Bacillus thuringiensis JXBT-0296 with insecticidal activity on ganoderma lucidum geometridae and application of bacillus thuringiensis JXBT-0296

A technology of Bacillus aureus and Bacillus, applied in the field of biological pesticides, can solve the problem of low insecticidal activity and achieve the effect of strong killing effect

Active Publication Date: 2020-09-18
江西省农业科学院农业应用微生物研究所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are relatively few strains that have insecticidal activity against the chimera pests, and the insecticidal activity is also relatively low

Method used

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  • Bacillus thuringiensis JXBT-0296 with insecticidal activity on ganoderma lucidum geometridae and application of bacillus thuringiensis JXBT-0296
  • Bacillus thuringiensis JXBT-0296 with insecticidal activity on ganoderma lucidum geometridae and application of bacillus thuringiensis JXBT-0296
  • Bacillus thuringiensis JXBT-0296 with insecticidal activity on ganoderma lucidum geometridae and application of bacillus thuringiensis JXBT-0296

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Isolation, screening, identification and preservation of Bacillus thuringiensis JXBT-0296:

[0027] 1. Isolation and Screening of Bacillus thuringiensis

[0028] The applicant collected a large number of soil samples from the jungle in the Lushan mountainous area of ​​Jiangxi Province, and used an innovative method to isolate and screen Bt strains. For specific methods, refer to the technical scheme with application number 201810402728.5. The screened Bt bacterial strains are numbered and named and preserved. The impure strains were isolated and purified by streaking on plates, and then preserved. The storage method is as follows: for long-term storage, use a glycerol tube to store at -80 °C, and for temporary storage, use an aqueous suspension of bacteria in the spore stage to store at 4 °C.

[0029] 2. Screening, identification and preservation of insecticidal active strains

[0030] 2.1 Screening of Bt strains with insecticidal activity against Ganoderma lucidum m...

Embodiment 2

[0044] Bioassay of Bacillus thuringiensis JXBT-0296 on important pests of Ganoderma lucidum:

[0045] 1. Preparation of Bioassay Fermentation Broth

[0046] In order to fully verify the insecticidal activity of Bacillus thuringiensis JXBT-0296 on the important pests of Ganoderma lucidum, three liquid fermentation media, LB, ICPM and TYS, were used to prepare bioassay samples by fermentation. The specific method was: in 250ml triangle Add 100 milliliters of liquid fermentation medium into the bottle, insert the spore suspension of Bacillus thuringiensis JXBT-0296 (spore concentration 1.00E+07) according to the inoculation amount of 1 / 100, and culture on a shaking table at 28° C. and 220 rpm.

[0047] The LB medium was cultivated for 12 hours. At this time, most of the thalli in the fermented liquid were in the growth stage of the vegetative phase, and the expression levels of insecticidal proteins and other insecticidal active ingredients in the vegetative phase were relatively...

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Abstract

The invention belongs to the field of biopesticides, and particularly relates to bacillus thuringiensis JXBT-0296 with insecticidal activity on ganoderma lucidum geometridae and application of the bacillus thuringiensis JXBT-0296. The separated and screened bacillus thuringiensis JXBT-0296 is preserved in the China Center for Type Culture Collection, the preservation number is CCTCC NO:M2020071, and the strain is separated from a soil sample collected from Lushan jungle in Jiangxi Province. Bioassay shows that a fermentation product of the strain has strong insecticidal activity on ganoderma lucidum geometridae, and also has repellent activity on ganoderma lucidum drosophila melanogaster; the strain also has an insecticidal effect on ganoderma lucidum noctuid, hapsitera barbata christoph,chilo suppressalis, plutella xylostella or cabbage caterpillar, and is particularly suitable for being developed into a biocontrol fungicide for preventing and treating ganoderma lucidum pests.

Description

technical field [0001] The invention belongs to the field of biological pesticides, and in particular relates to a bacillus thuringiensis JXBT-0296 having insecticidal activity against ganoderma lucidum moth and application thereof. Background technique [0002] Bacillus thuringiensis (Bt) is a rod-shaped bacterium that exists widely in nature. Its distinctive feature that distinguishes it from other bacillus is that it can produce parasporal crystals during the growth period of the spores. These parasporal crystals are harmful to many types of pests. Has insecticidal activity. In addition to parasporal crystals, substances with insecticidal activity produced by Bt also include: vegetative phase insecticidal protein, thuringin, synergistic protein, auxiliary protein, chitinase, collagenase, cytolysin, etc. (Malovichko Y.V., Nizhnikov A.A., Antonets K.S.Repertoire of the virulencefactors unrelated to major classes of protein toxins and its role inspecificity of host-pathogen...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/23A01P7/04C12R1/07
CPCA01N63/23C12N1/205C12R2001/07
Inventor 王奋山刘子荣徐雪亮范琳娟孙娅娟姚英娟
Owner 江西省农业科学院农业应用微生物研究所
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