Microsatellite molecular marker for distinguishing genetic backgrounds of chromosomes 2 of sugarcane noble species and saccharum spontaneum and application of microsatellite molecular marker

A No. 2 chromosome and molecular marker technology, which is applied in the field of microsatellite molecular markers and applications, can solve the problems of sugarcane molecular marker-assisted breeding and genetic mapping, difficulty in development, and low polymorphism.

Active Publication Date: 2020-09-15
FUJIAN AGRI & FORESTRY UNIV
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AI Technical Summary

Problems solved by technology

[0005] In recent years, the application of SSR molecular markers in sugarcane has been gradually expanded. However, the publicly available sugarcane SSR molecular markers are limited in number and low in polymorphism, which cannot meet the requirements of molecu...

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  • Microsatellite molecular marker for distinguishing genetic backgrounds of chromosomes 2 of sugarcane noble species and saccharum spontaneum and application of microsatellite molecular marker
  • Microsatellite molecular marker for distinguishing genetic backgrounds of chromosomes 2 of sugarcane noble species and saccharum spontaneum and application of microsatellite molecular marker
  • Microsatellite molecular marker for distinguishing genetic backgrounds of chromosomes 2 of sugarcane noble species and saccharum spontaneum and application of microsatellite molecular marker

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Embodiment 1

[0040] 1. Searching for SSR sequences in the whole genome sequences of sugarcane noble species and cutshou dense species, and the design and verification of SSR primers

[0041] 1.1 SSR site scanning

[0042]The Perl script in the Micro Satellite identification tool-MISA software package was used to scan the SSR loci of the sugarcane genome in batches. The criteria for whether it is an SSR in this study are: 1) One unit has at least ten repeats, two units have at least seven repeats, At least six repetitions of three units, at least five repetitions of four units, at least four repetitions of five units, and at least four repetitions of six units. 2) When the distance between two SSRs is less than 100 bp, they are combined into a composite SSR.

[0043] 1.2 Sequence interception

[0044] Calculate the physical position of each SSR site on the genome sequence, use the SSR position information to intercept the flanking sequences of 150 bp before and after the SSR start and sto...

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Abstract

The present invention discloses a microsatellite molecular marker for distinguishing genetic backgrounds of chromosomes 2 of sugarcane noble species and saccharum spontaneum and an application of themicrosatellite molecular marker. The microsatellite molecular marker comprises four pairs of SSR molecular marker core primer pairs located on the chromosome 2 of the sugarcane noble species. Whole genome data based on the sugarcane noble species and saccharum spontaneum are studied, SSR marker primers are designed and synthesized, and polyacrylamide gel electrophoresis verification is performed on 5 noble species materials, 4 saccharum spontaneum materials, and 2 cultivated species materials. The selected 4 pairs of the primers have characteristics of stable amplification results and clear and identifiable electrophoretic bands, chromosomal positions of the sugarcane noble species and saccharum spontaneum where SSR is located can be distinguished, confirming that the SSR marker has relatively high repetitiveness and polymorphism and can be used for sugarcane genetic analysis and variety identification.

Description

technical field [0001] The invention belongs to the technical field of sugarcane molecular breeding, in particular to a microsatellite molecular marker for distinguishing the genetic background of No. 2 chromosome of sugarcane noble species and cut hands dense species and its application. Background technique [0002] sugar cane( Saccharum hybrids ) is an important sugar crop in the world and one of the main economic crops in southern my country. Sugarcane sugar production accounts for about 80% of the world's total sugar production. At the same time, among the by-products of sugarcane sugar extraction, bagasse contains about 50% of the fiber It can be used to make paper, and the cellulose in bagasse can be converted into sugar to make alcohol. 40% of the world's alcohol is produced from sugarcane. Saccharum ( Saccharum ) including tropical species ( S. officinarum ), Chinese species ( S. sinense ), Indian species ( S. barberi ), large stem wild species ( S. robust...

Claims

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Application Information

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IPC IPC(8): C12Q1/6895C12Q1/6809C12N15/11G16B20/30G16B30/10
CPCC12Q1/6895C12Q1/6809G16B20/30G16B30/10C12Q2600/156C12Q2531/113C12Q2565/125Y02E50/10
Inventor 张积森刘蕾王恒波王刚窦梅杰
Owner FUJIAN AGRI & FORESTRY UNIV
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