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Oligopeptide, weight loss composition, preparation method and application

A composition and oligopeptide technology applied in the biological field to achieve the effect of inhibiting the activity of pancreatic lipase

Active Publication Date: 2022-05-03
完美(广东)日用品有限公司 +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Although it has been reported that the phenolic substances extracted from the fruit extract of Salmonella chinensis have pancreatic lipase inhibitory activity; See report on oligopeptide compounds with pancreatic lipase inhibitory activity

Method used

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  • Oligopeptide, weight loss composition, preparation method and application
  • Oligopeptide, weight loss composition, preparation method and application
  • Oligopeptide, weight loss composition, preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] This embodiment provides a weight loss composition containing Ala-Pro-Tyr-Arg (APYR) oligopeptide, the preparation method of which is as follows:

[0053] Grind seabuckthorn seeds to 100 mesh and mix them with water at a mass ratio of 1:10 to obtain an aqueous solution of seabuckthorn seeds, adjust its pH to 6.5, and then add neutral protease (the mass ratio of neutral protease to crushed seabuckthorn seeds is 0.25:100 ), then stirred and hydrolyzed at 37°C for 2 hours, and then inactivated the enzyme at 95°C for 15 minutes after the hydrolysis to obtain the seabuckthorn seed protein hydrolyzate;

[0054]Centrifuge the above seabuckthorn seed protein hydrolyzate at 4°C and 8000 rpm for 20 minutes, and then take the supernatant and freeze-dry to obtain the weight loss composition containing APYR oligopeptide. As detected by HPLC, the content of APYR oligopeptide in the weight loss composition is 1.85g / 100g.

[0055] This embodiment also provides a method for purifying A...

Embodiment 2

[0063] This embodiment provides a weight loss composition containing Ala-Pro-Tyr-Arg (APYR) oligopeptide, the preparation method of which is as follows:

[0064] Pulverize the seabuckthorn seeds to 100 mesh and mix them with water at a mass ratio of 1:15 to obtain an aqueous solution of seabuckthorn seeds, adjust the pH to 5.0 and add trypsin (the mass ratio of pancreatin to crushed seabuckthorn seeds is 1.0:100), Then stir and hydrolyze at 25°C for 24 hours, and inactivate the enzyme at 85°C for 10 minutes after the hydrolysis to obtain the seabuckthorn seed protein hydrolyzate;

[0065] Centrifuge the above seabuckthorn seed protein hydrolyzate at 3°C ​​and 10,000 rpm for 10 minutes, then take the supernatant and freeze-dry to obtain the weight loss composition containing APYR oligopeptide. As detected by HPLC, the content of APYR oligopeptide in the weight loss composition is 1.18g / 100g.

[0066] This embodiment also provides a method for purifying Ala-Pro-Tyr-Arg (APYR) o...

Embodiment 3

[0072] This embodiment provides a weight loss composition containing Ala-Pro-Tyr-Arg (APYR) oligopeptide, the preparation method of which is as follows:

[0073] After pulverizing seabuckthorn seeds to 100 meshes, mix them with water at a mass ratio of 1:12 to obtain an aqueous solution of seabuckthorn seeds, adjust its pH to 7.0, and then add pancreatin (the mass ratio of pancreatin to pulverized seabuckthorn seeds is 2.0:100 ), then stirred and hydrolyzed at 60°C for 15 hours, and inactivated the enzyme at 90°C for 20 minutes to obtain seabuckthorn seed protein hydrolyzate;

[0074] Centrifuge the above seabuckthorn seed protein hydrolyzate at 5°C and 6000 rpm for 15 minutes, then take the supernatant and freeze-dry to obtain the weight loss composition containing APYR oligopeptide. As detected by HPLC, the content of APYR oligopeptide in the weight loss composition is 1.02g / 100g.

[0075] This embodiment also provides a method for purifying Ala-Pro-Tyr-Arg (APYR) oligopept...

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Abstract

The invention provides an oligopeptide, a weight loss composition, a preparation method and an application. Wherein, the oligopeptide has the following amino acid sequence: Ala-Pro-Tyr-Arg. The oligopeptide has the effect of inhibiting the activity of pancreatic lipase, is natural, safe, has no toxic and side effects, and can be taken for a long time.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to an oligopeptide, a weight loss composition, a preparation method and an application. Background technique [0002] With the continuous improvement of people's material life, unhealthy high-fat diet and bad living habits induce the occurrence of various chronic diseases. Excessive intake of lipids and severe lack of exercise lead to fat deposition in various parts of the body, which gradually evolves into obesity. Obesity is related to many chronic diseases, such as diabetes, fatty liver and cardiovascular diseases. The fat in the food needs to be hydrolyzed by the corresponding digestive enzymes in the gastrointestinal tract before it can be absorbed by the body. About 70% of the fat in the diet is composed of pancreatic fat Enzymes perform hydrolytic digestion. Therefore, by inhibiting the activity of pancreatic lipase, reducing and delaying postprandial fat hydrolysis and absorpt...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K5/103C07K1/36C07K1/34C07K1/18C12P21/06A61K38/07A61P3/04A23L33/18A23L33/105
CPCC07K5/1008C12P21/06A61P3/04A23L33/18A23L33/105A61K38/00A23V2002/00A23V2200/332A23V2250/21A23V2250/55
Inventor 叶健文崔春刘洪霞孙允武杜玉兰孙忠伟李家炜严建刚李晓敏古润金
Owner 完美(广东)日用品有限公司