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Achyranthes bidentata polysaccharide as well as preparation method and application thereof

A polysaccharide of Achyranthes and Achyranthes giganta are applied in the field of polysaccharide of Achyranthes giganta, which can solve the problems of low polysaccharide content and complex composition of total polysaccharides, and achieve the effects of simple preparation method, convenient process expansion and mild reaction conditions.

Active Publication Date: 2022-04-01
CHINA PHARM UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Purpose of the invention: The present invention aims at the problem of complex composition of total polysaccharides and low polysaccharide content in the prior art, and provides a kind of Achyranthes bidentata polysaccharide with clear chemical composition and high purity , also relates to its preparation method and application

Method used

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  • Achyranthes bidentata polysaccharide as well as preparation method and application thereof
  • Achyranthes bidentata polysaccharide as well as preparation method and application thereof
  • Achyranthes bidentata polysaccharide as well as preparation method and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0030] Preparation method of refined Achyranthes bidentata polysaccharide (AB)

[0031] After pulverizing the Achyranthes bidentata medicinal material, weigh 500g of medicinal material powder and add 5 times of absolute ethanol, condense and reflux extraction at 80-90°C for 3 times, each time for 2 hours, remove small molecular impurities, filter, collect medicinal residues, and dry at 50°C. Add 10 times the volume of distilled water, condense and reflux at 70-80°C for 3 times, each time for 2 hours, filter the combined filtrate, concentrate under reduced pressure at 45-55°C to about 1.0L, add 4 times the volume of absolute ethanol to precipitate, and stand overnight at 4°C , remove the upper clarified solution to collect the precipitate, wash with absolute ethanol, acetone, and absolute ether successively, add an appropriate amount of distilled water to dissolve the polysaccharide precipitate, then use the Savage method to repeatedly deproteinize, collect the Achyranthes biden...

Embodiment 2

[0033] Preparation method of Achyranthes bidentata homogeneous polysaccharide AB-1-1

[0034] Weigh 3.0 g of Achyranthes bidentata polysaccharide dry powder and add appropriate amount of distilled water to prepare a saturated solution, conduct primary separation through DEAE-FF column (60×2.6cm), and carry out gradient washing with distilled water, 0.1, 0.2, 0.3, 0.4, 0.5M NaCl solution successively Elution, elution flow rate 2.0-3.0mL / min, automatic collection at 40-50mL / T, color development by sulfuric acid-anthrone method, ultraviolet detection in the range of 620-630nm, the absorbance value is the ordinate, and the number of collection tubes is the abscissa Draw the elution curve, collect the samples according to the elution curve, and freeze-dry; the components eluted with NaCl solution need to be dialyzed through the dialysis bag for 48 hours and then freeze-dried.

[0035] Take 400 mg of the above freeze-dried Achyranthes bidentata polysaccharide powder, dissolve in 2.0...

Embodiment 3

[0037] Determination of physicochemical properties of Achyranthes bidentata homogeneous polysaccharide AB-1-1

[0038] 1. Determination of purity and molecular weight

[0039] High performance gel permeation chromatography: Agilent 1290HPLC system equipped with G4260BELSD detector, AgilentPL aquagel-OH MIXED-H, Agilent PL aquagel-OH MIXED-M (300×7.5mm, 8um) series column, 25℃, 0.6mL / min ultra Pure water mobile phase detection, sample concentration 1.0mg / mL, injection 10uL, detection time 40min, such as figure 2 As shown, the results show that AB-1-1 is a high-purity homogeneous polysaccharide;

[0040]The SEC-MALS system was used to measure the weight average molecular weight, number average molecular weight and intrinsic viscosity of AB-1-1, 1200HPLC combined with MALS, ViscoStar, dRI detector, Shodex OHpak-806M HQ chromatographic column, physiological saline as mobile phase, sample concentration 4.5 mg / mL, inject 100uL. Such as image 3 and Figure 4 As shown, the resu...

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Abstract

The invention discloses achyranthes root homogeneous polysaccharide AB-1-1, which consists of fructose, glucose, mannose, galactose and arabinose, is formed by connecting five glucosidic bonds, namely T-alpha-D-Glcp,-> 1)-beta-D-Fruf-(2->,-> 2)-beta-D-Fruf-(6->,-> 1, 6)-beta-D-Fruf-(2-> and beta-D-Fruf-(2->, and has the weight-average molecular weight of 3.998 * 10 < 3 > Da (+ / -3.936%) and the intrinsic viscosity of 4.34 mL / g (+ / -2.03%). The preparation method of the homogeneous polysaccharide comprises the steps of polysaccharide extraction, deproteinization and freeze-drying, column separation and elution and drying. The invention also provides an application of the homogeneous polysaccharide AB-1-1 in preparation of pancreatic lipase activity inhibition drugs and weight-losing drugs. The homogeneous polysaccharide disclosed by the invention is clear in chemical composition, small in toxic and side effects and remarkable in pancreatic lipase inhibition activity.

Description

technical field [0001] The invention relates to the field of natural medicine and chemistry, in particular to a polysaccharide from Achyranthes bidentata, and also relates to its preparation method and application. Background technique [0002] Obesity has become a global health problem characterized by increased fat mass, adipocyte hyperplasia and hypertrophy leading to adipose tissue dysfunction. The fat eaten by mammals is generally digested and absorbed through the hydrolysis of pancreatic lipase. Pancrelipase is a key enzyme in the process of fat hydrolysis, which can catalyze the hydrolysis of natural substrate oil into glycerol and fatty acids. Inhibiting its activity can effectively inhibit the hydrolysis and Absorption, so as to achieve the effect of controlling and treating obesity; for example, Orlistat, a semi-synthetic pancreatic lipase inhibitor drug, has been used in clinical treatment of obesity, but the treatment process is accompanied by side effects such a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00A61K31/715A61K36/21A61P3/04A61K125/00
Inventor 闻晓东司华阳陈依梦杨杰
Owner CHINA PHARM UNIV