Application of hickory endocarp extract in preparation of anti-ovarian cancer products

A technology of hickory nut and endocarp, applied in the field of application of hickory nut endocarp extract in the preparation of anti-ovarian cancer products, can solve the problem of anti-cancer and ovarian cancer of hickory nut endocarp that has not been seen, and achieve good anti-cancer activity , the effect of high selective toxicity

Active Publication Date: 2020-10-13
ZHEJIANG FORESTRY UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, there has been no report on the use of pecan endocarp to fight cancer, especially ovarian cancer

Method used

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  • Application of hickory endocarp extract in preparation of anti-ovarian cancer products
  • Application of hickory endocarp extract in preparation of anti-ovarian cancer products
  • Application of hickory endocarp extract in preparation of anti-ovarian cancer products

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Embodiment 1: the preparation of pecan endocarp extract

[0042] (1) Selection and processing of raw materials

[0043] Select Linan local hickory nuts that are fully mature, smooth, crack-free, and odor-free. Soak the hickory nuts in warm water at about 40°C for 30 minutes, so that the seeds and seed coats can be peeled off more conveniently. Remove the shell to separate the pulp from the shell (exocarp and mesocarp). After obtaining the hickory nut kernel, the seeds and the seed coat are manually peeled off. Mesh sieve to obtain hickory nut endocarp powder, seal it with a sealed bag, and store it at -80°C for later use.

[0044] (2) Preparation of Hickory nut endocarp extract

[0045] The hickory nut endocarp powder was dissolved with 80v / v% methanol aqueous solution according to the ratio of material to liquid 1g:10ml, and extracted with the aid of ultrasonic waves, the extraction power was 240W, the extraction temperature was 25°C, and the extraction time was 2h. ...

Embodiment 2

[0046] Example 2: Inhibitory Effect of Hickory Nut Endocarp Extract on Ovarian Cancer Cell Proliferation

[0047] Take 100uL of human ovarian cancer cell OVCAR-3 and normal ovarian cell IOSE 364, inoculate 10,000 cells / well in a 96-well plate (medium is RPMI-1640+10v / v% FBS), and incubate at 37°C, 5%CO 2Incubate for 16 h to attach the cells, remove the medium, and wash once with PBS buffer (0.01M, pH 7.4). The culture medium (the culture medium is RPMI-1640+10v / v% FBS) containing different concentrations of pecan endocarp extract was treated for 24h. Then remove the medium, wash twice with PBS buffer (0.01M, pH7.4), add 100 μL of freshly prepared AQueous One detection solution, incubate at 37°C for 1 h, and use a microplate reader to perform cell viability analysis at 490 nm.

[0048] The experiment used the AQueous One method to determine the inhibitory effect of hickory nut endocarp extract on human ovarian cancer cell OVCAR-3 and ovarian normal cell IOSE 364. Such as fi...

Embodiment 3

[0049] Example 3: Pecan endocarp extract induces apoptosis of ovarian cancer cells through p53-dependent endogenous pathway

[0050] Alexa Flour 488 Annexin (AnnexinV) Apoptosis Kit (Invitrogen) was used to detect the effect of hickory nut endocarp extract on apoptosis of ovarian cancer cells. OVCAR-3 and IOSE 364 cells were treated with 10 6 The concentration per dish was inoculated in a 60mm culture dish (the medium was RPMI-1640+10v / v% FBS), at 37°C, 5% CO 2 Incubate for 16 h, remove the medium. Then add the culture medium (the culture medium is RPMI-1640+10v / v%FBS) containing different concentrations of hickory nut endocarp extract at 37 ℃, 5% CO 2 After cultured for 24 hours, the cells were collected, stained by Annexin V / PI double staining method, and analyzed by flow cytometry after staining. Such as Figure 2-Figure 4 As shown, the increase in apoptosis of OVCAR-3 cells after hickory endocarp extract treatment was dose-dependent. When treated with 20μg / mL pecan en...

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Abstract

The invention discloses application of a hickory endocarp extract in preparation of anti-ovarian cancer products. The hickory endocarp extract is prepared by the following steps: (1) selecting fully mature hickory with smooth and crack-free surface and without peculiar smell, shelling the hickory, and peeling off the hickory endocarp; (2) crushing the hickory endocarp to obtain hickory endocarp powder; (3) extracting the hickory endocarp powder with a methanol aqueous solution to obtain a methanol extract; and (4) filtering and drying the methanol extract to obtain the hickory endocarp extractwith anti-ovarian cancer activity. The extract disclosed by the invention shows higher selective toxicity to human ovarian cancer cells: OVCAR-3, and has good anti-cancer activity. The extract can beexpected to be developed into anti-ovarian cancer products, endows new development and utilization values to hickory, and plays a positive role in promoting the development of hickory industry and pharmaceutical industry.

Description

technical field [0001] The invention relates to the technical fields of extraction of plant active ingredients and biomedicine, in particular to an application of hickory nut endocarp extract in preparation of ovarian cancer-resistant products. Background technique [0002] Ovarian cancer is the second most common female reproductive malignancy, and its mortality rate ranks first among gynecological tumors. Globally, about 230,000 people are diagnosed with ovarian cancer each year, causing 150,000 deaths each year (S. Lheureux, M. Braunstein, A. M. Oza Epithelial ovarian cancer: evolution of management in the era of precision medicine, CA Cancer J. Clin., 69(2019), pp.280-304.). At present, the treatment of ovarian cancer mainly adopts surgery and chemotherapy, the effect is limited, and the 5-year and 10-year survival rates of patients are only 32% and 15% (KimJ, Park EY, Kim O, Schilder JM, Coffey DM, Cho CH, et al. Cell origins of high-gradeserous ovarian cancer. Cancer...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/52A61P35/00A23L33/105
CPCA23V2002/00A61K36/52A61K2236/15A61K2236/333A61K2236/39A61K2236/51A23L33/105A61P35/00A23V2200/308
Inventor 何志平吴峰华叶林林刘兴泉刘畅吴绍珍王乾依林菊
Owner ZHEJIANG FORESTRY UNIVERSITY
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