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A Minimalist Laser Microscope Based on 3D Specimen Lens Integrator

A laser microscope and specimen technology, which is applied in the fields of education and experimental testing, can solve the problem of inability to achieve 360-degree three-dimensional observation, and achieve the effects of convenient operation and observation, easy portability and ingenious design.

Active Publication Date: 2022-07-15
沈天童
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Due to the operations such as pressing and slicing in the production process of microscopic slide specimens, only flat two-dimensional observation of biological specimens can be carried out, and it is impossible to realize all-round and 360-degree three-dimensional observation of biological specimens under a microscope. greater limitations

Method used

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  • A Minimalist Laser Microscope Based on 3D Specimen Lens Integrator
  • A Minimalist Laser Microscope Based on 3D Specimen Lens Integrator
  • A Minimalist Laser Microscope Based on 3D Specimen Lens Integrator

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] The structure of the minimalist laser microscope based on the three-dimensional specimen lens integrated body provided in this embodiment is as follows: figure 1 As shown, it includes a three-dimensional specimen lens integrated body 1 , a three-dimensional stage 2 and a micro-laser lamp 3 .

[0031] The three-dimensional specimen lens integrated body 1 includes a spherical transparent body 11 and a specimen 12. The spherical transparent body 11 is a spherical lens with a diameter of 1-15 mm, a diameter tolerance of ≤ 0.05 mm, and a light transmittance of ≥ 96%. In this embodiment, the diameter of the spherical lens is 6.0 mm, the light transmittance is 98%, and the diameter tolerance is 0.01 mm. The material of the spherical lens is glass; the specimen 12 includes solid specimens such as animals, plant specimens and natural materials. like Figure 4 As shown, the specimen 12 is permanently bonded to the surface of the spherical lens by a high light transmission glue ...

Embodiment 2

[0047] The difference from Example 1 is that the bonding method between the specimen 12 and the spherical lens (spherical transparent body 11 ) in the three-dimensional specimen lens integrated body is different. In this embodiment, the specimen 12 is temporarily bonded to the spherical lens. , resulting in different fabrication, preservation and maintenance of 3D specimen lens integration.

[0048] The manufacturing method of the three-dimensional specimen lens integrated body is as follows:

[0049] Take a glass spherical lens with a diameter of 6.0mm, wash the spherical lens with pure water, dry it for later use, use an elastic clamp to clamp the clean and dust-free spherical lens, place the spherical lens on a clean operating table, use a needle or a syringe A drop of water is placed on the center of the top surface of the spherical lens, and then the specimen is picked up with a needle, and the specimen is sealed and immersed in the drop of water;

[0050] The preservati...

Embodiment 3

[0053] The difference from Example 1 is that the structure of the three-dimensional specimen lens integrated body is different, such as Figure 5 As shown, in this embodiment, the specimen 12 is covered with a cover glass 15, and the manufacturing method of the three-dimensional specimen lens integrated body in this embodiment is as follows:

[0054] Take a glass spherical lens with a diameter of 6.0mm, wash the spherical lens with pure water, and dry it for later use. Use the elastic clamp of the three-dimensional stage to clamp the clean and dust-free spherical lens, and place the spherical lens on a clean operating table. , drop photosensitive adhesive droplets on the center of the top surface of the spherical lens, the photosensitive adhesive droplets keep as regular spherical shape as possible on the surface of the spherical lens, and then pick up the specimen with a needle, seal the specimen and immerse it in the photosensitive adhesive droplet, and then put it on the spe...

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Abstract

The invention discloses a minimalist laser microscope based on a three-dimensional specimen lens integrated body, comprising a three-dimensional specimen lens integrated body, a three-dimensional object stage and a miniature laser lamp. The three-dimensional specimen lens integrated body includes a spherical transparent body and a specimen, and the specimen is fixed on a On the surface or inner center of the spherical transparent body, the three-dimensional stage is connected with the three-dimensional specimen lens integrated body, and the three-dimensional specimen lens integrated body can rotate and move up and down 360°. The laser microscope has two functions of fixed preservation of three-dimensional biological specimens and three-dimensional microscopic magnification observation of specimens, and has an ingenious design, ultra-small size, no need to focus during use, convenient use and display, and extremely low production cost.

Description

technical field [0001] The invention relates to the technical fields of education and experimental detection, in particular to a minimalist laser microscope based on a three-dimensional specimen lens integrated body, which is suitable for the fixed preservation of three-dimensional biological specimens, the three-dimensional microscopic magnification observation, and the multi-sample analysis. People observe microscopically at the same time. Background technique [0002] Currently, the commonly used microscope is an optical microscope, which has disadvantages such as complex structure, bulky size, complicated operation, and easy damage. In the process of observing biological specimens with an optical microscope, a series of complex operations such as precise optical focusing, adjusting the light entering conditions of the microscope, and adjusting the observation field are required, which requires high technical level of the observers. In addition, the currently commonly us...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G02B21/00G02B21/06G02B21/26G02B21/34G02B21/36G01N21/84G01N21/01
CPCG02B21/0008G02B21/06G02B21/26G02B21/34G02B21/364G02B21/361G01N21/84G01N21/01
Inventor 沈天童
Owner 沈天童
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