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Suction head assembly with filter element

A technology of suction head and components, which is applied in the direction of sterilization method, recombinant DNA technology, method of supporting/immobilizing microorganisms, etc., can solve the problems such as contamination of filter tip and affecting the purity of nucleic acid extraction, so as to ensure the purity and avoid Contamination, cost reduction effect

Pending Publication Date: 2020-09-22
陈辉
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In the suction method, the suction head generally includes a connecting suction pipe connected to a suction source and a filter tip sleeved at the lower end of the connecting suction tube. The filter tip is provided with a nucleic acid component that can specifically adsorb the sample. After the suction operation, there is a risk of contamination of the filter tip due to the contamination of the connecting pipette by the flowing sample, which affects the purity of nucleic acid extraction.

Method used

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  • Suction head assembly with filter element
  • Suction head assembly with filter element
  • Suction head assembly with filter element

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Embodiment Construction

[0022] The present invention will be further described in detail below in conjunction with the accompanying drawings and embodiments.

[0023] Such as Figure 1~5 As shown, a suction head assembly with a filter element includes a connecting suction tube 91 , a filter tip 83 and a disposable capillary suction tube 81 . Wherein, the upper port of the above-mentioned connecting suction pipe 91 is used to connect with the suction source. In this embodiment, the suction source can be a vacuum pump, and the lower port is inserted into the upper end of the filter tip 83, and the lower end of the filter tip 83 is embedded with an adsorption Filter element 830. The upper end of the disposable capillary pipette 81 is sleeved on the lower end of the above-mentioned connecting pipette 91 and embedded in the upper port of the filter tip 83, and the disposable capillary tube 81 is located in the above-mentioned filter tip 83 and located in the above-mentioned adsorption filter element 830 ...

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Abstract

The invention relates to a suction head assembly with a filter element. The suction head assembly comprises a connecting suction pipe and a filter element suction head, the upper port of the connecting suction pipe is used for being connected to a suction source, the lower port of the connecting suction pipe is inserted into the upper end of the filter element suction head, an adsorption filter element is embedded into the lower end of the filter element suction head, the suction head assembly also comprises a disposable capillary suction pipe, the upper end of the disposable capillary suctionpipe sleeves the lower end of the connecting suction pipe, and the disposable capillary suction pipe is located in the filter element suction head. By arranging the disposable suction pipe, the connecting suction pipe and the filter element suction head are separated through the unpolluted disposable capillary suction pipe, and the connecting suction pipe is prevented from being in contact with the filter element suction head, so that the filter element suction head can be prevented from being polluted, and the purity of nucleic acid extraction is ensured. In addition, the disposable capillary pipe enables the solution to be rapidly and completely pumped away from the filter element through the capillary effect, and therefore thorough separation of nucleic acid and a liquid adsorbed on the filter element and thorough cleaning of impurities on the filter element are guaranteed.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a suction head assembly with a filter core. Background technique [0002] Liquid suction is a routine operation in biological experiments, and the suction head is one of the necessary tools to realize the liquid suction operation. Nucleic acid extraction is a very important technology in life science research, biotechnology application and gene diagnosis. At present, commercial nucleic acid extraction methods are mainly based on solid-phase adsorption methods, that is, nucleic acid molecules are adsorbed on the surface of a certain solid-phase adsorbent under specific conditions (such as pH, specific salt, etc.), and then eluted. Purification method. To achieve the above operations, centrifugation or suction can be used. In the suction method, the suction head generally includes a connecting suction pipe connected to a suction source and a filter tip sleeved at the lower end of th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/12C12M1/00
CPCC12N15/1017
Inventor 陈辉
Owner 陈辉