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A kind of oligopeptide, weight loss composition and preparation method and application

A composition and oligopeptide technology, applied in the biological field, to achieve the effect of improving and inhibiting the activity of pancreatic lipase

Active Publication Date: 2022-02-01
完美(广东)日用品有限公司 +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Seabuckthorn was originally wild, and it has been widely planted in recent years for soil conservation and sand fixation. At present, there is no report on the preparation of seabuckthorn protein-enzymatic hydrolysis products rich in pancreatic lipase inhibitory activity.

Method used

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  • A kind of oligopeptide, weight loss composition and preparation method and application
  • A kind of oligopeptide, weight loss composition and preparation method and application
  • A kind of oligopeptide, weight loss composition and preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] This example provides a weight loss composition containing Glu-Glu-Ala-Ala-Ser-Leu-Arg (EEAASLR) oligopeptide, the preparation method of which is as follows:

[0053] After pulverizing seabuckthorn seeds to 100 mesh, mix them with water according to the mass ratio of 1:10 to obtain an aqueous solution of seabuckthorn seeds, adjust its pH to 10.0, and then add alkaline protease (the mass ratio of the alkaline protease to crushed seabuckthorn seeds is 0.25 : 100), and then stirred and hydrolyzed at 50°C for 8h, and inactivated the enzyme at 90°C for 20min after the hydrolysis, to obtain the seabuckthorn seed protein hydrolyzate;

[0054] Centrifuge the above seabuckthorn seed protein hydrolyzate at 4°C and 8000 rpm for 20 minutes, then take the supernatant and freeze-dry to obtain the weight loss composition containing EEAASLR oligopeptide. After testing, the content of EEAASLR oligopeptide in the weight loss composition is 1.35g / 100g.

[0055] This embodiment also provide...

Embodiment 2

[0062] This example provides a weight loss composition containing Glu-Glu-Ala-Ala-Ser-Leu-Arg (EEAASLR) oligopeptide, the preparation method of which is as follows:

[0063] After pulverizing the seabuckthorn seeds to 100 mesh, mix them with water at a mass ratio of 1:10 to obtain an aqueous solution of seabuckthorn seeds, adjust its pH to 9.0, and then add a compound protease (the mass ratio of the compound protease to the crushed seabuckthorn seeds is 0.5:100 ), and then stirred and hydrolyzed at 50°C for 24 hours, and then inactivated the enzyme at 90°C for 20 minutes after the hydrolysis to obtain the seabuckthorn seed protein hydrolyzate;

[0064] Centrifuge the above seabuckthorn seed protein hydrolyzate at 4°C and 8000 rpm for 10 minutes, then take the supernatant and freeze-dry to obtain the weight loss composition containing EEAASLR oligopeptide. After testing, the content of EEAASLR oligopeptide in the weight loss composition is 0.68g / 100g.

[0065] This embodiment ...

Embodiment 3

[0070] This example provides a weight loss composition containing Glu-Glu-Ala-Ala-Ser-Leu-Arg (EEAASLR) oligopeptide, the preparation method of which is as follows:

[0071] After pulverizing the seabuckthorn seeds to 100 mesh, mix them with water according to the mass ratio of 1:10 to obtain an aqueous solution of seabuckthorn seeds, adjust its pH to 9.0, and then add alkaline protease and composite protease (the alkaline protease, composite protease and pulverized seabuckthorn The mass ratio of the seeds is 0.25:025:100), then stirred and hydrolyzed at 60°C for 6 hours, and after the hydrolysis, the enzyme was extinguished at 90°C for 20 minutes to obtain the protein hydrolyzate of seabuckthorn seeds;

[0072] Centrifuge the above seabuckthorn seed protein hydrolyzate at 4°C and 8000 rpm for 10 minutes, then take the supernatant and freeze-dry to obtain the weight loss composition containing EEAASLR oligopeptide. After testing, the content of EEAASLR oligopeptide in the weig...

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Abstract

The invention provides an oligopeptide, a weight loss composition, a preparation method and an application. Wherein, the oligopeptide has the following amino acid sequence: Glu-Glu-Ala-Ala-Ser-Leu-Arg. The oligopeptide has the effect of inhibiting the activity of pancreatic lipase, is natural, safe, has no toxic and side effects, and can be taken for a long time.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to an oligopeptide, a weight loss composition, a preparation method and an application. Background technique [0002] In recent years, obesity has become the primary health problem of human beings. Obesity can not only cause cardiovascular diseases such as diabetes, hypertension, dyslipidemia and coronary heart disease, but also cause some chronic diseases and even mental diseases, resulting in a decrease in quality of life. Genetics, diseases, and excess energy can all lead to the occurrence of obesity, especially the intake of dietary fat is more likely to increase the risk of obesity. Therefore, controlling the intake or absorption of dietary fat can effectively control obesity. [0003] Food fat needs to be hydrolyzed by lipase before it can be absorbed. The main reason is that most triglycerides in the diet can only be absorbed by the body after being degraded into diglycerides, m...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K7/06C12P21/06C07K1/36C07K1/34C07K1/16A23L33/18A61K38/08A61P3/04
CPCC07K7/06C12P21/06A23L33/18A61P3/04A23V2002/00A61K38/00A23V2200/332A23V2250/55
Inventor 叶健文崔春欧阳道福孙允武魏毅凡喻亮陈石生喻勤李晓敏古润金
Owner 完美(广东)日用品有限公司