Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for improving antibacterial activity of antibacterial peptide BSN-37

A technology of BSN-37 and PUC-BSN-37 is applied in the field of improving the bacteriostatic activity of the antibacterial peptide BSN-37, which can solve the problems of low solubility, high toxicity, poor antibacterial effect, etc., and achieves low preparation cost, reduced toxicity, Improve the effect of bactericidal action

Inactive Publication Date: 2020-09-22
广州颜如玉生物科技有限公司
View PDF1 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] In summary, the existing antimicrobial peptides generally have the disadvantages of low solubility and high toxicity, which easily lead to poor antibacterial effect

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for improving antibacterial activity of antibacterial peptide BSN-37

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Embodiment 1 A kind of method that improves antimicrobial peptide BSN-37 antibacterial activity

[0040] The method for improving antimicrobial peptide BSN-37 antibacterial activity comprises the following steps:

[0041] S1. Construct the plasmid PUC-BSN-37 containing the BSN-37 gene sequence;

[0042] According to the amino acid sequence and the characteristics of Escherichia coli preferred codons, its gene fragments were reverse-translated, and the gene of BSN-37 was synthesized by adding restriction endonucleases BamH I and Xho I, GST tags, terminators and protective bases, The size is 126bp, and it is cloned in the PUC57 vector, that is.

[0043] S2. Introducing the plasmid PUC-BSN-37 constructed in step S1 into the empty vector pCold-SUMO-thioredoxin to obtain a recombinant plasmid;

[0044] (1) Design primers for amplifying the BSN-37 gene sequence according to the enzyme cutting sites BamH I and Xho I, and use PCR technology to amplify the target fragment, seq...

Embodiment 2

[0052] Embodiment 2 A kind of method that improves antimicrobial peptide BSN-37 antibacterial activity

[0053] The method for improving the antimicrobial peptide BSN-37 antibacterial activity is similar to Example 1;

[0054] The difference from Example 1 is that the mixture added in step S3 in Example 2 is composed of glucocorticoid and Danshen extract in a mass ratio of 2:9, and the added amount is 0.15% by weight.

Embodiment 3

[0055] Embodiment 3 A kind of method that improves antimicrobial peptide BSN-37 antibacterial activity

[0056] The method for improving the antimicrobial peptide BSN-37 antibacterial activity is similar to Example 1;

[0057] The difference from Example 1 is that the mixture in Example 3 is composed of glucocorticoid and Danshen extract in a volume ratio of 1.5:8, and the added amount is 0.13% of the total volume.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention belongs to the field of bioengineering, and particularly relates to a method for improving antibacterial activity of antibacterial peptide BSN-37. The preparation method mainly comprisesthe following steps that SUMO and thioredoxin blank vectors and antibacterial peptide vectors are correspondingly constructed, and then double enzyme digestion is correspondingly conducted on the blank vectors and antibacterial peptide BSN-37 by utilizing restriction enzymes BamH I and Xho I to prepare recombinant vector plasmids, so as to achieve the purposes of improving the solubility of the antibacterial peptide and reducing the toxicity. According to the method for improving the antibacterial activity of the antibacterial peptide BSN-37, the finally obtained antibacterial peptide is lowin toxicity, high in solubility and high in antibacterial activity.

Description

technical field [0001] The invention belongs to the field of bioengineering, and in particular relates to a method for improving the antibacterial activity of antibacterial peptide BSN-37. Background technique [0002] Antimicrobial peptides (AMPs) are a type of small molecular active peptides produced by animal bodies when resisting the invasion of foreign microorganisms. Since most antimicrobial peptides are small peptides, the amino acid sequence is relatively short, generally about 50. Among these amino acids, hydrophobic ones account for about 50%, and the antibacterial activity is stronger due to the excess of basic residues and a net positive charge. The main advantages of antimicrobial peptides are: first, they have broad-spectrum antibacterial activity and good stability; second, they mainly target the microbial membrane of the body, which can prevent microorganisms from developing drug resistance to them. Therefore, these peptides are considered as promising cand...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N15/31C12N15/70
CPCC07K14/245C07K2319/35C07K2319/95C12N15/70
Inventor 王中振谢涛
Owner 广州颜如玉生物科技有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com