Double-label joint design method and preparation method thereof

Abstract

The invention discloses a double-label joint design method and a preparation method thereof. The invention provides a kit for constructing a DNA molecular sequencing library to be detected. The kit comprises a double-sample label joint, wherein the double-sample label joint is formed by annealing a joint sequence L and a joint sequence S; wherein one end of the double-sample label joint is used for connecting a DNA molecule to be detected; compared with the prior art, the method has the following advantages: (1) new sample tags are introduced to two ends adjacent to inserted DNA fragments, sothat the adding frequency of sequencing primers is reduced, and the sequencing cost is reduced; 2) under the condition that the sequencing cost is not increased, a single-ended sequencing project canrealize double sample labels, so that the false positive problem caused by sample label crosstalk is avoided, and the joint design scheme can realize double sample labels by single-ended sequencing and can realize filtering of wrong sequencing data generated by sample label crosstalk.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a design method and a preparation method of a double-label joint. Background technique [0002] At present, high-throughput sequencing technology has become an important genetic detection technology, which is widely used in scientific research, medical detection, agricultural breeding and judicial identification and other fields. At present, mainstream high-throughput sequencing technology providers include Illumina, Thermo Fisher, Pacbio in the United States, nanopore in the United Kingdom, and BGI in China. In order to reduce the average sequencing cost of samples, in most cases, a strategy of mixing and sequencing multiple sample libraries will be adopted. During the library construction process, a sample index (index) is added to each sample, and the sequencing data can be split into each sample according to the sample index, finally achieving high-throughput and low...

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Problems solved by technology

[0006] The existing two-sample label design scheme has the following disadvantages: 1) In order to realize the data acquisition of the double-sample label, it is necessary to add index sequencing primers twice, which increases the sequencing cost; 2) because the sequencing templates of the two sample labels are There are two strands of the DNA library, so the synthesis of the template strand is required before the second sample index sequencing, resulting in increased sequencing time; 3) The current double-sample index design scheme is not compatible with single-end sequencing

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