Automatic focusing device and automatic focusing method for changing image space optical path

Abstract

The invention relates to an automatic focusing device capable of changing an image space optical path, which comprises a computer, a motion controller, a three-axis motion platform, a microscope objective, a light splitting element, an optical path control element, a focusing camera and an image acquisition camera; the optical path control element enables known differences to exist in optical paths of four areas on a focusing camera sensor so as to generate defocusing of different degrees, wherein the out-of-focus degree of the image acquisition camera is different from the out-of-focus degrees of the four regions in the focusing camera; the focusing camera and the image acquisition camera communicate with the computer through signal lines; the computer communicates with the motion controller; and the motion controller communicates with the three-axis motion platform. The invention further discloses an implementation scheme of the automatic focusing method of the device, the current out-of-focus degree can be monitored in real time, real-time tracking focusing can be carried out during flying (motion acquisition), the acquisition efficiency, the system cost and the imaging qualityare considered to the maximum extent, and the system structure is compact.

Description

Technical field: [0001] The invention belongs to the technical field of microscope image scanning and processing, and relates to digital image processing technology and microscope optical path design technology. More specifically, the invention relates to an automatic focusing device for changing the optical path of the image side and an automatic focusing method for the device. Background technique: [0002] In the microscopic imaging system, the higher the resolution of the objective lens, the smaller the depth of field, especially the 20-100 times high-power microscope objective lens, the depth of field is about 1.5-0.5 microns. After the detected object is placed on the motion platform, the height difference of its surface often exceeds the depth of field of the imaging system. Therefore, when the imaging system scans and captures images of the entire surface of the detected object, it is necessary to adjust the focusing height of the imaging system in the Z direction to...

AI Technical Summary

Problems solved by technology

[0011] To sum up, when the microscope imaging system scans and acquires images, it is often difficult to take into account factors such as acquisition efficiency, system cost, imaging quality, and compact system structure.

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