Method for constructing unknown plant species identification database based on a matk gene and database

Abstract

A method for constructing an unknown plant species identification database based on a matk gene and the database are provided. The method comprises the following steps of: acquiring an original sequence data file containing a matk gene; extracting species annotation information in the original sequence data file; integrating the species annotation information to the matK gene to obtain a matK sequence; performing quality control on the matk sequence; clustering according to the similarity between the matk sequences; and constructing the database according to the clustering result. The construction method of the unknown plant species identification database based on the matk gene and the database have the advantages that: (1) the detection flux is high; (2) high sensitivity and selectivityare achieved; (3) the database constructed by the method has wide coverage, high data quality and comprehensive information; and (4) unknown species can be determined at the species level using bioinformatics methods.

Description

technical field [0001] The invention belongs to the field of plant species identification, and in particular relates to a construction method and database of an unknown plant species identification database based on matk gene. Background technique [0002] The matK gene exists in the chloroplast lysine trnK gene and is located in the intron between the two highly conserved exons. It is a single-copy coding gene and participates in the splicing of the coding transcript by the type II intron The mature enzyme, whose evolution rate is between rbcL and ITS, has a high ability to identify species at the family and genus levels. In 2009, the matK gene and the rbcL gene were recommended by the Consortium for Biological Barcodes (CBOL) as the core DNA barcodes for plant DNA barcode-related research based on the criteria of sequencing quality, species identification efficiency and versatility. [0003] Although the matk gene has excellent species identification efficiency, versatili...

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Problems solved by technology

[0007] The invention provides a construction method and database of an unknown plant species identification database based on the matK gene, which solves the problem in the prior art that there is still a lack of an accurate, efficient, and large-coverage database when identifying unknown plant species

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