Identification method of phosphorus activation efficiency of peanut germplasm under hydroponic conditions
An identification method, a technology of phosphorus activation, applied in the field of agricultural science, can solve the problems that it is difficult to identify the activation efficiency of insoluble phosphorus in plants, and there is no identification method for the activation efficiency of phosphorus in peanut germplasm, so as to achieve good root system integrity, Identify the exact effect
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Embodiment 1
[0022] A method for identifying the phosphorus activation efficiency of peanut germplasm under hydroponic conditions of the present invention comprises the following steps:
[0023] Step 1: the cultivation of peanut seedlings;
[0024] Step 1.1: Seed preparation, select a few peanut seeds that have passed the dormancy period, are uniform in size and have a complete seed coat, soak the seeds in alcohol and disinfect them with 0.1% H 2 o 2 Soak the seeds in solution for 8-12 hours, and keep in a dark environment during the soaking period. Hydrogen peroxide can disinfect and accelerate germination, but the soaking time should not be too long, otherwise it will not be conducive to seed germination. Preferably, the alcohol concentration in this step is between 70% and 80%, and the soaking and disinfection time is between 27 and 33 seconds, and if the time is too long, it will cause damage to the peanuts.
[0025] Step 1.2: Preparation of the germination box, clean the inside and ...
Embodiment 2
[0040] A method for identifying phosphorus activation efficiency of peanut germplasm under hydroponic conditions. During the seed germination period and the entire cultivation period, the temperature of the growth chamber is controlled at 24°C, the light intensity is 7000LX, and the light is 17h per day. Specific steps are as follows:
[0041] Step 1: the cultivation of peanut seedlings;
[0042] Step 1.1: Seed preparation, select 120 peanut seeds that have passed the dormant period, are uniform in size and have a complete seed coat, soak and disinfect the seeds in 75% alcohol for 30 seconds, and then wash them with 0.1% H 2 o 2 Soak the seeds in the solution for 10 hours, and keep the dark environment during the soaking period.
[0043] Step 1.2: Preparation of the germination box, clean the inside and outside of the germination box, wet the bottom of the germination box with deionized water, so that the inner wall of the germination box can be tightly attached to the plast...
Embodiment 3
[0058] Before the identification of phosphorus activation efficiency, the identification of phosphorus absorption efficiency can be carried out first, which is beneficial to realize the identification of phosphorus absorption and phosphorus activation efficiency by cultivating peanut plants once, which not only saves the cultivation time of peanut plants, but also improves the identification efficiency . In this example, on the basis of Example 1 or Example 2, after step 2.1 and before step 2.2, the phosphorus absorption efficiency is identified, and the specific operations are as follows:
[0059] Sampling and measurement, first move the plants into deionized water and starve for more than 20 hours, then immerse all the roots in the absorption solution containing P concentration of 0.2mmol / L. H in solution 2 PO 4 Concentration changes, using the formula: Ip=(Q 1 -Q 2 ) / (h*N) to get the average absorption rate (μmol / h / strain) of this absorption stage, where, Q 1 : phospho...
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