Bacillus velezensis as well as application and preparation thereof

A technology of Bacillus Velez and Bacillus, applied in the direction of application, fungicides, chemicals for biological control, etc., can solve the problems of secondary pollution of medicinal materials and pesticides, damage to soil ecological balance, residues, etc., to prevent invasion Astragalus root, inhibit germination, reduce the effect of using

Active Publication Date: 2020-09-22
NORTHWEST A & F UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Pesticide control of Astragalus root rot will destroy the ecological balance of the soil,

Method used

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  • Bacillus velezensis as well as application and preparation thereof
  • Bacillus velezensis as well as application and preparation thereof
  • Bacillus velezensis as well as application and preparation thereof

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Experimental program
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Embodiment Construction

[0021] The present invention will be described in detail below in conjunction with specific embodiments.

[0022] (1) Soil sample collection:

[0023] In August 2018, root samples were collected from the Astragalus planting base in Dangchang County, Gansu Province. The loose soil on the roots of Astragalus was removed, and the complete plants were placed in sterile ziplock bags, and all samples were frozen in ice packs and sent back to the laboratory. The sampling site has an average annual rainfall of 530mm and an altitude of 2300m.

[0024] (2) Isolation of Astragalus endophytes:

[0025] Cut the roots of Astragalus membranaceus into small sections of appropriate length with sterile scissors, clean the soil on the root surface with a soft brush, rinse thoroughly with sterile water, and absorb the water on the root surface with absorbent paper. Soak the roots with 75% ethanol for 2 minutes, rinse the residual ethanol with sterile water, then soak in 1% sodium hypochlorite f...

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Abstract

The invention discloses bacillus velezensis as well as application and a preparation thereof. The preservation number of the bacillus velezensis in China Center for Type Culture Collection is CCTCC No:M2020218. The strain can effectively inhibit germination of pathogenic bacteria F.tricinctum spores, and the bacillus velezensis, fermentation liquor and/or metabolite have/has a remarkable effect inpreventing and treating astragalus root rot and promoting growth.

Description

technical field [0001] The invention relates to the technical field of biological bacteria agents, in particular to a strain of bacillus velei and its application and preparation in preventing and controlling astragalus root rot and promoting growth. Background technique [0002] Astragalus is one of the traditional bulk medicinal materials in my country. With the increase of market demand, the crop rotation cycle in the cultivation process of Astragalus becomes shorter, coupled with global warming, the frequency of root rot of Astragalus increases significantly, seriously affecting the The yield and quality of Radix Astragali (Gao Fen et al., 2018; Xing Zhanmin et al., 2015). Therefore, how to economically and efficiently prevent the occurrence of Astragalus root rot has attracted the attention of many scholars. Pesticide control of Astragalus root rot will destroy the ecological balance of the soil, easily cause secondary pollution and cause pesticide residues in medicinal...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/22A01P3/00A01P21/00A01G22/25A01G7/06C12R1/07
CPCC12N1/20A01N63/22A01G22/25A01G7/06C12R2001/07C12N1/205
Inventor 韦革宏李哲斐白晓丽
Owner NORTHWEST A & F UNIV
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