguished, confirming that the SSR marker has relatively high repetitiveness and polymorphism and can be used for sugarcane genetic analysis and variety identification.

Description

technical field [0001] The invention belongs to the technical field of sugarcane molecular breeding, in particular to a microsatellite molecular marker for distinguishing the genetic background of No. 2 chromosome of sugarcane noble species and cut hands dense species and its application. Background technique [0002] sugar cane( Saccharum hybrids ) is an important sugar crop in the world and one of the main economic crops in southern my country. Sugarcane sugar production accounts for about 80% of the world's total sugar production. At the same time, among the by-products of sugarcane sugar extraction, bagasse contains about 50% of the fiber It can be used to make paper, and the cellulose in bagasse can be converted into sugar to make alcohol. 40% of the world's alcohol is produced from sugarcane. Saccharum ( Saccharum ) including tropical species ( S. officinarum ), Chinese species ( S. sinense ), Indian species ( S. barberi ), large stem wild species ( S. robust...

AI Technical Summary

Problems solved by technology

[0005] In recent years, the application of SSR molecular markers in sugarcane has been gradually expanded. However, the publicly available sugarcane SSR molecular markers are limited in number and low in polymorphism, which cannot meet the requirements of molecular marker-assisted breeding and genetic mapping in sugarcane.

The traditional SSR marker development method has many disadvantages, such as consuming manpower, material resources, and low efficiency, especially for polyploid sugarcane, the development is more difficult

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