Application of prinsepia utilis royle peel and pulp extract
A green thorn fruit, extract technology, applied in the field of food or medicine, to achieve broad market prospects, inhibition of pancreatic lipase activity, simple extraction and preparation process
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Embodiment 1
[0015] fresh green thorn fruit ( P. utilis Royle ) after removing the seeds, homogenate the pulp and peel; the crushed homogenate (50g) is mixed with formic acid-methanol (containing 1% formic acid and 80% methanol), the ratio of solid to liquid g:mL is 1:5, Ultrasonic extraction in a water bath for 30 minutes, ultrasonic power 210W, ultrasonic temperature 35°C, filtered to obtain the extract, and the filter residue was extracted twice, combined with the extract, and then concentrated under reduced pressure at 40°C to remove the solvent; the concentrate was placed on an Amberlite XAD-7HP column, The column was washed with 1% aqueous formic acid to remove non-polyphenolic substances, and the sample was eluted with pure methanol. The methanol eluate was collected, concentrated, and freeze-dried to obtain the total phenolic component, sample 1. Take a part of the total phenolic component and dissolve the sample with deionized water of pH 7, put it on a SephadexLH-20 column, wash...
Embodiment 2
[0017] Determination of the total phenolic content in each component sample of the green thorn fruit: dissolve the freeze-dried sample with a mass concentration of 80% methanol solution to make a 1mg / mL sample solution, take 1 mL and add it to a 10 mL centrifuge tube, then add 6 mL of distilled water and 0.5mL folinol, mix well and add 1.5mL 20% Na 2 CO 3 solution, and finally dilute the entire reaction system to 10 mL with distilled water; return to room temperature after 70 °C water bath (10 min), and measure the absorbance at 765 nm with a microplate reader. Using gallic acid as the equivalent to make a standard curve, the total phenolic content of the sample is expressed as: mg gallic acid / g dry weight of the extract, and the results are shown in Table 1;
[0018] Content of total flavonoids in the samples of each component of P. japonica: dissolve the freeze-dried extract with 70% ethanol, take 1 mL of the sample solution in a centrifuge tube, add 2.5 mL of 70% ethanol, ...
Embodiment 3
[0023] UHPLC-ESI-MS was used to analyze the composition of the compounds in each component of the green thorn fruit, and the specific operation was as follows: the chromatographic conditions of the liquid phase were Reprospher 100 C18 analytical column (100 × 2 mm, 1.8 μm), and the mobile phase was 2.0% formic acid Water (A) and mass spectrometry grade acetonitrile (B), a suitable mobile phase gradient is: 0.01–5 min, 10% B; 5–8 min, 10%–15% B, 8–15 min, 15%–25 % B, 15–20 min, 25%–10% B, 20–23 min, 10% B. The flow rate was 0.2 mL / min, the injection volume was 2.0 µL, and the column temperature was set at 35 °C. The mass spectrometry conditions were: full scan (150-1000 m / z); capillary temperature 320 °C; ionization voltage 3.3 kV; auxiliary gas flow rate 8 L / min; sheath gas flow rate 32 L / min; 350°C. Total phenols and flavonoids were detected in negative ion mode, anthocyanins were detected in positive ion mode, and the detection results of main phenolic substances are shown...
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